Anti‐Tumor Effect of Copper Sulfide Nanoparticles Carrying siRNA and Adriamycin

Abstract

A combination of treatment strategies with two or more different mechanisms can together prevent cancer progression. In this paper, we present a combination of strategies with siRNA interference-induced gene silencing, photothermal therapy and mesoporous drug delivery methods to cure cancer. First we synthesize oil-soluble copper sulfide nanoparticles, which are modified with mesoporous silica and (3-Amino-propyl)triethoxysilane (APTES), then use it as siRNA carrier to achieve siRNA transmission. At the same time, mesopores can also be used for drug delivery. Our results suggest that the copper sulfide nanomaterial loaded with siRNA and Adriamycin entered the cell successfully, and the composite can kill cancer cells effectively.[a] W.

Redox-responsive mesoporous silica nanoparticles for chemo- photodynamic combination cancer therapy

“…Photodynamic therapy is the basic principle of photodynamic therapy, especially singlet oxygen ( 1 O 2 ), activating photosensitizers under laser conditions to produce highly cytotoxic ROS [6]. As a detection agent, the fluorescent probe DCFH-DA was used to detect the intracellular ROS levels of MCF-7 cells by fluorescence microscopy and flow cytometry.…”

“…The PDT therapeutic effect in cells was investigated with a DCFH-DA probe. However, the probe itself is non-fluorescent, and it can be converted to high fluorescence intensity 2′,7′-Dichlorofluorescein (DCF) after reacting with ROS [6]. The probe solution was diluted at a 1:1000 ratio, and the prepared probe solution was kept away from light and placed at 4 °C for later use.…”

“…Doxorubicin (DOX) is a broadspectrum anticancer drug that treats malignant tumors by provoking long-term disruption of DNA replication [3]. Like many chemotherapeutics, DOX also has shortcomings, such as non-targeting and poor water solubility [6][7][8], leading to only a relatively small fraction of drugs delivered and accumulated in tumor tissues through blood circulation [9]. Therefore, traditional chemotherapy shows relatively low targeting effects and high toxicity.…”

“…Single therapy is hard to provide the expected effect. Therefore, combining nanocarrier materials with different treatment methods may improve cancer treatment effects [6]. Photodynamic therapy (PDT) is a widely studied exogenous stimulation therapy that requires light, photosensitizers, and molecular oxygen [25][26][27][28].…”

Engineering of bioactive metal sulfide nanomaterials for cancer therapy

“…Efficient drug delivery capabilities make MeSNs more attractive drug carriers in the field of tumor treatment. Although most of MeSNs are solid without internal spaces for loading drugs, these nanomaterials can still achieve efficient delivery and control the release of small molecules (such as DOX or siRNA) after coating with mesoporous silica or organic polymers shell [ 89 , 90 ].…”