Antibacterial Activity of Cold Atmospheric Pressure Argon Plasma against 78 Genetically Different (&lt;b&gt;&lt;i&gt;mecA&lt;/i&gt;&lt;/b&gt;, &lt;b&gt;&lt;i&gt;luk-P&lt;/i&gt;&lt;/b&gt;, &lt;b&gt;&lt;i&gt;agr&lt;/i&gt;&lt;/b&gt; or Capsular Polysaccharide Type) &lt;b&gt;&lt;i&gt;Staphylococcus aureus &lt;/i&gt;&lt;/b&gt;Strains

Matthes, Rutger; Lührman, Anne; Holtfreter, Silva; Kolata, Julia; Radke, Dörte; Hübner, Nils–Olaf; Assadian, Ojan; Krämer, Axel

doi:10.1159/000443210

Cited by 25 publications

(24 citation statements)

References 54 publications

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“…, Matthes et al . ). Due to the wound size, we only reached a mean treatment time of 30 seconds per cm 2 if we limited total treatment to a maximum of 15 minutes, which might not have been enough.…”

Section: Discussionmentioning

confidence: 97%

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Comparison of polyhexanide, cold atmospheric plasma and saline in the treatment of canine bite wounds

Nolff

Winter

Reese

et al. 2018

J of Small Animal Practice

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Objectives To compare the efficacy of polyhexanide, cold argon plasma and saline in reducing bacterial bio‐burden in dog bite wounds. Materials and Methods Prospective blinded randomised clinical trial. Dogs were randomly assigned to one of the treatment groups by lottery and bacterial cultures obtained before and after treatment were compared. Bite wounds were surgically debrided and treated with polyhexanide, cold argon plasma or saline lavage. All wounds were cultured three times: directly after debridement, directly after prelavage with 2 mL/cm2 (saline in the saline and cold argon plasma group, or polyhexanide) and following the definitive lavage. Data were analysed using a generalised linear model for ordinal data. Results A total of 85 dogs were enrolled in this study (polyhexanide n=29, cold argon plasma n=28, saline n=28). Positive bacterial culture results after debridement were obtained in 53/85 (62.3%) wounds. Polyhexanide and saline lavage significantly reduced the bio‐burden, while cold argon plasma treatment did not. This effect was evident after prelavage when polyhexanide performed significantly better than saline and cold argon plasma as well as after final treatment. No significant differences were detected after prelavage or main treatment between saline and cold argon plasma. Clinical Significance Polyhexanide lavage achieved the best immediate and ultimate decontamination of bite wounds.

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Section: Discussionmentioning

confidence: 97%

“…, Matthes et al . ). In addition to the in vitro studies, initial in vivo trials also showed a profound wound decontamination effect (Hammann et al .…”

Section: Introductionmentioning

confidence: 97%

Comparison of polyhexanide, cold atmospheric plasma and saline in the treatment of canine bite wounds

Nolff

Winter

Reese

et al. 2018

J of Small Animal Practice

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“…The microbicidal effect of CAP was observed in vitro [256,257,258,259,260,261] on skin and chronic wounds, and exceeds the effectiveness of CHD, PVP-I, and PHMB. In a 3D epidermis model, CAP displayed dose- and time-dependent compatibility [256]; P. aeruginosa was inactivated without destroying the structure of the epidermis.…”

