Antiadhesive Character of Mucin O-glycans at the Apical Surface of Corneal Epithelial Cells

Sumiyoshi, Mika; Ricciuto, Jessica; Tisdale, Ann; Gipson, Ilene K.; Mantelli, Flavio; Argüeso, Pablo

doi:10.1167/iovs.07-1038

Cited by 64 publications

(46 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[23] MUC16 can help in the formation of a disadhesive barrier, [24] and in regulating the mucosal defenses of the epithelial cell layer. [25] CA125 is identified as a 5797 -base pair cDNA isolated from the OVCAR-3 cDNA library.…”

Section: Discussionmentioning

confidence: 99%

CA125 is a potential biomarker to predict surgically incurable gastric and cardia cancer

et al. 2016

View full text Add to dashboard Cite

Preoperative evaluation of the curability of gastric and cardia cancer is important to avoid risks of unnecessary surgery. Our previous study has reported several clinical parameters associated with incurable gastric surgery. In this study, we aimed to evaluate the correlation between CA125 and the curability of gastric and cardia cancer.A total of 297 cases of gastric and cardia cancer were analyzed retrospectively, including 153 cases with radical surgery and 144 with surgery for incurable gastric or cardia cancer. χ2 test was performed to analyze the associations between curability or incurable factors and clinicopathological data, including CA125 value. ROC curves were generated, and cutoff points for curability, T status, N status, peritoneal metastasis, and distant metastasis were found, respectively. Binary logistic regression was performed to verify the associations between dependent variables (curability, T status, N status, peritoneal metastasis, and distant metastasis) and covariates (related clinicopathological data from step 1 and cutoff points from step 2).Esophageal involvement, T grade, and CA125 were risk factors of curability. T grade and Borrmann type were risk factors of T status. T grade and CA125 were risk factors of N status. Age, esophageal involvement, T grade, and CA125 were risk factors of peritoneal metastasis. CA125 was risk factor of distant metastasis.CA125 is a potential biological marker for curability prediction of gastric and cardia cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

CA125 is a potential biomarker to predict surgically incurable gastric and cardia cancer

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The mucins of undifferentiated epithelial cells are under-glycosylated. They lack the carbohydrate mucin antigen H185, which is expressed during differentiation Sumiyoshi et al, 2008).…”

Section: Transcellular Uptake Of Dye and Intercellular Spreadmentioning

confidence: 99%

Clinical staining of the ocular surface: Mechanisms and interpretations

Bron

Argüeso

İrkeç

et al. 2015

Progress in Retinal and Eye Research

140

109

View full text Add to dashboard Cite

“…Using human ocular mucins extracted and purified from human conjunctival tissue using classical mucin purification techniques, Berry et al demonstrated that the number of mucin-mucin interactions is minimal as compared to mucin-mica interactions. More recently, the role of cell surface O-glycans in providing boundary lubrication has been studied using static and dynamic flow adhesion assays in human corneal epithelial cells treated with benzyl-α-GalNAc, a chemical primer commonly used to suppress the elongation of O-glycan chains [21]. These experiments demonstrated that the cell surface character of corneal epithelial cells producing mucin O-glycans is more anti-adhesive than that of cells with altered O-glycans.…”

Section: Roles For Mucin O-glycans In the Glycocalyxmentioning

confidence: 99%

Glycobiology of the ocular surface: mucins and lectins

Argüeso

2013

Jpn J Ophthalmol

View full text Add to dashboard Cite

Glycosylation is an important and common form of posttranscriptional modification of proteins in cells. A vast array of biological functions has been ascribed to glycans during the last decade thanks to a rapid evolution in glycomic technologies. Glycogenes highly expressed at the human ocular surface include families of glycosyltransferases, proteoglycans, glycan degradation proteins, as well as mucins and carbohydrate-binding proteins such as the galectins. On the apical glycocalyx, mucin O-glycans promote boundary lubrication, prevent bacterial adhesion and endocytic activity, and maintain epithelial barrier function through interactions with galectins. The emerging roles attributed to glycans are contributing to the appreciation of their biological capabilities at the ocular surface.

show abstract

Antiadhesive Character of Mucin O-glycans at the Apical Surface of Corneal Epithelial Cells

Abstract: These results indicate that epithelial O-glycans contribute to the antiadhesive properties of cell surface-associated mucins in corneal epithelial cells and suggest that alterations in mucin O-glycosylation are involved in the pathology of drying mucosal diseases (e.g., dry eye).

Cited by 64 publications

References 33 publications

CA125 is a potential biomarker to predict surgically incurable gastric and cardia cancer

CA125 is a potential biomarker to predict surgically incurable gastric and cardia cancer

Clinical staining of the ocular surface: Mechanisms and interpretations

Glycobiology of the ocular surface: mucins and lectins

Contact Info

Product

Resources

About