Antibacterial activity of antimicrobial peptide-conjugated nanofibrous membranes

Onak, Günnur; Ercan, Utku Kürşat; Karaman, Ozan

doi:10.1088/1748-605x/abb722

Cited by 9 publications

(5 citation statements)

References 68 publications

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“…Recently, Günnur et al 76 introduced carboxyl groups into the surface of electrospun poly (lactic‐co‐glycolic acid) (PLGA) nanofibers by cold atmospheric plasma (CAP) and immobilized antibacterial peptide RW/KRF on the membrane surface by EDC/NHS. When the membrane was contacted with P. aeruginosa and MRSA for 6 h, the bacteria died significantly (3 log P. aeruginosa and 2.5 log MRSA reduction, respectively).…”

Section: Fight Arb With Alternative Weaponsmentioning

confidence: 99%

Nanofibrous dressing: Potential alternative for fighting against antibiotic‐resistance wound infections

Yingjie

Zhang

et al. 2022

J of Applied Polymer Sci

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The progression of antibiotic‐resistant bacteria (ARB) as well as impaired immunity due to chronic diseases and complications, aging, and so forth pose opportunities in parallel with challenges to the wound dressing products. In a bid to address the remedies, electrospun nanofibrous membranes (ENMs) with high permeability and good biocompatibility have made an entry as wound dressings. With high specific surface area, such ENMs can pave the way for the loading and release of antimicrobial agents and further promote wound healing by mimicking the extracellular matrix structure. In this review, we have compiled a series of novel antimicrobial agents that can be substituted for antibiotics against ARB and outlined their antimicrobial mechanisms, providing an overview of the latest research to illustrate how ENMs can achieve the loading and efficient release of antimicrobial agents against ARB. Last but not least, on the basis of the demands for timely and accurate wound diagnosis, ENMs capable of colorimetric detection are featured as test‐strips for the diagnosis of wound pathogenic bacteria, in the hope of inspiring researchers to design smart wound dressings capable of diagnosis and treatment integration.

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Section: Fight Arb With Alternative Weaponsmentioning

confidence: 99%

Nanofibrous dressing: Potential alternative for fighting against antibiotic‐resistance wound infections

Yingjie

Zhang

et al. 2022

J of Applied Polymer Sci

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“…RW (RWRWRWRW; Arg-Trp-Arg-Trp-Arg-Trp-Arg-Trp-NH 2 ) and KRF (KRFRIRVRV; Lys-Arg-Phe-Ile-Arg-Val-Arg-Val-NH 2 ) were synthesized by the application of 9-fluorenylmethoxycarbonyl (Fmoc) chemistry as previously indicated by the help of the automated peptide synthesis device (AAPPTEC Focus Xi, Louisville, KY, USA) with use of 4-Methylbenzhydrylamine (MBHA) resin (substitution = 0.67 mol/g). [51][52][53] For each peptide sequence, 100 mg resins were swollen inside Dimethylformamide (DMF) for 30 min after resins were washed three times by DMF. After that, 20% piperidine includingDMF solution was transferred to the resins to remove Fmoc-protecting group, then the resins were waited and stirred inside 20% piperidine-DMF solution for 30 min and the Kaiser test was applied for checking the elimination of the Fmoc-protecting group.…”

Section: Peptide Synthesis and Characterizationmentioning

confidence: 99%

“…50 In addition, Onak et al demonstrated that RWRWRWRW-NH 2 has an antimicrobial effect on MRSA and P. aeruginosa over 20 μ m , while KRFRIRVRV-NH 2 has an antimicrobial effect on both strains above 100 µM. 51…”

Section: Introductionmentioning

confidence: 99%

“…50 In addition, Onak et al demonstrated that RWRWRWRW-NH 2 has an antimicrobial effect on MRSA and P. aeruginosa over 20 μM, while KRFRIRVRV-NH 2 has an antimicrobial effect on both strains above 100 µM. 51 AMPs conjugated sutures might be a favorable opportunity to obtain antimicrobial efficiency for the control and prevention of SSIs by both promoting wound healing and avoiding drug resistance. To the best of our knowledge, there is no performed study regarding AMP conjugation on surgical suture surfaces.…”