Section: Future Perspectivesmentioning

confidence: 99%

Consensus on Wound Antisepsis: Update 2018

Krämer

Dissemond²,

Kim³

et al. 2017

Skin Pharmacol Physiol

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256

215

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Wound antisepsis has undergone a renaissance due to the introduction of highly effective wound-compatible antimicrobial agents and the spread of multidrug-resistant organisms (MDROs). However, a strict indication must be set for the application of these agents. An infected or critically colonized wound must be treated antiseptically. In addition, systemic antibiotic therapy is required in case the infection spreads. If applied preventively, the Wounds-at-Risk Score allows an assessment of the risk for infection and thus appropriateness of the indication. The content of this updated consensus recommendation still largely consists of discussing properties of octenidine dihydrochloride (OCT), polihexanide, and iodophores. The evaluations of hypochlorite, taurolidine, and silver ions have been updated. For critically colonized and infected chronic wounds as well as for burns, polihexanide is classified as the active agent of choice. The combination 0.1% OCT/phenoxyethanol (PE) solution is suitable for acute, contaminated, and traumatic wounds, including MRSA-colonized wounds due to its deep action. For chronic wounds, preparations with 0.05% OCT are preferable. For bite, stab/puncture, and gunshot wounds, polyvinylpyrrolidone (PVP)-iodine is the first choice, while polihexanide and hypochlorite are superior to PVP-iodine for the treatment of contaminated acute and chronic wounds. For the decolonization of wounds colonized or infected with MDROs, the combination of OCT/PE is preferred. For peritoneal rinsing or rinsing of other cavities with a lack of drainage potential as well as the risk of central nervous system exposure, hypochlorite is the superior active agent. Silver-sulfadiazine is classified as dispensable, while dyes, organic mercury compounds, and hydrogen peroxide alone are classified as obsolete. As promising prospects, acetic acid, the combination of negative pressure wound therapy with the instillation of antiseptics (NPWTi), and cold atmospheric plasma are also subjects of this assessment.

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“…Herbert, Rossaint, Khanna, and Kreyenschmidt () suggested that argon can induce changes in protein conformation, interfere with oxygen receptor sites of enzymes, and can displace oxygen more effectively than nitrogen. DBD‐HVCAP generated with argon gas has promising bacterial inactivation efficacy (Matthes et al., ; Olatunde et al., ). Nevertheless oxygen has been employed as the working gas for generation of ROS, most especially ozone, which has been known as an antimicrobial agent (Olatunde & Benjakul, ).…”

Section: Introductionmentioning

confidence: 99%

Dielectric Barrier Discharge High Voltage Cold Atmospheric Plasma: An Innovative Nonthermal Technology for Extending the Shelf‐Life of Asian Sea Bass Slices

Olatunde

Benjakul

Vongkamjan

2019

Journal of Food Science

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Impact of dielectric barrier discharge high‐voltage cold atmospheric plasma (DBD‐HVCAP) generated with the mixture of oxygen and argon (10:90) for various treatment times (2.5 to 10 min) on the qualities of Asian sea bass slices during 4 °C storage was investigated. Microbial load of slices treated with DBD‐HVCAP were lower than the control. The efficacy of bacteria reduction by DBD‐HVCAP was dependent on the treatment times (P < 0.05). Total viable bacteria count (TVBC) was more than 6.0 Log CFU/g at day 6 for the control kept in air. Slices treated with DBD‐HVCAP for all treatment times used had TVBC lower than the limit at day 12. Total volatile nitrogen base content (TVNB) as well as trimethylamine (TMA) content in slices treated with DBD‐HVCAP were lower than that of the control throughout the storage. TVNB as well as TMA contents were lower in HVCAP treated slices in a treatment time‐dependent manner. Nevertheless, lipid oxidation in samples treated with DBD‐HVCAP was higher than that of the control. Polyunsaturated fatty acids were decreased in slices treated with DBD‐HVCAP for more than 5 min after 12 days of storage. Therefore DBD‐HVCAP treatment for 5 min was demonstrated to be potential means for increasing the shelf‐life of Asian sea bass slices with minimal negative effect on chemical and sensory properties, in which they could be stored longer than 12 days at 4 °C. Practical Application Microbial inactivation capacity of dielectric barrier discharge high‐voltage cold atmospheric plasma (DBD‐HVCAP) has been documented with limited information on its application in extending the shelf‐life of foods. DBD‐HVCAP was demonstrated as an innovative technology for extending the shelf‐life of Asian sea bass slices, which could be implemented in seafood industries for assuring safety and extending shelf‐life of products. The shelf‐life of the slices treated with DBD‐HVCAP was extended to 12 days of storage at 4 °C as compared to the 6 days of the untreated counterpart.

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Antibacterial Activity of Cold Atmospheric Pressure Argon Plasma against 78 Genetically Different (<b><i>mecA</i></b>, <b><i>luk-P</i></b>, <b><i>agr</i></b> or Capsular Polysaccharide Type) <b><i>Staphylococcus aureus </i></b>Strains

Cited by 25 publications

References 54 publications

Comparison of polyhexanide, cold atmospheric plasma and saline in the treatment of canine bite wounds

Comparison of polyhexanide, cold atmospheric plasma and saline in the treatment of canine bite wounds

Consensus on Wound Antisepsis: Update 2018

Dielectric Barrier Discharge High Voltage Cold Atmospheric Plasma: An Innovative Nonthermal Technology for Extending the Shelf‐Life of Asian Sea Bass Slices

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