Antibacterial activities of Curcuma mangga Val. extract in some solvents to Staphylococcus aureus and Escherichia coli

Muchtaromah, Bayyinatul; Safitri, Evika Sandi; Fitriasari, Prilya Dewi; Istiwandhani, Jujuk

doi:10.1063/5.0002490

Cited by 10 publications

(8 citation statements)

References 7 publications

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“…gingivalis is a Gram-negative bacterium has a complex structure, there are endotoxins in the form of lipopolysaccharides in its walls and the outer membrane surrounds the plasma membrane has peptidoglycan in it. Sensitivity to incoming antibacterial compounds may be hindered due to the complex bacterial cell structure in P. gingivalis [46]. This is in line with previous research which shows that Grampositive bacteria have a larger inhibitory zone compared to Gram-negative bacteria [47].…”

Section: Figuresupporting

confidence: 87%

Antibacterial Activity of Robusta Coffee (Coffea Canephora) Husk Extract Against Enterococcus Faecalis and Phorphyromonas Gingivalis: In Vitro Study

Prasetya,

Fatimatuzzahro,

Ermawati

et al. 2024

Trends Sci

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Enterococcus faecalis is one of the bacteria that is commonly found in infected root canals, necrotic pulp and failure of root canal treatment, while Phorphyromonas gingivalis play a role in inducing periodontitis. Both bacteria can enter the blood circulation and lead to risk factors for systemic disease such as atherosclerosis. ChKM is the most widely used root canal sterilization, and also 0.2 % chlorhexidine mouthwash for long-term usage. It can destroy a potent cell and is allergenic. Developing an alternative material for root canal sterilization and mouthwash for plaque control is necessary. One of the alternative natural products that is predicted to have antibacterial effect is robusta coffee husk peels. This research aimed to investigate the antibacterial activity of robusta coffee (Coffea canephora) husk extract on E. faecalis and P. gingivalis. The robusta coffee husk was extracted using the maceration process with 96 % ethanol as the solvent. This research is an experimental laboratory, was tested by disk diffusion methods (Kirby-Baurer) using Gram-positive E. faecalis ATCC 29212 and Gram-negative P. gingivalis ATCC 33277 consisted of 6 groups: Aquadest as a negative control group, ChKM (for E.facecalis) and 0.2 % chlorhexidine (for P. gingivalis) as positive control, 4 treatment groups of coffee robusta husk extract with different concentrations there were 250, 250, 500, 750 and 1,000 mg/mL. The antibacterial effect was examined by measuring the clear zone around the disk paper. The results of this study proved the presence of antibacterial activity of coffee robusta husk extract against E. faecalis and P. gingivalis. The higher concentrations followed with a greater antibacterial effect. HIGHLIGHTS Robusta coffee husk extract (Coffea canephora) has an antibacterial property against Gram-positive and Gram-negative bacteria Robusta coffee husk was extracted using the maceration process, and disk diffusion methods (Kirby-Baurer) for antibacterial test Robusta coffee husk extract with a concentration of 1,000 mg/mL showed the greatest inhibition zone. The higher the concentration, the bigger inhibition zone appeared GRAPHICAL ABSTRACT

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Section: Figuresupporting

confidence: 87%

Antibacterial Activity of Robusta Coffee (Coffea Canephora) Husk Extract Against Enterococcus Faecalis and Phorphyromonas Gingivalis: In Vitro Study

Prasetya,

Fatimatuzzahro,

Ermawati

et al. 2024

Trends Sci

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“…Particularly, S. aureus is considered as one of the most sensitive bacterial strains that could easily be killed by various natural or synthetic drugs (Thilza et al, 2010; Farjan et al, 2014). On the contrary side, E. coli and K. pneumonia have been considered as the most resistant bacterial strains which require high potency of drugs or combination therapy for complete eradication (Muchtaromah et al, 2020; Mostafa et al, 2018). Based on current findings we believe that O. japonicum is an important medicinal plant, to elaborate its clinical importance extensive studies involving cell lines and animal models are required.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical and antibacterial analyses of Onychium japonicum (Thunb.) Kunze

