Anti-ulcerogenic potential of anthocyanin-loaded chitosan nanoparticles against alcohol-HCl induced gastric ulcer in rats

Raman, Sreerekha Perumcherry; Dara, Pavan Kumar; Vijayan, Divya K; Chatterjee, Niladri Sekhar; Raghavankutty, Mahadevan; Mathew, Suseela; Ravishankar, C.N.; Anandan, R.

doi:10.1080/14786419.2020.1860041

Cited by 15 publications

(7 citation statements)

References 12 publications

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“…Chitosan was used as a reducing and stabilizing agent, whereas TPP was used as a cross‐linking agent. The chitosan‐to‐TPP ratio (w/w 1:0.5) for ionic gelation was similar to earlier reports 14 . Briefly, anthocyanin was dissolved in 1% (w/v) of low‐molecular‐weight chitosan solution at a concentration of 1 mg mL −1 .…”

Section: Methodssupporting

confidence: 56%

Nanoencapsulation in low‐molecular‐weight chitosan improves in vivo antioxidant potential of black carrot anthocyanin

et al. 2021

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BACKGROUND Anthocyanins are flavonoids that are potential antioxidant, anti‐inflammatory, anti‐obesity, and anti‐carcinogenic nutraceutical ingredients. However, low chemical stability and low bioavailability limit the use of anthocyanins in food. Nanoencapsulation using biopolymers is a recent successful strategy for stabilization of anthocyanins. This study reports the development, characterization, and antioxidant activity of black carrot anthocyanin‐loaded chitosan nanoparticles (ACNPs). RESULTS The ionic gelation technique yielded the ACNPs. The mean hydrodynamic diameter d and polydispersity index PDI of chitosan nanoparticles and ACNPs were found to be d = 455 nm and PDI = 0.542 respectively for chitosan nanoparticles and d = 274 nm and PDI = 0.376 respectively for ACNPs. The size distribution was bimodal. The surface topography revealed that the ACNPs are spherical and display a coacervate structure. Fourier transform infrared analysis revealed physicochemical interactions of anthocyanins with chitosan. The loading process could achieve an encapsulation efficiency of 70%. The flow behavior index η of encapsulated ACNPs samples revealed Newtonian and shear thickening characteristics. There was a marginal reduction in the in vitro antioxidant potential of anthocyanins after nanoencapsulation, as evidenced from 2,2‐diphenyl‐1‐picrylhydrazyl, ferric reducing antioxidant power, and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) assays. Interestingly, the in vivo antioxidant potential of anthocyanins improved following nanoencapsulation, as observed in the serum antioxidant assays. CONCLUSION The optimized nanoencapsulation process resulted in spherical nanoparticles with appreciable encapsulation efficiency. The nanoencapsulation process improved the in vivo antioxidant activity of anthocyanins, indicating enhanced stability and bioavailability. The promising antioxidant activity of the ACNPs suggests a potential for utilization as a nutraceutical supplement. © 2021 Society of Chemical Industry.

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Section: Methodssupporting

confidence: 56%

Nanoencapsulation in low‐molecular‐weight chitosan improves in vivo antioxidant potential of black carrot anthocyanin

et al. 2021

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“…Moreover, encapsulation allows anthocyanins to perform various functions to a wider extent. For example, anthocyanin-loaded chitosan nanoparticles can alleviate inflammation of gastric ulcers in rats caused by alcohol . All anthocyanins were detected at relatively high concentrations in stomach tissue after 0.25 h, reaching a maximum at 1 h after treatment, with C3GA being present in the highest concentration in the CG (451.22 ± 92.78 ng/g, Figure a).…”

Section: Resultsmentioning

confidence: 97%

“…For example, anthocyanin-loaded chitosan nanoparticles can alleviate inflammation of gastric ulcers in rats caused by alcohol. 33 All anthocyanins were detected at relatively high concentrations in stomach tissue after 0.25 h, reaching a maximum at 1 h after treatment, with C3GA being present in the highest concentration in the CG (451.22 ± 92.78 ng/g, Figure 5a). In the case of the EG, APNPs provided the best protection throughout the process in all tissues, particularly for C3A at 1.5 h in the stomach; the difference between the concentrations of C3A in the EG and CG was 130.95 ng/g, with the concentration of C3A in the EG being more than double that in the CG.…”

