Identification of Endophytic Fungi from Fruits and Seeds of Jambolana (Syzygium cumini L.) Skeels

Hanin, Nada Asmara; Fitriasari, Prilya Dewi

doi:10.1088/1755-1315/276/1/012060

Cited by 12 publications

(10 citation statements)

References 17 publications

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“…Eight endophytic fungi isolated from the stem bark of S. jambos have been found, namely Spegazzinia, Auxarthronopsis, Pectinotrichum, Phaeotrichosphaeria, Poaceascoma, Fusarium, Thielaviopsis, and Nigrospora. Seven endophytic fungi have not been found in other Syzygium genera, except Fusarium which is also found in S. cumini (Hanin and Fitriasari 2019). Based on the findings of endophytic fungi in this research and literature study from plants of the same genus, there are similarities in the diversity of endophytic fungi.…”

Section: Discussionsupporting

confidence: 45%

Antibacterial activity of endophytic fungi isolated from the stem bark of jambu mawar (Syzygium jambos)

et al. 2022

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Abstract. ‘Aini K, Elfita, Widyajanti H, Setiawan A, Kurniawati AR. 2021. Antibacterial activity of endophytic fungi isolated from the stem bark of jambu mawar (Syzygium jambos). Biodiversitas 23: 521-532. Syzygium jambos (jambu mawar) is widely used in South Sumatra as a traditional medicine to treat various diseases, including pathogenic bacterial infections. Literature studies report that parts of S. jambos have been used worldwide for treating diarrhea accompanied by fever, dysentery, sore throat, diabetes, and other infectious diseases. Endophytic fungi isolated from medicinal plants have high diversity, and the biological activity of their secondary metabolites is associated with their host. Therefore, this study aimed to determine the diversity of endophytic fungi from the stem bark of S. jambos and the antibacterial activity of secondary metabolites of endophytic fungi. Fungal isolates with high antibacterial activity were identified to obtain fungal species, and the structure of their compounds was determined. The disc diffusion method carried the antibacterial activity test against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli and Salmonella typhi) bacteria. Characteristics of endophytic fungi were identified microscopically and macroscopically. Active endophytic fungi were identified by molecular analysis of the internal transcribed region (ITS) of the ribosomal DNA. Isolation of antibacterial compounds using chromatographic techniques and determination of chemical structures using spectroscopy was performed. Eight endophytic fungi were obtained from the stem bark of S. jambos, namely SJ1–SJ8. The endophytic fungus SJ6 showed the highest activity and base on phylogenetic analysis, was identified as Fusarium verticillioides. Pure compounds isolated as yellowish-white solids showed good antibacterial activity against S. aureus and S. typhi with minimum inhibitory concentration values of 64 g/mL each. Based on 1D and 2D nuclear magnetic resonance spectroscopic analysis, compound 1 was identified as 3-hydroxy-4-(hydroxy(4-hydroxyphenyl)methyl)dihydrofuran-2-on.

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Section: Discussionsupporting

confidence: 45%

Antibacterial activity of endophytic fungi isolated from the stem bark of jambu mawar (Syzygium jambos)

et al. 2022

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“…The presence of this asymptomatic fungus, internally in E. uniflora plants, should be seen as an alert, since important diseases have been frequently reported by Neofusicoccum sp. in plants of the Myrtaceae family, such as eucalyptus (Eucalyptus grandis W. Hill) and Malabar plum (Syzygium cumini (L.) Skeels) (PILLAY et al, 2013;HANIN and FITRIASARI, 2019). They can be considered asymptomatic hosts in the spread of the pathogen, mainly by means of seedlings or cuttings for regions where the disease does not occur yet.…”

Section: Resultsmentioning

confidence: 99%

Is the endophytic fungus Neofusicoccum kwambonambiense another brick in the wall of the post-harvest pathogenic fruit fungi system?