Shah

Hassan

Alsanie

et al. 2022

Preprint

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In clinical practice bacterial resistance against antibiotics has become a serious health problem, thus using alternative approaches such as natural products as the supplementary drug could solve it. Therefore, the current study was conducted to thoroughly investigate the enrichment of phytochemicals and antibacterial potential of O. japonicum. The fronds samples of O. japonicum prepared in different solvents were used against MDR bacterial strains and phytochemical analyses. The analyses of data revealed that O. japonicum was enriched with flavonoids, alkaloids, saponins, tannins, glycosides, carotenoids, terpenoids, phlobatanins, phenols, and coumarins while quantitatively this plant has a significantly higher content of phenols(1100.91µM/g) followed by sugar contents (748.67µM/ml), ascorbic acid (426.12mM/g), flavonoids (160.65mg/g), anthocyanin (101.06µM/g) and proline (80.58µM/g). On the other hand, the organic extracts of O. japonicum were highly active against all bacterial strains while hydric extract was inactive against selected bacteria. Specifically, O. japonicum was highly active against S. aureus in all organic extracts (chloroform=16.66±0.33, ethyl acetate=15±0.57, methyl alcohol= 14±0.57, N-hexane=20.33±0.33) followed by K. pneumonia (chloroform=14.33±0.33, ethyl acetate=4.33±4.33, methyl alcohol=3.66±3.66, N-hexane=16.66±0.33) and P. aeruginosa (chloroform= 8.33±4.17, ethyl acetate=8.33±4.17, methyl alcohol=6±3.00, N-hexane=9.33±4.66), while E. coli (chloroform=0±0.00, ethyl acetate=7±3.51, methyl alcohol=3.33±3.33, N-hexane=4±4.00). Based on current findings it is concluded that O. japonicum is enriched in many useful phytochemicals that could be use as a supplement with other traditionally used antibiotics.

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“…For this technique, DMSO 10% and gentamicin (10 µg) were used as negative and positive controls, respectively. The equation below was used to calculate the IZ diameter ( Muchtaromah et al, 2020 ):

…”

Section: Methodsmentioning

confidence: 99%

Nanoemulsion and nanoencapsulation of a hydroethanolic extract of Nettle (Urtica dioica) and Wormwood (Artemisia absinthium): comparison of antibacterial and anticancer activity

Rahmani,

Karimi,

Saffari

et al. 2024

Front. Chem.

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Introduction: Nanoemulsion and nanoencapsulation are attractive novel methods that can be used for incorporating active plant extracts in food preparations and pharmaceutical formulations. In the current study, we aimed to investigate the anticancer and antibacterial effects of hydroethanolic extracts of Nettle (NE), Wormwood (WE), and the combination of the two plants (CNWE), as well as their nanoemulsion forms (NN, NW, CNNW) and nanoencapsulation forms (CN, CW, and CCNW).Methods: The morphology and structure of the nanoemulsion and nanoencapsulation preparations were assessed utilizing dynamic light scattering (DLS) along with transmission electron microscopy (TEM). The antibacterial activity of the prepared formulations were assessed by determining minimum inhibitory concentration (MIC), zone of inhibition diameter, minimum bactericidal concentration (MBC), along with biofilm growth inhibition against Salmonaella typhimurium and Klebsiella. pneumoniae. The anticancer activity was evaluated via a MTT assay in the colon cancer cell line (HCT116).Results: The nanoemulsion and nanoencapsulation particle size varied between 10 and 50 nm and 60 and 110 nm, respectively. The MIC values were between 11.25 and 95 µg/mL along with MBC values between 11.25 and 190 µg/mL. The highest inhibition of biofilm formation was observed with CCNW against K. pneumoniae (∼78.5%) and S. typhimurium (∼73%). In descending order, the inhibition of biofilm formation was CCNW > CW > CN > CNNW > NN > NW > CNWE > NE > WE against the tested bacteria. The IC50 values for NE, WE, CNWE, NN, NW, CNNW, CN, CW, and CCNW were determined as 250, 170, 560, 380, 312, 370, 250, 420, and 700 µg/mL, respectively. Exposure to a high concentration of NW resulted in a significantly lower HCT116 viability compared to other groups. Taken together, CNNW, and CCNW showed the highest antibacterial and anticancer activitiy.Discussion: Nanoemulsion and nanoencapsulation were effective ways to increase the antibacterial and anticancer activity of the extracts and could be used in the food and pharmaceutical industries.

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Antibacterial activities of Curcuma mangga Val. extract in some solvents to Staphylococcus aureus and Escherichia coli

Cited by 10 publications

References 7 publications

Antibacterial Activity of Robusta Coffee (Coffea Canephora) Husk Extract Against Enterococcus Faecalis and Phorphyromonas Gingivalis: In Vitro Study

Antibacterial Activity of Robusta Coffee (Coffea Canephora) Husk Extract Against Enterococcus Faecalis and Phorphyromonas Gingivalis: In Vitro Study

Phytochemical and antibacterial analyses of Onychium japonicum (Thunb.) Kunze

Nanoemulsion and nanoencapsulation of a hydroethanolic extract of Nettle (Urtica dioica) and Wormwood (Artemisia absinthium): comparison of antibacterial and anticancer activity

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