Antibacterial Activity of Extract, Fractions and Four Compounds Extracted from Piper solmsianum C. DC. var. solmsianum (Piperaceae)

Campos, Marina Pereira De; Filho, Valdir Cechinel; Silva, Rosi Z.; Yunes, Rosendo A.; Monachè, Franco Delle; Cruz, Alexandre Bella

doi:10.1515/znc-2007-3-404

Cited by 15 publications

(15 citation statements)

References 29 publications

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“…Phytochemical studies revealed the presence of monoterpenes, sesquiterpenes, arylpropanoids, phenylpropanoids, lignans and neolignans, most of which showing biological activity against bacteria, fungus and the trypomastigote form of Trypanossoma cruzi (Moreira et al 1995;Martins et al 2000;Campos et al 2005;2007).…”

Section: Introductionmentioning

confidence: 99%

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

et al. 2009

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RESUMO -(Morfogênese in vitro e estabelecimento de culturas de suspensão celular em Piper solmsianum C. DC. (Piperaceae)). Piper solmsianum é uma espécie herbácea do sudeste brasileiro onde vários compostos biologicamente ativos já foram identificados. O objetivo deste trabalho foi estabelecer suspensões celulares nesta espécie. Para tanto, foram utilizados explantes de pecíolos e folhas, retirados de plântulas cultivadas in vitro, os quais foram submetidos a diferentes combinações de reguladores de crescimento (AIA, ANA, 2,4-D e BAP). Foi obtida a neo-formação de raízes e brotos, estes últimos através do processo de organogênese indireta evidenciada por estudos histológicos. Para a indução e crescimento dos calos, foram avaliados, além das diferentes combinações de reguladores de crescimento, a suplementação ao meio de cultura de carvão ativado (1,5 mg.l -1 ) e o regime de luz. Culturas mantidas na luz, em meio de cultura suplementado com 0,2 mg.l -1 2,4-D e 2 mg.l -1 BAP e sem carvão ativado, resultaram em maior crescimento (massa fresca) dos calos. A partir destes calos, foram obtidas suspensões celulares, cuja dinâmica de crescimento e acúmulo de metabólitos foi estudado. Os resultados obtidos deverão ser utilizados para a caracterização de rotas biosintéticas em culturas in vitro em P. solmsianum. Palavras-chave: calogênese, morfogênese, pariparoba, reguladores de crescimento, suspensões celulares ABSTRACT -(In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)).Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l -1 ) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l -1 2,4-D and 2 mg.l -1 BA supplemented medium, and in the absence of activated charcoal, showed the highest calli fresh matter increment. From a callus culture, cell suspension cultures were established and their growth and metabolite accumulation studied. The achieved results may be useful for further characterization of the activated secondary metabolites pathways in in vitro systems of P. solmsianum.

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Section: Introductionmentioning

confidence: 99%

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

et al. 2009

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“…We have conducted some studies with this plant (leaves) at our laboratories, and confirmed that fractions and some pure compounds (lignans) exhibit pronounced antibacterial and antifungal properties [13,14].…”

Section: Introductionmentioning

confidence: 65%

“…Hexane (3.3 g) and ethyl acetate (6.6 g) fractions were fractionated on silica gel column chromatography and eluted as previously described [14] to give pure conocarpan (686 mg) and orientin (765 mg), respectively, together with other compounds not studied here. The identification of the isolated compounds was performed by direct comparison with authentic samples previously described for this plant and spectral data [14].…”

Section: Preparation Of Extract Fractions and Isolation Of Compoundsmentioning

confidence: 99%

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Antinociceptive properties of conocarpan and orientin obtained from Piper solmsianum C. DC. var. solmsianum (Piperaceae)

et al. 2010

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The antinociceptive properties of some fractions and two pure compounds, conocarpan and orientin, obtained from P. solmsianum leaves were investigated in several models of pain in mice. The results indicated that this plant exhibits a promising antinociceptive profile, as it produces active principles which are several times more active than some reference drugs used for comparison. The main compound tested, orientin, caused potent and dose-dependent effects against acetic acid-induced writhing and capsaicin- and glutamate-induced nociception, being more effective against the first one, with an ID(50) value of 6.5 mg/kg (14.5 micromol/kg). Orientin was about 20-fold more potent than acetylsalicylic acid and 3.5-fold more active than indomethacin. The antinociceptive effects of this plant may be attributed, at least partially, to the presence of conocarpan and, in particular, to the flavonoid orientin.

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“…The minimum inhibitory concentration (MIC) was determined by the agar dilution method, according to reported procedures (Campos et al, 2007), which was carried out on slants (1 mL). Stock solutions of ethyl acetate III (EAIII) in dimethylsulfoxide (DMSO) was diluted to give serial two-fold dilutions, which were added to medium (MHA), resulting in concentrations ranging from 0.010 to 10 mg/mL.…”

Section: Quantitative Antimicrobial Evaluation In Vitromentioning

confidence: 99%

Effects of the topical application of an ethyl acetate fraction from Vernonia scorpioides on excisional wounds infected with Staphylococcus aureus in rats

Kreuger¹,

Farias²,

Moreira³

et al. 2012

Rev. bras. farmacogn.

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Abstract:We investigated the healing process on excisional wounds infected with Staphylococcus aureus in rats, treated with 50 μL of ethyl acetate III from Vernonia scorpioides (Lam.) Pers., Asteraceae, rifamycin diethylamide B 25 mg, or saline. The lesions were measured daily and after seven days were surgically removed and histologically processed. The results indicate a favorable action of the EAIII, demonstrated by the increased wound contraction, smaller area of necrotic tissue, good development of granulation tissue, extensive extracellular matrix deposition and epithelial regeneration. This sub-fraction was phytochemically investigated in parallel studies, revealing the presence of sesquiterpene lactones (glaucolides and hirsutinolides) such as diacethylpiptocarphol and related hirsutinolides, flavonoids and cinnamic acid derivatives and also a new polyacetylene, which have been previously published. Results support the effectiveness of V. scorpioides antimicrobial activity in infected wound healing in rats.

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Antibacterial Activity of Extract, Fractions and Four Compounds Extracted from Piper solmsianum C. DC. var. solmsianum (Piperaceae)

Cited by 15 publications

References 29 publications

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

Antinociceptive properties of conocarpan and orientin obtained from Piper solmsianum C. DC. var. solmsianum (Piperaceae)

Effects of the topical application of an ethyl acetate fraction from Vernonia scorpioides on excisional wounds infected with Staphylococcus aureus in rats

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