Antibacterial Activity of Cassava Manihot Esculenta Leaves Extract Against Escherichia coli

Abstract

The purpose of this research is to determine the antibacterial activity of Cassava (Manihot esculenta) leaf extract against Escherichia coli. The experiment involved the determination of plants, extraction using the maceration method, and antibacterial testing of the fresh and dried cassava (Manihot esculenta) leave extracted with concentrations of 5%, 10%, 20%, and 30% ethanol against the bacterial growth of (Escherichia coli) and culture media using Merck Nutrient Agar. A caliper was used to measure the size of (Escherichia coli) growth in millimeters. The results showed that extracts of fresh and dried cassava (Manihot esculenta) leaves extract with ethanol concentrations of 5%, 10%, 20%, and 30% have antibacterial activity against Escherichia coli. This study examined the efficacy of fresh and dried cassava (Manihot esculenta) leaves extract against (Escherichia coli) in the 18th and 24th hours. The mean of fresh cassava (Manihot esculenta) leaves extract is 4.4, which differs considerably from the mean of dried Cassava (Manihot esculenta) leaves extract, which is 3.9. The fresh cassava (Manihot esculenta) leaves extract was the most potent therapy against (Escherichia coli) in the 18th hour, according to the findings of this investigation. Furthermore, at the 24th hour, the fresh cassava (Manihot esculenta) leaves extract was the most potent therapy against (Escherichia coli). Fresh cassava (Manihot esculenta) leaves extract has a mean of 4.4, which differs significantly from dried (Manihot esculenta) leaves extract, which has a mean of 3.9. According to the findings of this study's testing, the 20 percent concentration of fresh cassava (Manihot esculenta) leaves extract with an average of 5.75 was the most active treatment against (Escherichia coli). It has been proposed that cassava (Manihot esculenta) leaves could be turned into standardized antibacterial herbal. Future research should investigate the effects of cassava (Manihot esculenta) leaves used as an antibacterial agent on human health.

The Antibacterial and Antioxidant Activities of Endophytic Bacteria from Cassava Leaves (Manihot esculenta Crantz)

“…Cassava leaf extract has antibacterial activity against Staphylococcus epidermidis, Propionibacterium acnes, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. 2,3,4 In addition, cassava leaves are also known to have a role as antioxidants. According to Linn et al Cassava leaves have significant antioxidant activity when tested individually in vitro.…”

“…6 The results of phytochemical analysis prove that cassava leaves contain alkaloids, saponins, flavonoids and tannins that act as antibacterials as well as phenolics and flavonoids that act as antioxidants. 4,5 © 2023 the authors. This work is licensed under the Creative Commons Attribution 4.0 International License and taken to the Microbiology Laboratory of the Faculty of Mathematics and Natural Sciences, Syiah Kuala University to be washed and sterilized.…”