Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant

Lemus, David; Dabancens, Alfredo; Illanes, Julio; Fuenzalida, Marcela; Guerrero, Antonio José Moreno; López, Claudia

doi:10.4067/s0716-97602001000300010

Cited by 12 publications

(13 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The antiangiogenic effect of some corticosteroids in combination with heparine has been well established (Folkman 1989). A direct antiangiogenic effect of betamethasone has recently been reported (Lemus et al, 2001) and it is confirmed in the present study.…”

Section: Discussionsupporting

confidence: 78%

“…Significant increases in the dose of steroidal drugs may bring about additional therapeutic benefits, mediated by membrane-bound receptors and/or physicochemical interactions with the cell membrane (Buttgereit et al, 1998). The fact that the concentration of betamethasone needed in the present study to obtain a minimum antiangiogenic effect was in the range of 2x10 -7 moles/litre (Lemus et al, 2001), seems to indicate that its effect may most likely be genomically mediated.…”

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Effects of Steroidal and Non Steroidal Drugs on the Neovascularization Response Induced by Tumoral TA3 Supernatant on CAM from Chick Embryo

Zúñiga¹,

Fuenzalida²,

Guerrero³

et al. 2003

Biol. Res.

Self Cite

View full text Add to dashboard Cite

Angiogenesis, the development of new blood vessels from the existing vascular network, may result as a consequence of the increase or decrease of proangiogenic or antiangiogenic factors, respectively. The tumor itself could up-regulate the production of angiogenic factors. Recently, we established that the steroidal drug betamethasone in low concentration inhibit the neovascularization promoted by TA3 Ts on CAM of chick embryos. We describe here the effects of the non-steroidal drug ketoprofen, alone or in association with betamethasone, on the angiogenesis promoted by TA3 Ts on CAM. The main finding reported here is that the formation of new blood vessels is strongly inhibited by low concentrations of ketoprofen. The association of both drugs produced a synergistic effect, significantly decreasing tumoral supernatant angiogenesis. It is known that steroidal anti-inflammatory drugs inhibit the enzymes required for the production of prostaglandins through a nuclear GR mediated mechanism. This may operate as a general mechanism in endothelial cells as well. Considering that the induction of COX 1 and COX2 are inhibited by ketoprofen, and that these enzymes are located in the stromal compartment of the CAM, we propose that its antiangiogenic effect may occur via inhibition of the two COX isoforms. In fact, we found that ketoprofen induced apoptosis in both the stromal fibroblast and endotelial cells. The potentiated effect of the combination of betamethasone and ketoprofen may have some therapeutic projections in the control of pathological angiogenesis.

show abstract

Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 96%

Effects of Steroidal and Non Steroidal Drugs on the Neovascularization Response Induced by Tumoral TA3 Supernatant on CAM from Chick Embryo

Zúñiga¹,

Fuenzalida²,

Guerrero³

et al. 2003

Biol. Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previously, we have shown that 10 -9 M of Betamethasone (β-met) (minimal antiangiogenic concentration) mediated a considerable inhibition of neovascularization promoted by murine A/J TA 3 tumor supernatant on the chorioallantoic membrane (CAM) of 12-day chick embryos (Lemus et al, 2001). On the other hand, the association of β-met with ketoprofen produced a synergistic effect, significantly decreasing tumor angiogenesis on CAM (Zúñiga et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Betamethasone inhibits tumor development, microvessel density and prolongs survival in mice with a multiresistant adenocarcinoma TA3

et al. 2010

Self Cite

View full text Add to dashboard Cite

Tumor resistance to traditional cancer treatments poses an important challenge to modern science. Thus, angiogenesis inhibition is an important emerging cancer treatment. Many drugs are tested and corticosteroids have shown interesting results. Herein we investigate the effect on microvessel density, survival time and tumoral volume of mice with TA3-MTX-R tumors. Twenty six mice were inoculated with 1x10 6 tumor cells, 4-5 days after injection, six mice were injected with PBS (group A) and twenty mice were treated with β-met (group B). All animals from Group A died on day 22. Group B was divided into B1 (treated discontinued) and B2 (treated daily) and observed until day 88. All mice were processed for histo-immunohistochemical analysis and the blood vessels were counted. A decrease in microvessel density and tumoral volume and longer survival times were observed in the treated group. We propose that the antiangiogenic β-met effect explains, at least partially, its tumor inhibitory properties. As an important perspective, we will experimentally combine these strategies with those recently described by us with regard to the important antiangiogenic-antitumor effects of Trypanosoma cruzi calreticulin. Since the molecular targets of these strategies are most likely different, additive or synergic effects are envisaged.

show abstract

“…This method, introduced by Rous andMurphy in 1911 (Rous andMurphy, 1911), makes use of the high capacity of the chorioallantoic mesenchyma for responding to the presence of exogenous tissue with proliferation (Coulombre, 1967, Hamburger, 1960 and neoangiogenesis (Lemus et al, 2001), which favors a fast incorporation and vascularization of the grafts. In a previous work, it was described that carotid bodies and nodose ganglia from young adult rats were incorporated into the chorioallantoic membrane, vascularized, and maintained alive for up to 12 days, showing preservation of their general morphological characteristics (Gual et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Electrophysiological properties of rat nodose ganglion neurons co-transplanted with carotid bodies into the chick chorioallantoic membrane

Eugenı́n

Eyzaguirre

2005

Biol. Res.

View full text Add to dashboard Cite

The electrophysiological properties of nodose ganglion neurons were evaluated immediately after removing nodose ganglia from young adult rats and 3 to 10 days after nodose ganglia implantation -either alone or coimplanted with carotid bodies-onto the chick chorioallantoic membrane. Implanted and co-implanted nodose neurons were less excitable than acutely recorded nodose neurons. Co-implanted neurons also showed reduced amplitudes for both action potentials and spike after-hyperpolarizations relative to those found in acutely recorded nodose ganglion neurons and a smaller time constant (τ) than that found in implanted neurons. In addition, no spontaneous activity was recorded from nodose ganglion neurons co-implanted with carotid bodies during 3-9 days, which suggests that functional synapses between carotid glomus cells and nodose neurons were not yet established. Results indicate the feasibility of obtaining viable nodose neurons for up to 10 days grafted onto the chick chorioallantoic membrane, where they can conserve most of their passive and active membrane properties and also are susceptible to carotid bodies trophic influences. They also suggest that nodose neurons would need more time for the development of functional synapses when grafted with carotid body glomus cells.

show abstract

Antiangiogenic effect of betamethasone on the chick cam stimulated by TA3 tumor supernatant

Cited by 12 publications

References 0 publications

Effects of Steroidal and Non Steroidal Drugs on the Neovascularization Response Induced by Tumoral TA3 Supernatant on CAM from Chick Embryo

Effects of Steroidal and Non Steroidal Drugs on the Neovascularization Response Induced by Tumoral TA3 Supernatant on CAM from Chick Embryo

Betamethasone inhibits tumor development, microvessel density and prolongs survival in mice with a multiresistant adenocarcinoma TA3

Electrophysiological properties of rat nodose ganglion neurons co-transplanted with carotid bodies into the chick chorioallantoic membrane

Contact Info

Product

Resources

About