Section: Digestive Tissues (Stomach and Intestine)mentioning

confidence: 96%

Anthocyanins from Aronia melanocarpa Bound to Amylopectin Nanoparticles: Tissue Distribution and In Vivo Oxidative Damage Protection

Tong

Meng

2022

J. Agric. Food Chem.

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The in vivo applications of anthocyanins are limited by their instability. Nano-encapsulation using amylopectin nanoparticles (APNPs) stabilizes anthocyanins to deliver them to tissues to ameliorate their physiological functions. Herein, rats are fed four Aronia melanocarpa anthocyanins encapsulated with APNPs, and their subsequent distributions and bioactivity in nine tissues are revealed using UHPLC−MS. Among digestive tissues, the concentration of the APNP-protected cyanidin 3-O-arabinoside in the stomach is 134.54% of that of the free anthocyanin, while among non-digestive tissues, the APNP-protected cyanidin 3-Oglucoside concentration in the lungs improved by 125.49%. Concentration maxima "double peaks" in the liver and kidney arise from different modes of transport. Sustained release of anthocyanins from anthocyanin-APNPs and stable concentration curves suggest controlled delivery, with most APNPs consumed in the digestive system. APNPs did not affect the overall anthocyanin absorption time or tissues. The superoxide dismutase and malondialdehyde concentrations indicate that APNPs enhance the oxidative damage protection in vivo.

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“…Eye health, cardiovascular diseases, , antiobesity, , antidiabetic, antibacterial, anticancer, or neuroprotective effects attributable to dietary intake of anthocyanin-rich foods have been well shown in research using experimental models. Moreover, anthocyanins isolated from the different plant sources have been reported for their antiulcer and gastroprotective activities in different animal models. − …”

Section: Introductionmentioning

confidence: 99%

Effects of the Anthocyanin Hirsutidin on Gastric Ulcers: Improved Healing through Antioxidant Mechanisms

et al. 2022

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The goal of this study was to determine the effect of hirsutidin on ethanol-induced stomach ulcers in rats. Rats (n = 24 rats/group) were separated at random into the following groups: normal saline-treated (normal control), ethanol-treated (ethanol control), 10 mg/kg hirsutidin + ethanol-treated (hirsutidin 10), and 20 mg/kg hirsutidin + ethanol-treated (hirsutidin 20). All the groups received the respective treatment orally for 7 days. On day 7, i.e., after 24 h of fasting, except for the normal control group, all the groups orally received 5 mL/kg of ethanol. Four hours later, rats were anaesthetized, serum was isolated from the blood, and biochemical tests were performed. The stomach tissue was utilized for ulcer grading, histology, and biochemical analysis. The rats developed stomach acidity and ulcers after being given ethanol based on increased ulcer score, disturbed cellular architecture, increased oxidative stress, myeloperoxidase and decreased endogenous antioxidants, and nitric oxide and prostaglandin E2 concentration. Ethanol-treated rats also displayed increased tumor necrosis factor-α, aspartate aminotransferase, alanine transaminase, alkaline phosphatase, and inflammatory cytokines. The treatment with hirsutidin protected and significantly restored all serum parameters in ethanol-induced stomach ulcers and may have antiulcer activity.

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Anti-ulcerogenic potential of anthocyanin-loaded chitosan nanoparticles against alcohol-HCl induced gastric ulcer in rats

Cited by 15 publications

References 12 publications

Nanoencapsulation in low‐molecular‐weight chitosan improves in vivo antioxidant potential of black carrot anthocyanin

Nanoencapsulation in low‐molecular‐weight chitosan improves in vivo antioxidant potential of black carrot anthocyanin

Anthocyanins from Aronia melanocarpa Bound to Amylopectin Nanoparticles: Tissue Distribution and In Vivo Oxidative Damage Protection

Effects of the Anthocyanin Hirsutidin on Gastric Ulcers: Improved Healing through Antioxidant Mechanisms

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