Anti-Tumor and Anti-Inflammatory Activity In Vivo of Apodanthera congestiflora Cogn. (Cucurbitaceae)

Abstract

This work aimed to carry out a study of Apodanthera congestiflora by investigating its chemical composition and pharmacological potential. From the dichloromethane phase (Dic-Ac) of the A. congestiflora stems, three compounds were identified: cayaponoside C5b (Ac-1), cabenoside C (Ac-2) and fevicordin C2 glucoside (Ac-3), being last identified for the first time as a natural product. These compounds were obtained by chromatographic methods and their structures were elucidated by means of spectroscopic analysis of IR, MS and NMR. In the quantification of Dic-Ac, it was possible to observe the presence of 7% of cayaponoside C5b. Dic-Ac showed significant toxicity for in vivo tests, with macroscopic and biochemical changes. The anti-inflammatory activity of Dic-Ac was investigated using the paw edema model. A decrease in inflammatory signs was observed in the first 5 h and the most effective dose in reducing edema with was 7.5 mg kg−1 (66.6%). Anti-tumor activity of Dic-Ac was evaluated by Ehrlich’s carcinoma model, which showed inhibition rate of 78.46% at 15 mg kg−1 dosage. The phytochemical investigation, together with the biological tests carried out in this study, demonstrated that A. congestiflora is a promising species in the search for therapeutics, since it contains substances with high pharmacological potential in its composition.

Antifungal Sesquiterpenoids from Michelia formosana Leaf Essential Oil against Wood-Rotting Fungi

“…The structural determination of isolated compounds was performed by spectral analyses, including 1D NMR (Nuclear magnetic resonance spectroscopy) ( 1 H-NMR, 500 MHz; 13 C-NMR, 125 MHz) and 2D NMR (HSQC, HMBC, COSY, and NOESY) measured on a Bruker AVIII NMR spectrometer (Bruker Avance, Rheinstetten, Germany), FTIR (Fourier transform infrared spectroscopy, FTS-40, Bio-rad, Hercules, CA, USA), and MS (mass spectroscopy, MAT-958, Finnigan, MA, USA) [ 37 , 38 , 39 , 40 ].…”

Xanthine Oxidase Inhibitory Activity and Chemical Composition of Pistacia chinensis Leaf Essential Oil

“…P. chinensis leaf essential oil (50 g) was further subjected to classical preparative silica gel column chromatography (CC) with a gradient elution of n -hexane and ethyl acetate of increasing polarity, and then separated into six fractions (E1–E6) using thin-layer chromatography (TLC) to analyze the elution profiles at both 254 nm and 365 nm UV light [ 41 , 42 , 43 ].…”

Antimelanogenesis Effects of Leaf Extract and Phytochemicals from Ceylon Olive (Elaeocarpus serratus) in Zebrafish Model

“…The structures of isolated compounds were determined and characterized by means of spectral analyses, including ultraviolet-visible spectroscopy (UV/VIS, V-550, Jasco, Tokyo, Japan), Fourier transform infrared spectroscopy (FTIR, FTS-40, Bio-rad, Hercules, CA, USA), and mass spectroscopy (MS, MAT-958, Finnigan, MA, USA). Nuclear magnetic resonance spectroscopy (NMR), including 1D ( 1 H-NMR, 500 MHz; 13 C-NMR, 125 MHz) and 2D NMR (HSQC and HMBC), were recorded with a Bruker AVIII NMR spectrometer (Bruker Avance, Rheinstetten, Germany) [ 29 , 30 , 31 , 32 ].…”