2018
DOI: 10.29244/jitkt.v9i2.19302
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ANTIBACTERIAL ACTIVITY FOR MULTI DRUG RESISTANCE (MDR) BACTERIA BYSEA CUCUMBER Stichopus vastus EXTRACT FROM KARIMUNJAWA ISLANDS – INDONESIA

Abstract: The study aims to explore the antibacterial activity of Stichopus vastus against pathogenic MDR bacteria. Analysis of samples of sea cucumbers included extraction, fractionation, and analysis of bacterial sensitivity test Gas Chromatography-Mass Spectrometry (GC-MS), the extraction process is carried out by solid-liquid extraction method. Fractionation was done with Open-Column Chromatography (OCC). Sensitivity test of bacteria was done using an agar diffusion method according to the Kirby-Bauer (Ref). The stu… Show more

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Cited by 4 publications
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“…Low (0.4 mg ml −1 ) and high (2 mg ml −1 ) concentrations of all extracts were chosen based on previous studies examining the antibacterial activities of crude sea cucumbers' ethyl acetate extracts (Mokhlesi et al ., 2012; Adibpour et al ., 2014; Pringgenies et al ., 2017; Torres, 2017). The two selected concentrations of all extracts were assessed against Bacillus subtilis ATCC 6633 and Bacillus cereus ATCC 11778 as gram-positive bacteria, and Escherichia coli ATCC 8739 and Pseudomonas aeruginosa as gram-negative bacteria.…”
Section: Methodsmentioning
confidence: 99%
“…Low (0.4 mg ml −1 ) and high (2 mg ml −1 ) concentrations of all extracts were chosen based on previous studies examining the antibacterial activities of crude sea cucumbers' ethyl acetate extracts (Mokhlesi et al ., 2012; Adibpour et al ., 2014; Pringgenies et al ., 2017; Torres, 2017). The two selected concentrations of all extracts were assessed against Bacillus subtilis ATCC 6633 and Bacillus cereus ATCC 11778 as gram-positive bacteria, and Escherichia coli ATCC 8739 and Pseudomonas aeruginosa as gram-negative bacteria.…”
Section: Methodsmentioning
confidence: 99%