Anti-Tn Monoclonal Antibody Attenuates Hyperoxia-Induced Lung Injury by Inhibiting Oxidative Stress and Inflammation in Neonatal Mice

Abstract

Maternal immunization with Tn vaccine increases serum anti-Tn antibody titers and attenuates hyperoxia-induced lung injury in neonatal rats. This study determined whether anti-Tn monoclonal antibody can protect against hyperoxia-induced lung injury in neonatal mice. Newborn BALB/c mice were exposed to room air (RA) or normobaric hyperoxia (85% O 2) for 1 week, creating four study groups as follows: RA + phosphatebuffered saline (PBS), RA + anti-Tn monoclonal antibody, O 2 + PBS, and O 2 + anti-Tn monoclonal antibody. The anti-Tn monoclonal antibody at 25 mg/g body weight in 50 ml PBS was intraperitoneally injected on postnatal days 2, 4, and 6. Hyperoxia reduced body weight and survival rate, increased mean linear intercept (MLI) and lung tumor necrosis factor-a, and decreased vascular endothelial growth factor (VEGF) expression and vascular density on postnatal day 7. Anti-Tn monoclonal antibody increased neonatal serum anti-Tn antibody titers, reduced MLI and cytokine, and increased VEGF expression and vascular density to normoxic levels. The attenuation of lung injury was accompanied by a reduction in lung oxidative stress and nuclear factor-kB activity. Anti-Tn monoclonal antibody improves alveolarization and angiogenesis in hyperoxia-injured newborn mice lungs through the suppression of oxidative stress and inflammation.

Anti-Tn Monoclonal Antibody Ameliorates Hyperoxia-Induced Kidney Injury by Suppressing Oxidative Stress and Inflammation in Neonatal Mice

“…The hyperoxia groups were placed in an environment with 85% O 2 for 1 week, and the RA groups were kept in a normoxic environment for 1 week. Anti-Tn monoclonal antibodies were generated and administered as described previously [ 13 ]. The anti-Tn monoclonal antibody-treated groups were intraperitoneally injected with anti-Tn monoclonal antibodies at 25 μ g/g body weight in 50 μ L phosphate-buffered saline (PBS) on postnatal days 2, 4, and 6.…”

“…The dosage was not evaluated for kidney injury in the murine pups exposed to postnatal hyperoxia. We chose this dosage because it increased anti-Tn antibody serum levels 8~10 folds higher in treated group over PBS group on postnatal day 7 [ 13 ]. The PBS-treated groups were injected with equivalent amounts of PBS on the same days.…”

“…The kidneys were excised for histological, Western blot, and cytokine analyses. The kidneys used for these experiments were obtained from a previous study designed to assess lung injury [ 13 ].…”

Bone Marrow Mesenchymal Stem Cells-derived Exosomes Promote Survival of Random Flaps in Rats through Nrf2-mediated Antioxidative Stress

“…which promotes inflammation and vascular endothelial cell dysfunction, aggravating pathological processes and forming a vicious cycle 3 . Supplementation with exogenous antioxidants has a poor effect on OS 4 .…”

Procyanidin B2 regulates the Sirt1/Nrf2 signaling pathway to improve random‐pattern skin flap survival

“…If a random-pattern skin flap is used during surgery, the number of axial vessels will determine whether the flap's metabolic needs can be met; insufficient axial arteries frequently result in ischemia and hypoxic injuries (Myers & Cherry, 1968). In addition, hyperoxia may trigger an inflammatory response, which exacerbates oxidative damage (Chen et al, 2020). Angiogenesis facilitates the restoration of blood supply and provides nutritional support (Qing et al, 2019).…”