Antibacterial Activity of Endophytic Fungus Isolates of Mangrove Fruit (Sonneratia alba) Against Staphylococcus aureus and Esherichia coli

Abstract

Endophytic fungi live and associate in plant tissues and have a mutualistic relationship. Endophytic fungi produce various compounds such as steroids, terpenoids, phenolics, alkaloids which are the same as secondary metabolites from their host plants. The objective of this study is to isolate and identify endophytic fungi from mangrove fruit (Sonneratia alba) and to determine the antibacterial activity of endophytic fungi isolates against the growth of Staphylococcus aureus and Escherichia coli. The type of research is pre-experimental design, one shoot case study. The methods used are isolation, identification and agar diffusion. The pieces of mangrove fruit were disinfected and then cultured on SDA media to grow endophytic fungi isolates. The isolates were cultured repeatedly until pure isolates were obtained. The test of isolate activity against antibacterial was determined by the agar diffusion method with the test material of 2 isolates of endophytic fungi on Nutrient Agar (NA) media. The results showed that the mangrove fruit (Sonneratia alba) produced two isolates of endophytic fungi that could inhibit the growth of Staphylococcus aureus and Escherichia coli. It was concluded that the mangrove fruit culture produced 2 isolates, which are isolate 1 Aspergillus niger and isolate 2 Aspergillus flavus. Isolate 1 and isolate 2 had the potential as antibacterial against the growth of Staphylococcus aureus and Escherichia coli. Isolate 2 was more effective than isolate 1 in inhibiting the growth of Staphylococcus aureus. It is recommended to test the pharmacological and microbiological activity of the findings of isolates 1 and 2 in vivo.

Isolasi, Identifikasi dan Aktivitas Antibakteri Fungi Endofit dari Daun Jarak Pagar (Jatropha curcas L.) terhadap Escherichia coli dan Salmonella typhi

“…Hasil inkubasi ditemukan beberapa jenis endofit murni berdasarkan pengamatan bentuk dan warna koloni pada media PDA. Setiap koloni yang berbeda bentuk atau warna dikultur kembali berulang-ulang hingga diperoleh isolat koloni murni fungi endofit (Pakadang et al, 2021).…”

Isolasi, Identifikasi, dan Uji Aktivitas Antibakteri Fungi Endofit Daun Alpukat (Persea Americana Mill) Terhadap Pseudomonas aeruginosa dan Staphylococcus aureus

“…Sebelum disterilkan dalam oven pada suhu 160 0 C selama dua jam untuk peralatan gelas dan tahan panas, sebelumnya peralatan dicuci dengan menggunakan detergen dan kemudian dibilas dengan air mengalir. Sedangkan alat yang tidak tahan panas tinggi dalam autoklaf selama 15 menit pada tekanan 1 atmosfer dan suhu 121 0 C, dan untuk alat logam didesinfeksi dengan lampu pijar menggunakan lampu spiritus (Pakadang et al, 2021).…”

“…Kemudian dilakukan pengukuran zona hambatan dengan melihat adanya zona bening yang terbentuk. Pengujian dilakukan dengan 4 kali ulangan (Pakadang et al, 2021).…”

Pre-Treatments of Sonneratia Alba Fruit as The Potential Feed for Ruminant Using Aspergillus Niger at Different Fermentation Times: Tannin Concentration, Enzyme Activity and Total Colonies

“…Sonneratia alba fruit is not poisonous, so it can be eaten immediately. The benefits of S. alba fruit are the sour fruit can be used to make vinegar or syrup, produces pectin, has antioxidant activity, anti-Escherichia coli, effective for increasing appetite, and breath root as a substitute for cork and floaters (Wintah et al 2021;Pakadang et al 2021;Riyadi et al 2022). Apart from being used as a food ingredient, S. alba fruit has the potential to be used as a feed ingredient for ruminants because it contains flavonoid compounds that can enhance rumen microbial activity.…”