Antibacterial Activity of a Human Monoclonal Antibody to Haemophilus Influenzae Type B Capsular Polysaccharide

Hunter, KennethW.; Hemming, ValG.; Fischer, GeraldW.; Wilson, SamuelR.; Hartzman, R.J.; Woody, JamesN.

doi:10.1016/s0140-6736(82)92682-4

Cited by 38 publications

(3 citation statements)

References 27 publications

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“…Human monoclonal antibodies (HmAb) represent an interesting alternative for the passive immunization of this high-risk population. In comparison to human polyvalent sera, large quantities of homogeneous and well characterized protective HmAb can be pro- [19] and Gigliotti et al [20] have reported the production of HmAb against Hib capsular polysaccharide and that these HmAb provided significant protection in an infant rat model of Hib infection.…”

Section: Introductionmentioning

confidence: 99%

Heterohybridomas secreting human monoclonal antibodies against haemophilus influenzae type b

Martin

Larose²,

Hamel³

et al. 1988

Eur J Immunol

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Seven human monoclonal antibodies (HmAb) directed against outer membrane antigens of Haemophilus influenzae type b (Hib) were produced by fusing Sp2/HPT heteromyeloma cells with human tonsillar lymphocytes sensitized in vitro for 6 days. The heterohybridomas were maintained in culture for at least one year and secreted, when cultured in Dulbecco's modified Eagle's medium without fetal calf serum, between 1 and 15 micrograms/10(6) cells/ml/24 h. All of the HmAb were IgGs except HiH-12 which is an IgM. Antibodies directed against the lipopolysaccharide and proteins of apparent molecular masses of 43, 37 and 27 kDa were identified by immunoblotting of sodium dodecyl sulfate-polyacrylamide gel electrophoresis patterns of outer membrane. Binding radioimmunoassay with live bacteria showed that five out of seven HmAb adsorbed to cell surface-exposed antigenic determinants. HmAb HiH-6, HiH-7 and HiH-10 reacted with a surface-accessible determinant on the 43-kDa outer membrane protein. In a dot enzyme immunoassay, these HmAb recognized 103 out of 111 Hib strains isolated worldwide. The strains were selected to represent the most common genotypic variations among Hib. None of these HmAb reacted with other bacterial species tested. These HmAb may serve to study the bacterial surface antigens implicated in the human humoral response and protection to Hib infections.

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Section: Introductionmentioning

confidence: 99%

Heterohybridomas secreting human monoclonal antibodies against haemophilus influenzae type b

Martin

Larose²,

Hamel³

et al. 1988

Eur J Immunol

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“…One hybridoma was constructed with hu man myeloma cells and spleen lymphocytes [28] . The antibody from one clone reacted with polyribosylribitol phosphate capsular polysaccharide from H. influenzae type b.…”

Section: Haemophilus Influenzaementioning

confidence: 99%

Antibacterial Monoclonal Antibodies and the Dawn of a New Era in the Control of Infection

Macario¹,

Macario²

1984

Pathol Immunopathol Res

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“…typhimurium; acid polysaccharides of encapsulated staphylococci; a glycolipoprotein from the surface slime of Ps. a e r u g i n o s a ; the capsular polysaccharide of H. influenzae type b; and capsular material of B a ct ero id esfra g ilis (Smith 1976, 19775 Denson & Mandell 1980Bartell & Krikszens 1980;Bortolussi & Ferrieri 1980;Wilton 1981;Quie et al 1981;Hunter et al 1982;Easmon 1983;Penn 1983;Stendahl 1983). As yet the capsular materials of the following organisms have only been associated with resistance to attachment and ingestion in vitro, although more investigation would show them strongly implicated and probably relevant to infection in vivo: meningococci, type B streptococci, Klebsiella pneumoniae, Pasteurella multocida and Campylobacter fetus (Densen & Mandell 1980;Robbins et al 1980;Wilton 1981;Quie et al 1981;Easmon 1983;Penn 1983).…”

Section: {B) Interference With the Phagocytic Defencesmentioning

confidence: 99%

The elusive determinants of bacterial interference with non-specific host defences

Smith

Cleat²,

Veale³

et al. 1983

Phil. Trans. R. Soc. Lond. B

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The identification of the determinants of bacterial interference with non-specific host defences during the early stages of infection is approached rather than attained. Recognizing a relevant biological property (e.g. resistance to phagocytosis) by an in vitro test and associating bacterial surface components with it are relatively easy. Proving causation, however, is usually not completed because the biological test is complex and the surface component(s) act only in situ . Nevertheless, evidence in addition to mere association can be sought to show that a putative determinant is strongly implicated in biological activity. Even then, proving that the biological activity concerned is relevant to infection in vivo , and that the putative determinant is produced there, is often not accomplished. Again, however, distinction can be made between those cases probably relevant in vivo and those only possibly so. Finally, bacteria grown in vitro can be deficient in some of the determinants of pathogenicity expressed during infection, and this situation requires the study of organisms grown in vivo . These points are discussed and then illustrated in a brief survey of the activities of many pathogenic bacteria and a description of recent work on the resistance of gonococci to killing by human serum and phagocytes.

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Antibacterial Activity of a Human Monoclonal Antibody to Haemophilus Influenzae Type B Capsular Polysaccharide

Cited by 38 publications

References 27 publications

Heterohybridomas secreting human monoclonal antibodies against haemophilus influenzae type b

Heterohybridomas secreting human monoclonal antibodies against haemophilus influenzae type b

Antibacterial Monoclonal Antibodies and the Dawn of a New Era in the Control of Infection

The elusive determinants of bacterial interference with non-specific host defences

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