Mussi‐Dias¹,

Santos²,

Carvalho³

et al. 2021

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Entophytic fungi are characterized as microorganisms that colonize plant tissues without causing infection symptoms. These organisms are sources of genetic and biochemical diversity for numerous applications, from the production of pharmaceutical compounds to foods. However, many of these fungi can become pathogenic to plants if they find suitable substrate for their infection and development. Neofusicoccum spp. has been considered a phytopathogenic fungus for many agricultural crops, being aggressive for some of them both in their woody parts or fruits, either while still in the orchards or after being harvested. The identification and pathogenicity of the isolates are two important aspects for their characterization. Thus, the objective of this work was to characterize the pathogenicity of two endophytic isolates of the fungus Neofusicoccum sp. in different fruits in the post-harvest process. Therefore, isolates identity confirmation was obtained using Bayesian reconstruction of the internal transcribed spacer region (ITS) and translation elongation factor 1-alpha (TEF 1-α). Subsequently, the isolates were inoculated in zucchini, banana, persimmon, green beans, guava, orange, apple, melon, papaya and tomato, followed by a 10-day evaluation in order to calculate the areas under the disease progress curve. Phylogenetic reconstruction allowed identification of two isolates of the endophytic fungus of Brazilian cherry leaves as Neofusicoccum kwambonambiense, which were confirmed as pathogenic for the following fruits: Brazilian cherry, papaya, tomato, banana and apple, with variations in aggressiveness. However, no infections were found in zucchini, melon and guava by any of the isolates. This is believed to be the first occurrence of N. kwambonambiense isolated from plants of Eugenia uniflora, with pathogenicity in different post-harvest fruits.

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“…The isolation process was carried out on a PDA medium because fungal cultures grew faster on this medium and several species might not have been isolated before. The diversity of endophytic fungi isolates was not only found in S. zeylanicum but was also found in the isolation of endophytic fungi from S. cumini (Hanin and Fitriasari 2019), S. aqeueum (Habisukan et al 2021), S. aromaticum (Shofiana et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Purification was carried out by transferring the colonies to a new PDA medium with a single spore isolation method, then incubated at room temperature for 2 x 24 hours. The purified fungal colonies were re-cultured on PDA media as working culture (in Petri dishes) and stock cultures (in test tubes) (Fitriarni and Kasiamdari 2018;Hanin and Fitriasari 2019;Habisukan et al 2021).…”

Section: Isolation Of Endophytic Fungimentioning

confidence: 99%

Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite

et al. 2021

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Abstract. Syarifah, Elfita, Widjajanti H, Setiawan A, Kurniawati AR. 2021. Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite. Biodiversitas 22: 4572-4582. The decoction of the root bark of Syzygium zeylanicum has been used as traditional medicine, such as for treating pathogenic bacterial infections. Endophytic fungi that live in medicinal plant tissues have a high species diversity and biological activities correlate with their host. Therefore, this study aimed to explore the diversity of endophytic fungi from the root bark of S. zeylanicum and to determine the antibacterial activity of endophytic fungi and their secondary metabolites. In this study, we isolate and identify the endophytic fungi from the root bark of S. zeylanicum, continued by screening their antibacterial activity against two Gram-negative bacteria (Escherichia coli InaCCB5 and Salmonella thypi ATCC1048 and two Gram-positive bacteria (Staphylococcus aureus InaCCB4 and Bacillus subtilis InaCCB1204) by the Kirby-Bauer method. The fungal extract with the highest antibacterial activity proceeded with the isolation and determination of the structure of their bioactive compounds. The isolates were morphologically identified. Isolates that showed strong antibacterial activity were identified by molecular identification. Isolation of bioactive compounds was carried out by chromatographic techniques and the determination of the structure of pure chemical compounds was performed by the spectroscopic analysis. In total, there were 8 isolates of endophytic fungi were obtained from the root bark of S. zeylanicum, namely SZR1 – SZR8. SZR2 isolate has the highest antibacterial activity. Molecular identification through phylogenetic analysis showed that SZR2 isolate had high similarity with Penicillium brefeldianum. Isolation of bioactive compounds from SZR2 produced compound 1 in the form of light yellow crystals which showed strong antibacterial activity against S. typhi, E. coli, and B. subtilis with MIC values of 64 g/mL. Compound 1 was identified as p-hydroxybenzaldehyde, which was also obtained in its host. In conclusion, the endophytic fungus Penicillium brefeldianum produces similar secondary metabolites and antibacterial activity as its host plant.

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Identification of Endophytic Fungi from Fruits and Seeds of Jambolana (Syzygium cumini L.) Skeels

Cited by 12 publications

References 17 publications

Antibacterial activity of endophytic fungi isolated from the stem bark of jambu mawar (Syzygium jambos)

Antibacterial activity of endophytic fungi isolated from the stem bark of jambu mawar (Syzygium jambos)

Is the endophytic fungus Neofusicoccum kwambonambiense another brick in the wall of the post-harvest pathogenic fruit fungi system?

Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite

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