Glycobiology of the ocular surface: mucins and lectins

Argüeso, Pablo

doi:10.1007/s10384-012-0228-2

Cited by 54 publications

(55 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[15][16][17] The mucins involved in this lattice barrier are considered to comprise MUC1 and MUC16, which are membrane-associated mucins.…”

Section: Discussionmentioning

confidence: 99%

“…[15][16][17] As the X-ray crystallographic structure of human Gal-2 revealed that the two binding sites of the homodimer are located at opposite ends of the dimer, 18) allowing sufficient room to bind to larger molecules, it is possible that Gal-2, which is abundant in gastric mucous cells, may similarly potentially form a lattice 19) with secreted mucins or crosslink cell surface mucins to strengthen the mucus barrier and contribute to the protection of the stomach.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Identification of Galectin-2–Mucin Interaction and Possible Formation of a High Molecular Weight Lattice

Tamura

Sato

Nakajima

et al. 2017

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Galectins comprise a group of animal lectins characterized by their specificity for β-galactosides. Galectin-2 (Gal-2) is predominantly expressed in the gastrointestinal tract and has been identified as one of the main gastric mucosal proteins that are proposed to have a protective role in the stomach. As Gal-2 is known to form homodimers in solution, this may result in crosslinking of macromolecules with the sugar structures recognized by Gal-2. In this study, we report that Gal-2 could interact with mucin, an important component of gastric mucosa, in a β-galactoside-dependent manner. Furthermore, Gal-2 and mucin could form an insoluble precipitate, potentially through the crosslinking of mucins via Gal-2 and the formation of a lattice, resulting in a large insoluble complex. Therefore, we suggest that Gal-2 plays a role in the gastric mucosa by strengthening the barrier structure through crosslinking the mucins on the mucosal surface.

show abstract

“…[15][16][17] The mucins involved in this lattice barrier are considered to comprise MUC1 and MUC16, which are membrane-associated mucins.…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Identification of Galectin-2–Mucin Interaction and Possible Formation of a High Molecular Weight Lattice

Tamura

Sato

Nakajima

et al. 2017

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…However, in general, the membrane-spanning mucins play critical roles in the protection of the ocular surface. The O -glycans on membrane spanning mucins provide a rigid structure (so called bottle brush) allowing these mucins to extend 200-500 nm above (Argueso, 2013; Bramwell et al, 1986; Komatsu et al, 1997) the ocular surface. As the O -glycans are hydrophilic they bind water which helps to maintain their rigid structure (Argueso, 2013; Gipson, 2004) .…”

Section: Membrane Spanning Mucinsmentioning

confidence: 99%

“…Thus it is thought that one function of these mucins is to maintain the hydration of the ocular surface. A second function of membrane-bound mucins is to provide lubrication and anti-adhesive properties between the cells of the ocular surface and conjunctiva during the blink (Argueso, 2013; Gipson, 2004). A third function is to contribute to the epithelial barrier.…”

Section: Membrane Spanning Mucinsmentioning

confidence: 99%

Tear film mucins: Front line defenders of the ocular surface; comparison with airway and gastrointestinal tract mucins

Hodges

Dartt

2013

Experimental Eye Research

158

103

View full text Add to dashboard Cite

The ocular surface including the cornea and conjunctiva and its overlying tear film are the first tissues of the eye to interact with the external environment. The tear film is complex containing multiple layers secreted by different glands and tissues. Each layer contains specific molecules and proteins that not only maintain the health of the cells on the ocular surface by providing nourishment and removal of waste products but also protect these cells from environment. A major protective mechanism that the corneal and conjunctival cells have developed is secretion of the innermost layer of the tear film, the mucous layer. Both the cornea and conjunctiva express membrane spanning mucins, whereas the conjunctiva also produces soluble mucins. The mucins present in the tear film serve to maintain the hydration of the ocular surface and to provide lubrication and anti-adhesive properties between the cells of the ocular surface and conjunctiva during the blink. A third function is to contribute to the epithelial barrier to prevent pathogens from binding to the ocular surface. This review will focus on the different types of mucins produced by the corneal and conjunctival epithelia. Also included in this review will be a presentation of the structure of mucins, regulation of mucin production, role of mucins in ocular surface diseases, and the differences in mucin production by the ocular surface, airways and gastrointestinal tract.

show abstract

“…They are synthesized by the transcription of the genes called glycogens. However, they have neither enzyme activity nor antibody properties (8). The interactions of carbohydrates and their specific lectins are involved in various physiologic processes like intercellular interactions, signal transduction, intracellular protein transport, fertilization, cellular adhesions, interferon and interleukin synthesis, infections, embryogenesis, cell hyperplasia and dysplasia, carcinogenesis, and metastasis (9,10).…”

mentioning

confidence: 99%

Selenium Protects Retinal Cells from Cisplatin-Induced Alterations in Carbohydrate Residues

Akşit¹,

Yazıcı²,

Akşit³

et al. 2016

Balkan Med J

View full text Add to dashboard Cite

Background: Investigate alterations in the expression and localization of carbohydrate units in rat retinal cells exposed to cisplatin toxicity. Aims: The aim of the study was to evaluate putative protective effects of selenium on retinal cells subjected to cisplatin. Study Design: Animal experiment. Methods: Eighteen healthy Wistar rats were divided into three equal groups: 1. Control, 2. Cisplatin and 3. Cisplatin+selenium groups. After anesthesia, the right eye of each rat was enucleated. Results: Histochemically, retinal cells of control groups reacted with α-2,3-bound sialic acid-specific Maackia amurensis lectin (MAA) strongly, while cisplatin reduced the staining intensity for MAA. However, selenium administration alleviated the reducing effect of cisplatin on the binding sites for MAA in retinal cells. The staining intensity for N-acetylgalactosamine (GalNAc residues) specific Griffonia simplicifolia-1 (GSL-1) was relatively slight in control animals and cisplatin reduced this slight staining for GSL-1 further. Selenium administration mitigated the reducing effect of cisplatin on the binding sites for GSL-1. A diffuse staining for N-acetylglucosamine (GlcNAc) specific wheat germ agglutinin (WGA) was observed throughout the retina of the control animals. In particular, cells localized in the inner plexiform and photoreceptor layers are reacted strongly with WGA. Compared to the control animals, binding sites for WGA in the retina of rats given cisplatin were remarkably decreased. However, the retinal cells of rats given selenium reacted strongly with WGA. Conclusion: Cisplatin reduces α-2,3-bound sialic acid, GlcNAc and GalNAc residues in certain retinal cells. However, selenium alleviates the reducing effect of cisplatin on carbohydrate residues in retinal cells.

show abstract

Glycobiology of the ocular surface: mucins and lectins

Cited by 54 publications

References 42 publications

Identification of Galectin-2–Mucin Interaction and Possible Formation of a High Molecular Weight Lattice

Identification of Galectin-2–Mucin Interaction and Possible Formation of a High Molecular Weight Lattice

Tear film mucins: Front line defenders of the ocular surface; comparison with airway and gastrointestinal tract mucins

Selenium Protects Retinal Cells from Cisplatin-Induced Alterations in Carbohydrate Residues

Contact Info

Product

Resources

About