Antiadhesion as a functional concept for prevention of pathogens: N‐Phenylpropenoyl‐L‐amino acid amides as inhibitors of the Helicobacter pylori BabA outer membrane protein

Abstract

The nutritional use of NPA-containing food may justify a positive antiadhesive effect against the recurrence of H. pylori infections.

Fast determination of N-phenylpropenoyl-l-amino acids (NPA) in cocoa samples from different origins by ultra-performance liquid chromatography and capillary electrophoresis

“…Furthermore the aspartic acid amide of caffeic acid (Caff-Asp) showed strong antiadhesive properties against the adhesion of Helicobacter pylori to human stomach tissue (Hensel et al, 2007). It was shown that NPA interact with the H. pylori outer membrane protein BabA (Niehues, Stark, Keller, Hofmann, & Hensel, 2011). Preliminary data on the occurrence and bioactivity in food and medicinal plants (Hensel et al, 2007; and on intestinal absorption and bioavailability in humans (Stark, Lang, Keller, Hensel, & Hofmann, 2008) are available.…”

Effects of polysaccharide isolated from Streptococcus thermophilus CRL1190 on human gastric epithelial cells

“…From these results it is assumed that the polysaccharide may interact with the immunological system. Some pre-and probiotics interact with the bacterial adhesion of pathogens to the eukaryotic binding receptors throughout so called antiadhesive compounds [29][30][31][32][33]. In case of pretreatment of AGS cells with EPS1190 the polysaccharide was shown to inhibit H. pylori adhesion by interaction with the surface of AGS cells (Fig.…”

Polyphenols from Myrothamnus flabellifolia Welw. inhibit in vitro adhesion of Porphyromonas gingivalis and exert anti-inflammatory cytoprotective effects in KB cells

“…It seems interesting that inhibition of the Arg‐gingipain proteases in our in vitro study leads to a feed back mechanism of increased gene expression for rgp A, which indicates that the gingipain activity is controlled by bacterial cellular programmes. Recent studies on correlation of Helicobacter pylori adhesion with the gene expression for the respective OMP adhesins showed that the adhesion process for H. pylori is not related to changes in gene expression of the adhesins and virulence factors (Niehues et al 2010a). In contrast, P. gingvalis seems to have a gene regulation system, which correlates gene expression with gingipain protease activity.…”

“…Owing to the fact that P. gingivalis cell adhesion predominantly relies on the functions of gingipain proteases, the use of anti‐adhesive compounds influencing protein–protein interactions should be favoured. In contrast, carbohydrate–protein interactions, as known for other adhesive gram‐negative bacteria (Niehues et al 2010a), are less important for P. gingivalis binding to eukaryotic cells (M. Niehues, T. Stark, D. Keller, T. Hofmann, A. Hensel unpublished data). Manipulation of the P. gingivalis OMP functions can be achieved either by soluble, exogenous modified substrates of the gingipains, e.g.…”

“…Because of the complex adhesion strategy of P. gingivalis , a multi‐target therapy using complex compound mixtures with affinity against the different bacterial adhesins has to be considered; use of a single compound with inhibitory activity against a single adhesion would probably not be sufficient for an effective inhibition of bacterial attachment to the host cells. During the search for potential anti‐adhesive compounds with affinity to P. gingivalis fimbriae or OMPs, the use of plant‐derived natural products, especially polyphenols and polysaccharides, is a new focus of attention (Niehues et al 2010a). While polymeric carbohydrates interact with bacterial adhesins involved in protein–carbohydrate binding, polyphenols are capable of binding to adhesins, forming protein–protein bounds to the host membrane surface (M. Niehues, T. Stark, D. Keller, T. Hofmann, A. Hensel unpublished data).…”