Antibacterial Activity of Ethanol Extract from Stem Bark and Leaves of ­Berenuk (<i>Crescentia cujete</i> L.)

Abstract

Crescentia cujete L. (C. cujete) has been known as a medicine for various diseases that caused by microorganisms. This research was aimed to identify the phytochemical compounds and to determine the antibacterial activity of ethanolic extracts from stem bark and leaves of C. cujete. The phytochemical compounds in both extracts were identified by Harborne method, while antibacterial activity assay was performed by disc diffusion method with the concentration of bacteria 106 cfu/mL. Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) were used in antibacterial assay. Minimum Inhibitory Concentration (MIC) was obtained by using contact method. The phytochemical compounds analysis showed that ethanol extracts of C. cujete stem bark and leaves contained alkaloids, flavonoids, saponins, tannins, and steroids. The results of antibacterial activity test showed that both extracts have antibacterial activity with the highest inhibition showed by extracts with concentration of 100 %(w/v). The decreased percentage in the number of bacterial colonies on the extracts was less than 90 % so that the MIC value of both extracts against S. aureus could not be determined.

Cytotoxic Activity Test Of Ethanolic Extract Of Berenuk Fruit (Crescentia Cujete L.) On Artemia Salina Leach Shrimp Larva Using Brine Shrimp Lethality Test (BSLT) Method

“…Salah satu tanaman yang berkhasiat obat adalah berenuk (Crescentia cujete L.). Berenuk yang dikenal juga dengan sebutan Mojo pahit dan bagian tanamannya yaitu daging buah, akar, daun, maupun kulit batangnya sudah cukup lama digunakan sebagai pengobatan tradisional (3). Buah berenuk berwarna hijau dengan bentuk bulat-bundar dengan diameter mencapai 25 cm, daging buah berenuk mengandung bulir warna putih, bentuk biji yang kecil dan pipih berwarna putih (4).…”

AKTIVITAS BIOINSEKTISIDA EKSTRAK DAUN MAJA (Crencentia cujete Linn.) TERHADAP Rhipicephalus sanguineus DARI ANJING LOKAL

“…Khasiat obat dari maja sama seperti tumbuhan berkhasiat obat lainnya berasal dari aktivitas metabolit sekunder seperti flavonoid, saponin, golongan alkaloid, terpenoid dan tannin. Oleh masyarakat Indonesia, bagian tanaman maja seperti daging buah, daun dan kulit batang umumnya digunakan untuk mengobati berbagai gangguan kesehatan baik pada sistem gastrointestinal, sistem respirasi, maupun neoplasma atau tumor (Hasanah et al, 2017). Selain itu, maja (Crescentia cujete Linn.)…”