Antiarthritic activity of a polyherbal formulation against Freund′s complete adjuvant induced arthritis in Female Wistar rats

Abstract

Objectives:To formulate a polyherbal formulation and evaluate its antiarthritic activity against Freund's complete adjuvant induced arthritis in Female Wistar rats.Materials and Methods:Glycosmis pentaphylla, Tridax procumbens, and Mangifera indica are well-known plants available throughout India and they are commonly used for the treatment of various diseases including arthritis. The polyherbal formulation was formulated using the ethanol extracts of the stem bark of G. pentaphylla, whole plant of T. procumbens, and leaves of M. indica. The polyherbal formulation contains the ethanol extracts of G. pentaphylla, T. procumbens, and M. indica in the ratio of 2:2:1. The quality of the finished product was evaluated as per the World Health Organization's guidelines for the quality control of herbal materials. Arthritis was induced in female Wistar rats using Freund's complete adjuvant (FCA), and the antiarthritic effect of polyherbal formulation was studied at doses of 250 and 500 mg/kg. The effects were compared with those of indomethacin (10 mg/kg). At the end of the study, blood samples were collected for biochemical and hematological analysis. The radiological examination was carried out before terminating the study.Results:Polyherbal formulation showed significant antiarthritic activity at 250 and 500 mg/kg, respectively, and this effect was comparable with that of indomethacin. The antiarthritic activity of polyherbal formulation is supported by biochemical and hematological analysis.Conclusion:The polyherbal formulation showed signinicant antiarthritic activity against FCA-induced arthritis in female Wistar rats.

Suppression of calpain expression by NSAIDs is associated with inhibition of cell migration in rat duodenum

“…Indomethacin is a non-selective inhibitor of COX enzymes that has a high ulcerogenic potential and causes ulcers in drug-treated animals at the doses used in this study (Kato et al 2001; Peskar et al 2001; Tavares 2000), whereas NS-398 is a COX 2-selective inhibitor (coxib) that does not cause ulceration in drug treated animals, but inhibits ulcer healing once they are induced (Brzozowski et al 2001; Kato et al 2001; Peskar et al 2001; Tavares 2000; Tomisato et al 2004). Animals were treated with indomethacin and NS-398 at a dose of 10 mg/kg/day in this study, which is consistent with the experimental model of indomethacin-induced GI damage that is commonly used (Ishida et al 2013; Kato et al 2009; Lim et al 2012; Rohde et al 2015; Satoh and Urushidani 2016) as well as their experimental use by other research groups in studying the anti-nociceptive effects of NSAIDs (Brzozowski et al 2001; Euchenhofer et al 1998; Lichtenberger et al 2015; Peskar et al 2001; Petchi et al 2015; Pozzoli et al 2007). It will be interesting to evaluate the effects of a greater range of NSAIDs for their effects on cell migration in duodenal epithelia and in the more distant epithelia of the jejunum and ileum in future experiments to determine if there is a correlation between inhibition of calpain protease expression and inhibition of migration following NSAID treatment there.…”

β-Sitosterol-loaded solid lipid nanoparticles ameliorate complete Freund’s adjuvant-induced arthritis in rats: involvement of NF-кB and HO-1/Nrf-2 pathway

“…The exact initiation mechanism of arthritis is still not fully clear, but some researcher and previous publish report suggest that the various pro-inflammatory cytokines, immune cell populations and inflammatory mediators is admitted. During the pathological process and early symptoms of swelling, heat, reduced joint function and pain; the later stage exhibit different degrees of deformity and joint stiffness accompanying bone damage and disability risk (Woodruff et al., 2002 ; Funk et al., 2006 ; Petchi et al., 2015 ). Research suggests that inflammation is a physiological reaction against harmful stimuli, such as pathogens in the body.…”

Biochemical Alterations in the Liver and Kidney of Rats Following Sub-acute Administration of Aqueous Extract of Stem-bark of Anacardium occidentale (Cashew Tree)

“…It has been reported in the earlier studies that tannins toxicity may affect membranes, alter the excretion of cations, increases the excretion of proteins and essential amino acids in man and other monogastric animals [32,33] constellation of these toxic effects of tannins may be contributory to our observation of an increase in the levels of urea and creatinine in kidney tissue. disorders, the scientific basis for such applications are not completely established [31]. The phytochemical screening of methanol extract bark of cashew tree revealed the nt amount of tannins and moderate amount of saponins to suggest evidence of tannins toxicity in the sub-chronic to Anacardium occidentale stembark in complementary and alternative medicine .…”