Anti-Trypanosoma, anti-Leishmania and cytotoxic activities of natural products from Psidium brownianum Mart. ex DC. and Psidium guajava var. Pomifera analysed by LC–MS

Souza, Celestina Elba Sobral de; Silva, Ana Raquel Pereira da; Gómez, María Celeste Vega; Rolóm, Míriam; Coronel, Cathia; Costa, José Galberto Martins da; Sousa, Amanda Karine de; Rolim, Larissa Araújo; Souza, Francisco Hugo Sobral de; Coutinho, Henrique Douglas Melo

doi:10.1016/j.actatropica.2017.09.009

Cited by 22 publications

(10 citation statements)

References 40 publications

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“…In this study, selenium niosome, selenium plus glucantime niosome or glucantime plus selenium showed lower cytotoxicity as compared with selenium alone. This result is in agreement with other previous studies which reported that Se NPs demonstrated low toxicity [28,29]. This result also showed that niosomal formulation had the least cytotoxicity because of its targeting delivery of drugs.…”

Section: Discussionsupporting

confidence: 93%

A Novel Niosomal Combination of Selenium Coupled with Glucantime against Leishmania tropica

et al. 2019

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There is no effective treatment modality available against different forms of leishmaniasis. Therefore, the aim of this study was to improve the penetration and efficacy of selenium and glucantime coupled with niosomes and compared them with their simple forms alone on in vitro susceptibility assays. In this study, the niosomal formulations of selenium and in combination with glucantime were prepared. The size and morphology of the niosomal formulations were characterized and the effectivity of the new formulation was also evaluated using in vitro MTT assay, intra-macrophage model, and gene expression profile. From the results obtained, no cytotoxicity effect was observed for niosomal and simple forms of drugs, as alone or in combination. Niosomal formulations of the drugs significantly showed more inhibitory effects ( P ≤0.001) than the simple drugs when the selectivity index was considered. The gene expression levels of Interleukin (IL-10) significantly decreased, while the level of IL-12 and metacaspase significantly increased ( P ≤0.001). The results of the present study showed that selenium plus glucantime niosome possess a potent anti-leishmanial effect and enhanced their lethal activity as evidenced by the in vitro experiments.

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Section: Discussionsupporting

confidence: 93%

A Novel Niosomal Combination of Selenium Coupled with Glucantime against Leishmania tropica

et al. 2019

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“…Biological assays revealed that leaf extracts exhibit antibacterial and antifungal activity against Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans [12]. Previous studies have described its antileishmanial potential, showing a 90% and 75% inhibition of L. donovani and L. infantum promastigotes, respectively [13,14]. However, to date, antileishmanial activity of purified active compounds from guava has not been reported.The purpose of this study was to find new antileishmanial compounds from leaf extracts of P. guajava L. using a metabolomic approach and correlation analysis.…”

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confidence: 99%

Antileishmanial Compounds Isolated from Psidium Guajava L. Using a Metabolomic Approach

et al. 2019

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With an estimated annual incidence of one million cases, leishmaniasis is one of the top five vector-borne diseases. Currently available medical treatments involve side effects, including toxicity, non-specific targeting, and resistance development. Thus, new antileishmanial chemical entities are of the utmost interest to fight against this disease. The aim of this study was to obtain potential antileishmanial natural products from Psidium guajava leaves using a metabolomic workflow. Several crude extracts from P. guajava leaves harvested from different locations in the Lao People's Democratic Republic (Lao PDR) were profiled by liquid chromatography coupled to high-resolution mass spectrometry, and subsequently evaluated for their antileishmanial activities. The putative active compounds were highlighted by multivariate correlation analysis between the antileishmanial response and chromatographic profiles of P. guajava mixtures. The results showed that the pooled apolar fractions from P. guajava were the most active (IC 50 = 1.96 ± 0.47 µg/mL). Multivariate data analysis of the apolar fractions highlighted a family of triterpenoid compounds, including jacoumaric acid (IC 50 = 1.318 ± 0.59 µg/mL) and corosolic acid (IC 50 = 1.01 ± 0.06 µg/mL). Our approach allowed the identification of antileishmanial compounds from the crude extracts in only a small number of steps and can be easily adapted for use in the discovery workflows of several other natural products.Molecules 2019, 24, 4536 2 of 13 of the human population to the parasite, and human behavior [3]. The disease is characterized by four forms; visceral leishmaniasis, post-kala-azar dermal leishmaniasis, cutaneous leishmaniasis, and mucocutaneous leishmaniasis [4]. Available medical treatments are not sufficient because of their extensive toxicity (e.g., the nephrotoxicity of amphotericin B, the teratogenicity of miltefosine), their lack of efficacy, their expensive cost (amphotericin B), the development of drug resistance (sodium stibogluconate and meglumine antimoniate), and non-specific targeting [4,5]. Fexinidazole, a 5-nitroimidazole, was recently in phase II of clinical trials on visceral leishmaniasis but exhibited a lack of efficacy, and in 2018 no new lead molecules were included in clinical trials [4].Control of leishmaniasis by traditional approaches (vector control and treatment of patients) is facing the difficulties of identifying new cost-effectively produced bioactive molecules able to be produced on an industrial scale, rapid development of drug resistance, and the lack of access to drugs by the threatened populations. These issues are at the heart of concerns of the WHO regarding the control of tropical diseases [6]. Therefore, combination therapies are used to improve the efficacy of antileishmanial therapies and can shorten treatment duration [7]. Furthering our fundamental knowledge through original approaches, herein concerning the activity of natural compounds from a promising plant species active against Leishmania spp., is vital to ov...

