Antibacterial activity of bryophyte species against Paenibacillus larvae isolates

Abstract

This study was performed to determine the antibacterial activity of methanol extracts of 23 bryophyte species against Paenibacillus larvae isolates that cause American foulbrood diseases in honeybee larvae. The honey and larva samples were collected from nine different locations of Rize in Turkey. A total of 22 gram-positive spore-forming bacteria were isolated from the larva and honey samples. According to the results of morphological, biochemical, and molecular (16S rRNA gene sequencing) tests, 10 isolates of the 22 gram-positive spore-forming bacteria were identified as P. larvae. A total of 10 bryophyte species (Polytrichum formasum, Polytrichum commune, Calliergonella cuspitada, Calliergonella lindbergi, Metzgeria conjugata, Isothecium alopecuroides, Syntrichia calcicola, Syntrichia intermedia, Tortella densa, and Grimmia alpestris) among 23 bryophytes showed good antimicrobial activity against P. larvae isolates according the results of agar-well diffusion method and minimal inhibition concentration experiments.

Antimicrobial and antioxidant activities and volatile constituents of Eurhynchium angustirete (Broth.) T. J. Kop. and Isothecium alopecuroides (Lam. ex Dubois) Isov. from Turkey

“…In another study, methanol extract of I. alopecuroides, was shown to have an effective antimicrobial effect on P. larve isolates (Sevim et al, 2017). Previous studies confirm the high antimicrobial activity values that this species showed in our study.…”

Chemical composition and antimicrobial and antioxidant activities of essential oils of Polytrichum commune (Hedw.) and Antitrichia curtipendula (Hedw.) Brid. grown in Turkey

“…In 2013, Cheng showed that ethyl acetate extract of this species stimulates apoptosis and increases oxidative stress in L1210 cells (Cheng et al, 2013). In another study with methanol extract of P. commune, the species have been shown to have an effective antimicrobial effect on P. larve isolates (Sevim et al, 2017). These studies support the high antimicrobial activity that this species exhibited in our study.…”

“…Polytrichum commune as moss class consumed as boiled tea was chosen as the study material for this specific study because it is used in the treatment of many diseases such as wound healing, antipyretic, antidotal activity, dissolving kidney and gallbladder stones, antipyretic and antipyretic, and colds (Chandra et al, 2017;Hallingback et al, 2000;Greeshma & Murugan 2018). There are a few reports on the antibacterial, cytotoxicity, and antimicrobial activities of solvent extract of Polytrichum commune grown in different parts of the world including Turkey (Klavina et al, 2015;Nikolajeva et al, 2012;Sevim et al, 2017).…”

Differences in use of bryophyte species in tit nests between two contrasting habitats: an urban park and a forest

“…Wesołowski and Wierzcholska (2018) showed that moss species selectivity related to water-absorbing function was not the case as the water-uptake ratios did not differ between used and not-used moss species. On the other hand, many bryophyte species are known for their medical properties (antimicrobial, antifungal, antiseptic;Asakawa 2007;Bukvički et al 2012;Sevim et al 2017), and this may be the next reason why those birds are quite choosy when collecting bryophyte, but unfortunately there are practically no studies that examine those properties in tit nests and more studies on this subject would be welcome. In a study where tit nests were vacuum-packed and tested for insulation properties, Deeming and Biddle (2015) suggested tremble-thrusting in tits as a way to add air gaps in the moss-made nest wall.…”