Inulin degrading bacteria is a potential source of inulin degrading enzymes, an enzyme which convert inulin into fructose and fructooligo-saccharides (FOS) prebiotic. The purpose of the study was to find inulin degrading bacteria. The methods that used to find inulin degrading bacteria were indirect and direct isolation method using inulin as the sole carbon source. Bacteria was characterized colony morphology. Inulin degrading bacteria were screened from two rizosphere dahlia tuber in West Sumatera (Solok and Padang Panjang). In the research has been found five inulin degrading bacteria isolates. Isolate bacteria RZ-01, RZ-02 from rizosphere dahlia tuber in Padang Panjang, isolate bacteria A1-KG, A2-KG and UKG from rizosphere dahlia tuber in Solok. All isolates grow at room temperature and 40ºC. All isolates were classified as mesophilic bacteria. Shape and colour of RZ-01, A2-KG and UKG colonies were circular and white respectively. RZ-02, A1-KG colonies were circular and yellowish.

No abstract

Endophytic fungi live and associate in plant tissues and have a mutualistic relationship. Endophytic fungi produce various compounds such as steroids, terpenoids, phenolics, alkaloids which are the same as secondary metabolites from their host plants. The objective of this study is to isolate and identify endophytic fungi from mangrove fruit (Sonneratia alba) and to determine the antibacterial activity of endophytic fungi isolates against the growth of Staphylococcus aureus and Escherichia coli. The type of research is pre-experimental design, one shoot case study. The methods used are isolation, identification and agar diffusion. The pieces of mangrove fruit were disinfected and then cultured on SDA media to grow endophytic fungi isolates. The isolates were cultured repeatedly until pure isolates were obtained. The test of isolate activity against antibacterial was determined by the agar diffusion method with the test material of 2 isolates of endophytic fungi on Nutrient Agar (NA) media. The results showed that the mangrove fruit (Sonneratia alba) produced two isolates of endophytic fungi that could inhibit the growth of Staphylococcus aureus and Escherichia coli. It was concluded that the mangrove fruit culture produced 2 isolates, which are isolate 1 Aspergillus niger and isolate 2 Aspergillus flavus. Isolate 1 and isolate 2 had the potential as antibacterial against the growth of Staphylococcus aureus and Escherichia coli. Isolate 2 was more effective than isolate 1 in inhibiting the growth of Staphylococcus aureus. It is recommended to test the pharmacological and microbiological activity of the findings of isolates 1 and 2 in vivo.

Lumpur aktif dari instalasi pengolahan air limbah industri tekstil mengandung berbagai jenis mikroorganisme antara lain mikroorganisme yang memiliki aktivitas lipase yang tinggi. Tujuan penelitian ini adalah mengisolasi dan mengkarakterisasi bakteri penghasil enzim lipase ekstraseluler dari lumpur aktif instalasi pengolahan air limbah industri tekstil. Tahapan penelitian yang dilakukan meliputi isolasi bakteri penghasil enzim lipase ekstraseluler dari lumpur aktif melalui skrining dengan media yang mengandung rodamin dan minyak zaitun, penentuan aktivitas enzim lipase dan karakterisasi lipase yang dihasilkan terhadap variasi temperatur, pH, dan pengaruh ion Ca2+. Hasil identifikasi secara mikrobiologi menunjukkan bahwa bakteri penghasil enzim lipase ekstraseluler dari lumpur aktif adalah spesies Erwiniachrysantemi. Enzim lipase ekstraseluler tersebut memiliki aktivitas 4,75 U/mL dengan temperatur optimum 40oC dan pH optimum 9. Penambahan ion Ca2+ tidak memberikan pengaruh berarti terhadap aktivitas enzim lipase.

No abstract