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“…Therefore, this study was conducted to test doses of 100 mg/mL to evaluate the antiparasitic activity. Additionally, previous studies have shown parasitic inhibition of 75% and 90% for L. infantum and L. donovani promastigotes, respectively [29,30], although it is known that the promastigote model is not suitable for testing antiparasitic drugs because it is a model that is not found in mammalian infection. Other species of the genus Psidium such as the ethanolic extract of P. brownianum leaves and the essential oils of P. myrsinites leaves have been tested against L. braziliensis and L. infantum promastigotes with moderate antiparasitic effects, reaching up to 81.34% antiparasitic activity [29,31].…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, previous studies have shown parasitic inhibition of 75% and 90% for L. infantum and L. donovani promastigotes, respectively [29,30], although it is known that the promastigote model is not suitable for testing antiparasitic drugs because it is a model that is not found in mammalian infection. Other species of the genus Psidium such as the ethanolic extract of P. brownianum leaves and the essential oils of P. myrsinites leaves have been tested against L. braziliensis and L. infantum promastigotes with moderate antiparasitic effects, reaching up to 81.34% antiparasitic activity [29,31]. In contrast, in this study, the ethanolic extract (100mg/mL) of P. guajava leaves reached an antiparasitic effect of 22.58% in promastigotes, although in axenic amastigotes it reached 91.67% of parasitic death.…”

Section: Resultsmentioning

confidence: 99%

Antiparasitic effect of Psidium guajava on promastigotes and axenic amastigotes of Leishmania

Rojas‐Jaimes

Mesía-Guevara

Rojas-Puell

et al. 2022

Preprint

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Background: Leishmaniasis is a stigmatic and mutilating disease due to pathogenic species of the genus Leishmania which, depending on the species and the individual's immune status, may vary clinically from a cutaneous, mucosal, and visceral form, and for which there is no suitable treatment without significant side effects. Methods: The method of [3-(3,4 -dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] was used to study the antiparasitic effects of ethanolic (100mg/mL) and aqueous (100mg/mL) extracts of Psidium guajava on axenic amastigotes cultures (8.1 x103 parasite/mL) and promastigotes (12 x 104 parasite/mL) obtained from a patient with cutaneous leishmaniasis, and the percentage of parasite death was evaluated in comparison with Glucantime (300mg/mL) and untreated parasite cultures. Results: Regarding parasite death in promastigotes, the ethanolic and aqueous extracts had a percentage of 22.58% and -45.16%, respectively, with no significant difference between treatments (N=3) (p= 0.058). In contrast, the ethanolic and aqueous extracts had an antiparasitic percentage of 91.67% and -70.83%, respectively, with a significant difference between treatments (N=3) (p<0.05). Conclusions:Our study showed high and significant effectiveness in parasite death (91.67%) of Leishmania axenic amastigotes of the ethanolic extract (100mg/mL) of Psidium guajava, being this result promising and the basis for in vivo studies, using the ethanolic extraction of P. guajava.

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Anti-Trypanosoma, anti-Leishmania and cytotoxic activities of natural products from Psidium brownianum Mart. ex DC. and Psidium guajava var. Pomifera analysed by LC–MS

Cited by 22 publications

References 40 publications

A Novel Niosomal Combination of Selenium Coupled with Glucantime against Leishmania tropica

A Novel Niosomal Combination of Selenium Coupled with Glucantime against Leishmania tropica

Antileishmanial Compounds Isolated from Psidium Guajava L. Using a Metabolomic Approach

Antiparasitic effect of Psidium guajava on promastigotes and axenic amastigotes of Leishmania

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