Section: Introductionmentioning

confidence: 99%

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Effect of antimicrobial peptide conjugated surgical sutures on multiple drug-resistant microorganisms

et al. 2022

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Surgical site infections are commonly encountered as a risk factor in clinics that increase the morbidity of a patient after a surgical operation. Surgical sutures are one of the leading factor for the formation of surgical site infections that induce bacterial colonization by their broad surface area. Current strategies to overcome with surgical site infections consist utilization of antibiotic agent coatings such as triclosan. However, the significant increase in antibiotic resistance majorly decreases their efficiency against recalcitrant pathogens such as; Pseudomonas aeruginosa and Staphylococcus aureus. Therefore, the development of a multi drug-resistant antimicrobial suture without any cytotoxic effect to combat surgical site infections is vital. Antimicrobial peptides are the first defense line which has a broad range of spectrum against Gram-positive, and Gram-negative bacteria and even viruses. In addition, antimicrobial peptides have a rapid killing mechanism which is enhanced by membrane disruption and inhibition of functional proteins in pathogens without the development of antimicrobial resistance. In the scope of the current study, the antimicrobial effect of antimicrobial peptide conjugated poly (glycolic acid-co-caprolactone) (PGCL) sutures were investigated against P. aeruginosa and methicillin-resistant S. aureus (MRSA) strains by using antimicrobial peptide sequences of KRFRIRVRV-NH2, RWRWRWRW-NH2 and their dual combination (1:1). In addition, in vitro wound scratch assays were performed to evaluate the effect of antimicrobial peptide conjugated sutures on keratinocyte cell lines. Our results indicated that antimicrobial peptide modified sutures could be a potential novel medical device to overcome surgical site infections by the superior acceleration of wound healing.

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“…Although various peptides have been successfully used to modify cellulose, current modification methods still meet various drawbacks. For example, traditional cellulose modification methods such as silane coupling agent modification and peroxide modification always involve toxic and harmful reagents, strict reaction conditions, and cumbersome and complicated modification process [30][31][32] .…”

Section: Introductionmentioning

confidence: 99%

A green, efficient and economical polypeptide—modified bamboo fiber and its application in glycopeptide antibiotics adsorption

et al. 2023

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Inspired by wet-cool/dry-hot cycle drived prebiotic reactions, a green and efficient dry-wet cycle modification method was proposed for the preparation of polypeptide-modified bamboo fiber (P-MBF). Functionalization of P-MBF was characterized by Fourier transform infrared spectroscopy, X-ray diffraction, element analysis and scanning electron microscopy. Compared to raw bamboo fiber materials, P-MBF showed better dispersibility in aqueous solutions, and thus exhibited better adsorption performance for glycopeptide antibiotics in sewage treatment. With vancomycin as the target antibiotic, adsorption performance of P-MBF was studied in aqueous solution. Adsorption kinetics and isotherms were well correlated with pseudo-second-order model and Langmuir model, respectively. Investigation of thermodynamic parameters implied that the adsorption was a spontaneous exothermic process. The adsorption efficiencies in standard solution and drinking water were 97.83% and 91.32% , respectively. This study provides a green, efficient and economical modified bamboo fiber material for the removal of glycopeptide antibiotics from sewage samples.

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Antibacterial activity of antimicrobial peptide-conjugated nanofibrous membranes

Cited by 9 publications

References 68 publications

Nanofibrous dressing: Potential alternative for fighting against antibiotic‐resistance wound infections

Nanofibrous dressing: Potential alternative for fighting against antibiotic‐resistance wound infections

Effect of antimicrobial peptide conjugated surgical sutures on multiple drug-resistant microorganisms

A green, efficient and economical polypeptide—modified bamboo fiber and its application in glycopeptide antibiotics adsorption

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