The purpose of this research is to determine the antibacterial activity of Cassava (Manihot esculenta) leaf extract against Escherichia coli. The experiment involved the determination of plants, extraction using the maceration method, and antibacterial testing of the fresh and dried cassava (Manihot esculenta) leave extracted with concentrations of 5%, 10%, 20%, and 30% ethanol against the bacterial growth of (Escherichia coli) and culture media using Merck Nutrient Agar. A caliper was used to measure the size of (Escherichia coli) growth in millimeters. The results showed that extracts of fresh and dried cassava (Manihot esculenta) leaves extract with ethanol concentrations of 5%, 10%, 20%, and 30% have antibacterial activity against Escherichia coli. This study examined the efficacy of fresh and dried cassava (Manihot esculenta) leaves extract against (Escherichia coli) in the 18th and 24th hours. The mean of fresh cassava (Manihot esculenta) leaves extract is 4.4, which differs considerably from the mean of dried Cassava (Manihot esculenta) leaves extract, which is 3.9. The fresh cassava (Manihot esculenta) leaves extract was the most potent therapy against (Escherichia coli) in the 18th hour, according to the findings of this investigation. Furthermore, at the 24th hour, the fresh cassava (Manihot esculenta) leaves extract was the most potent therapy against (Escherichia coli). Fresh cassava (Manihot esculenta) leaves extract has a mean of 4.4, which differs significantly from dried (Manihot esculenta) leaves extract, which has a mean of 3.9. According to the findings of this study's testing, the 20 percent concentration of fresh cassava (Manihot esculenta) leaves extract with an average of 5.75 was the most active treatment against (Escherichia coli). It has been proposed that cassava (Manihot esculenta) leaves could be turned into standardized antibacterial herbal. Future research should investigate the effects of cassava (Manihot esculenta) leaves used as an antibacterial agent on human health.

Climate change compounds matters because agriculture is dependent on land. Soilless system production is attractive since it allows for the use of unproductive land for agriculture while reducing water use. The light intensities in one-, two-, and three-layer treatments affect Brassica rapa growth and development, according to the discussion and conclusions. The three-layer net group outgrew the one-layer control group in terms of growth and development. Brassica rapa's development and maturity were influenced by light intensities. Plant growth and yield improve as the number of Brassica rapa layers increases. Varying effects on plant output, fresh weight, water consumption, and leaf tissue in the intensities of light. The study discovered that light had an impact potentially in Brassica rapa in hydroponic systems. The temperature of the environment can stymie development, manufacturing, and diffusion. The findings indicate that the cultivar is more resistant to light stress.