The endophytes are microorganisms that colonize plant tissue without causing infections. One of endophytic microorganisms, fungi, comprises a reliable source of genetic diversity and has been applied in pharmacology. In this study, the endophytic fungi was isolated from fruit and seed of Syzygium cumini and identified based on ITS-rDNA. Five fungal isolates were obtained from fruit and seed of Jambolana, two isolates from fruit and three isolates from seed. Based on ITS-rDNA analysis showed that that endophytic fungus isolates from jambolana (S. cumini L.) Skeels was identified as Neofusicoccum parvum (F1 isolates), Pestalotiopsis vismiae (F2 isolates), Phomopsis sp. (S1 and S3 isolates), Colletotrichum fructicola (S2 isolates). All the isolates are belonged to Ascomycota.

Talangagung landfill, Malang Regency has used the controlled landfill method. The landfill process produces methane gas that has been flowed as fuel so that it is estimated that there are bacteria with a variety of enzymatic activities that have worked in degrading waste. The purpose of this study was to isolate bacteria from landfill and determine the ability to produce amylase, protease, lipase, and cellulase and test the compatibility of bacteria. The methods used include bacterial isolation using the pour plate method and purification of bacterial isolates using the streak plate method. Bacterial selection is done on selective media containing starch, tributyrin, cellulose, and skim milk. There were 21 bacterial isolates successfully isolated 13 consisting of bacterial isolates showed proteolytic activity, 10 cellulolytic bacteria, 8 amylolytic, and 15 lipolytic. The isolates that show compatible results are BTA 5.7.14, BTA 5.7.7, BTA 5.7.12, and BTA 5.7.6 therefore they can be used as a potential consortium for organic waste recycle.

Curcuma mangga is one of the traditional medicines that have the effect dealing with infertility problems. Staphylococcus aureus and Escherichia coli are bacteria often found as the cause of the female reproductive tract infection. This study aimed to determine the antibacterial activities of several solvents of C. mangga extract against S.aureus and E.coli. C. mangga rhizome was extracted by a maceration method using ethanol (polar), chloroform (semi-polar), and nhexane (non-polar) solvents. The antibacterial activity test against S.aureus and E.coli using Kirby Bauer with the extract various concentrations of (%) 50, 25,12.5, 6.25, 3.13, 1.56, 0.78, and 0.39. The highest inhibition zone diameter for S.aureus was obtained by ethanol (10.11 m), chloroform (9.21 mm), n-hexane (6.05 mm) extract while for E. coli , the highest inhibition zone diameter was respectively achieved by ethanol extract (8.06 mm), n-hexane (5.88 mm) and, chloroform (4.19 mm). The MIC value of ethanol extract to S. aureus was found at concentrations of 3.13% and MBC at 6.25%. The MIC value of ethanol extract on E. coli was attained at concentration of 6.25% and MBC at 12.50%. Ethanol extract of C. mangga rhizome produced the most antibacterial activities than chloroform and n-hexane.

TRAINING ON MAKING NATA DE COCO FOR PKK ORGANISATION IN TASIKMADU, LOWOKWARU, MALANG CITY. One of the functional food products is nata. Nata is a collection of cellulose which has a chewy texture, white, producing gel sheets that float on the liquid surface. Material that is often used as a medium for making nata is coconut water, known as Nata de Coco. Acetobacter xylinum bacteria are added in the process of making nata de coco on steril condition. The process is important to be informed to the community, in particular the PKK organization through community development program. The methods used include lectures, practice and evaluation. Survey of partners showed that 23,8% had known the process of making nata de coco while 76,2% had not known the process, through this training, 68% partners strongly agree nata production is used as a business idea.

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