Antibacterial activities of Anisomeles indica constituents and their inhibition effect on Helicobacter pylori-induced inflammation in human gastric epithelial cells

Bioevaluation of Anisomeles indica extracts and their inhibitory effects on Helicobacter pylori-mediated inflammation

“…Previously, it has been reported that extractions of Anisomeles indica inhibited Helicobacter pylori growth and Helicobacter pyloriinduced inflammation (Hsieh et al, 2008). Based on our recent study, OVT-one of the pure constituents isolated from Anisomeles indica-may be responsible for the antimicrobial activity and effects against Helicobacter pylori-infection (Rao et al, 2012). This study further suggested that it would be worth validating the molecular mechanisms by which Anisomeles indica acts against Helicobacter pylori-induced inflammation either in the gastric epithelium or macrophages, and to develop more efficient and feasible approaches for production of OVT-containing extracts from Anisomeles indica.…”

“…It has been previously reported that extractions and isolated constituents of Anisomeles indica exhibit inhibition of inflammatory mediators and tumor cell proliferation (Hsieh et al, 2008;Rao et al, 2009). Additionally, ovatodiolide (OVT) as a pure constituent isolated from Anisomeles indica has been shown to have bactericide activity against Helicobacter pylori, resulting in reduction of Helicobacter pylori bacterial adhesion and invasion to human gastric epithelial (AGS) cells in our recent study (Rao et al, 2012). For economical purification of the effective constituent, we developed ethanol extracts containing OVT and examined their bactericidal function and attenuation of inflammatory responses in Helicobacter pyloriinfected cells.…”

“…OVT from Anisomeles indica was prepared as described previously (Rao et al, 2012). The active compound, OVT contained in the various extracts was confirmed by high-performance liquid chromatography (HPLC) [column:…”

“…Our recent study showed that the pure constituent OVT isolated from Anisomeles indica was able to inhibit Helicobacter pylori-induced inflammation in human gastric epithelial cells (Rao et al, 2012). To further manufacture large amounts of OVTcontaining extracts, various concentrations of ethanol for extraction of Anisomeles indica were performed and the content of OVT was analyzed by HPLC ( Supplemental Fig.…”

Toxicity Evaluation of Anisomeles indica Kuntze Leaf Flavonoid Fraction

“…The plant is used for the treatment of inflammatory skin diseases, liver disease and protection, gastrointestinal disease, hypertension and immune system deficiencies [34][35]. Extracts and isolated constituents of A. indica has been demonstrated to possess, anti-inflammatory, anti-tumor cell proliferation, bactericidal function against H. pylori [21,22,36]. Despite the medicinal and pharmacological properties of A. indica extracts and isolated constituents, there are no data available in the pertinent literature on the toxicological concerns of this plant, its extracts and isolated constituents that would be useful for the safety assessment and dosage fixation.…”