Anti-viral RNAi nanoparticles protect shrimp against white spot disease

Ufaz, Shai; Balter, Adi; Tzror, Chen; Einbender, Shai; Koshet, Ori; Shainsky‐Roitman, Janna; Yaari, Zvi; Schroeder, Avi

doi:10.1039/c7me00092h

Cited by 23 publications

(14 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chitosan/dsRNA nanoparticles were first developed to facilitate oral RNAi in Anopheles gambiae larvae 19 . The nanoparticles have been used to facilitate oral RNAi in Aedes aegypti larvae, 32,33 Chilio suppressalis larvae, 30 Chironomus tentans larvae, 34 Penaeus vannamei shrimp, 35 and Caenorhabditis elegans nematodes 36 . In C. suppressalis , chitosan/dsRNA nanoparticles enhanced dsRNA stability and uptake, resulting in enhanced RNAi efficiency 30 .…”

Section: Discussionmentioning

confidence: 99%

Comparison of strategies for enhancing RNA interference efficiency in Ostrinia nubilalis

Cooper

Song

et al. 2020

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND Targeting insect‐specific genes through post‐transcriptional gene silencing with RNA interference (RNAi) is a new strategy for insect pest management. However, lepidopterans are recalcitrant to RNAi, which prevents application of novel RNAi technology to many notorious pests, including Ostrinia nubilalis (ECB). Strategies for enhancing RNAi efficiency, including large doses of double‐stranded RNA (dsRNA), nuclease inhibitors, transfection reagents, and nanoparticles, have proved useful in other insects exhibiting substantial dsRNA degradation, a major mechanism limiting RNAi efficacy. To determine if similar strategies can enhance RNAi efficiency in ECB, various reagents were tested for their ability to enhance dsRNA stability in ECB tissues, then compared for their effectiveness in whole ECB. RESULTS Ex vivo incubation experiments revealed that Meta dsRNA lipoplexes, EDTA, chitosan‐based dsRNA nanoparticles, and Zn2+ enhanced dsRNA stability in ECB hemolymph and gut content extracts, compared with uncoated dsRNA. Despite these positive results, the reagents used in this study were ineffective at enhancing RNAi efficiency in ECB in vivo. To reduce assay time and required dsRNA, midguts were dissected and incubated in tissue culture medium containing dsRNA with and without reagents. These experiments showed that RNAi efficiency varied between target genes, and nuclease inhibitors improved RNAi efficiency for only a portion of the refractory target genes investigated ex vivo. CONCLUSION These results indicate that enhancing dsRNA stability is insufficient to improve RNAi efficiency in ECB and suggests the existence of additional, complex mechanisms contributing to low RNAi efficiency in ECB.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of strategies for enhancing RNA interference efficiency in Ostrinia nubilalis

Cooper

Song

et al. 2020

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…Other studies by IM injection of chemically modified chitosan nanoparticles loaded with anti-VP28 RNA [20] and antisense plasmid constructs for VP28 [24] have shown protection of 95% and 90%, respectively. However, in all these treatments the shrimp exposed to WSSV finally died at 14 dpi.…”

Section: Discussionmentioning

confidence: 95%

“…The antiviral response of RNAi is triggered by double-stranded RNA (dsRNA) to block the synthesis of a specific viral protein, in the case of WSSV the targets being the structural proteins VP19, VP24, VP26, and VP28, as they are involved in cell recognition, virus entry, binding and assembly of the virion. Previous studies have shown that silencing these structural proteins in WSSV challenge assays, increases shrimp survival [10,11,[17][18][19][20][21]. The VP28 glycoprotein plays an important role in systemic infection by interacting with cell membrane proteins, and it is one of the most abundant proteins along with VP26 (≈60%) in the external WSSV surface [21,22].…”

Section: Introductionmentioning

confidence: 99%

“…However, RNAi intramuscular (IM) administration is limited to lab-scale experiments since its use is not yet viable for applications on a large scale, as found in salmon farms [25]. The naked RNA degrades quickly when supplied in feed [26,27], either due to feed processing or the digestion process [20]. The challenge is to develop a treatment through the oral route [11,28] instead of IM, yet one in which the RNA is nonetheless is protected.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antiviral therapy in shrimp through plant virus VLP containing VP28 dsRNA against WSSV

Ramos-Carreño¹,

Giffard‐Mena²,

Zamudio-Ocadiz³

et al. 2021

Beilstein J. Org. Chem.

View full text Add to dashboard Cite

The white spot syndrome virus (WSSV), currently affecting cultured shrimp, causes substantial economic losses to the worldwide shrimp industry. An antiviral therapy using double-stranded RNA interference (dsRNAi) by intramuscular injection (IM) has proven the most effective shrimp protection against WSSV. However, IM treatment is still not viable for shrimp farms. The challenge is to develop an efficient oral delivery system that manages to avoid the degradation of antiviral RNA molecules. The present work demonstrates that VLPs (virus-like particles) allow efficient delivery of dsRNAi as antiviral therapy in shrimp. In particular, VLPs derived from a virus that infects plants, such as cowpea chlorotic mottle virus (CCMV), in which the capsid protein (CP) encapsidates the dsRNA of 563 bp, are shown to silence the WSSV glycoprotein VP28 (dsRNAvp28). In experimental challenges in vivo, the VLPs- dsRNAvp28 protect shrimp against WSSV up to 40% by oral administration and 100% by IM. The novel research demonstrates that plant VLPs, which avoid zoonosis, can be applied to pathogen control in shrimp and also other organisms, widening the application window in nanomedicine.

show abstract

“…7,8 One other proposed approach is to use NANOparticulate RNA interference (RNAi) formulations to downregulate genes that facilitate the viral progression. 9 Although such studies produced promising results in vitro against coronavirus, their efficacy in vivo (as well as in clinic) is yet to be explored. Considering that viruses could be phylogenetically unrelated and structurally different, and given that most vaccines are virus specific, a promising approach would be that of developing broad-spectrum anti-viral NANOparticles to fight COVID-19 and future pandemics.…”

Section: Nano-based Anti-virals As Potential Alternatives?mentioning

confidence: 99%

Why Go NANO on COVID-19 Pandemic?

Talebian

Conde

2020

Matter

View full text Add to dashboard Cite

show abstract

Anti-viral RNAi nanoparticles protect shrimp against white spot disease

Abstract: Nearly 20% of cultured shrimp die every year due to viral diseases. In this study, we evaluated the capacity of nanoparticulate RNA interference (RNAi) to down-regulate genes in Penaeus vannamei shrimp and protect shrimp against white spot syndrome virus (WSSV, i.e. white spot disease).

Cited by 23 publications

References 49 publications

Comparison of strategies for enhancing RNA interference efficiency in Ostrinia nubilalis

Comparison of strategies for enhancing RNA interference efficiency in Ostrinia nubilalis

Antiviral therapy in shrimp through plant virus VLP containing VP28 dsRNA against WSSV

Why Go NANO on COVID-19 Pandemic?

Contact Info

Product

Resources

About