Antiangiogenic properties of Koetjapic acid, a natural triterpene isolated from Sandoricum koetjaoe Merr

Abstract

BackgroundAngiogenesis, the formation of new blood vessels, has become an important target in cancer therapy. Angiogenesis plays an important role in tumor growth and metastasis. Koetjapic acid (KA) is a seco-A-ring oleanene triterpene isolated from S. koetjape. The solvent extract of this plant species was shown previously to have strong antiangiogenic activity; however the active ingredient(s) that conferred the biological activity and the mode of action was not established. Given the high concentration of KA in S. koetjape, an attempt has been made in this study to investigate the antiangiogenic properties of KA.ResultsTreatment with 10-50 μg/ml KA resulted in dose dependent inhibition of new blood vessels growth in ex vivo rat aortic ring assay. KA was found to be non-cytotoxic against HUVECs with IC50 40.97 ± 0.37 μg/ml. KA inhibited major angiogenesis process steps, endothelial cell migration and differentiation as well as VEGF expression.ConclusionsThe non-cytotoxic compound, KA, may be a potent antiangiogenic agent; its activity may be attributed to inhibition of endothelial cells migration and differentiation as well VEGF suppression.

<i>In Vitro</i> Antimetastatic Activity of Koetjapic Acid against Breast Cancer Cells

“…[12][13][14] Since the n-hexane extract showed potential anti-proliferative activity against a breast cancer cell line, 15) it has been suggested that KA may have potential value as an anti-breast cancer agent. Accordingly, this study has been performed in order to examine the growth inhibitory effect of KA on human breast cancer cells (MCF 7), and to elucidate the mechanism(s) involved in the action of KA.…”

Use of Nigella sativa Linn. Supercritical Carbon Dioxide Extract for Targeting the Angiogenesis Cascade

“…These assays provide a valuable data for anti-angiogenesis assessment [37,42]. Endothelial cells tend to be in contact with their neighbouring cells.…”

“…Inhibitory effect of A3 and B3 was investigated in vivo using fertilized chicken embryo [37]. Fertile eggs were cleaned and kept horizontally with rotation for 3-5 days at 37°C in a humidified incubator to ensure formation of chorioallantoic membrane (CAM) vessel network.…”

Anti-angiogenic quassinoid-rich fraction from Eurycoma longifolia modulates endothelial cell function

“…TAF273, TAF355 and TAF401 were added at final concentrations of 6.25, 12.5, 25, 50 and 100 μg/ml and the median inhibitory concentrations (IC 50 ) were determined by nonlinear regression analysis of log-concentration-response curves. Similarly, serial concentrations (6.25 to 100 μg/ml) of suramin were used as standard reference (Nassar et al, 2011), whereas distilled water was used as negative control. On day four, the medium was replaced with a fresh one containing the test materials.…”

“…Anti-neovascularization effect of TAF273 was investigated in vivo in chorioallantoic membrane (CAM) assay using chicken embryos as previously reported (Nassar et al, 2011). Five day-old fertilized eggs were obtained from local hatchery.…”