Antibacterial Activity of Lidah Mertua (Sansevieria Trifasciata Prain.) Leaves Extract on Escherichia coli and Staphylococcus aureus

Abstract

BACKGROUND: Infection is the most common diseases in developing country, including Indonesia. Bacteria that often causes infection is Escherichia coli and Staphylococcus aureus. One of the traditional plants that can be used as an antibacterial is lidah mertua.
 AIM: The purpose of this study was to find out the profile of chemical compounds by thin layer chromatography method and determine the antibacterial activity of Lidah Mertua leaves by in vitro.
 METHODS: This research conducted an experimentally using non-polar, semipolar, and polar as solvents to get extract against E. coli and S. aureus as bacterial testing. The antibacterial activity using agar diffusion method to get minimum inhibitory concentration (MIC).
 RESULTS: The result of the research on thin layer chromatography showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The data obtained were tabulated and analysed descriptively. The antibacterial activity show that n-hexane extract does not provide inhibitory activity. MIC value show that aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 50 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.50 mm and 8.20 mm and methanol extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus at concentration 12.5 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.46 mm and 8.32 mm.
 CONCLUSION: The profile of chemical compounds by thin layer chromatography method showed that the compounds contained in the Lidah Mertua leaves were polifenol, steroids and alkaloids. The antibacterial activity show that n-hexane extract does not provide inhibitory activity, but aethyl acetate extract of lidah mertua leaves inhibited the growth of E. coli and S. aureus.

Estudo anatômico e histoquímico das partes vegetativas de Sansevieria trifasciata Prain

“…A Figura 4 exibe secções transversais da folha submetidas à caracterização histoquímica, observando-se na Figura 4A a folha sem adição de nenhum reagente. Alcaloides (Figura 4B) e compostos fenólicos (Figura 4C) foram observados no parênquima fundamental, sendo esses compostos referidos anteriormente em estudos fitoquímicos (Anbu et al, 2009;Febriani et al, 2019;Umoh et al, 2020). Compostos lipofílicos foram evidenciados na epiderme da espécie, nas células do estômato (Figura 4D) e no parênquima fundamental (Figura 4E).…”

“…Na medicina popular, na África do Sul e na América tropical é utilizada no tratamento de picadas de cobra, dor de ouvido, inchaço, furúnculo e febre (Antunes et al, 2003;Anbu et al, 2009). Na Indonésia, a infusão das folhas é utilizada no tratamento da gripe, tosse, inflamação do trato respiratório, problemas urinários, diarreia, úlceras e fertilizante de cabelo (Febriani et al, 2019). No Brasil, a maceração das folhas em álcool é empregada no tratamento de reumatismo (Machado, 2003).…”

“…Utami (2018), avaliando o extrato etanólico das folhas de S. trifasciata em células T-47D in vitro, descreveram uma citotoxicidade moderada, entretanto mostrou-se promissora para quimioprevenção por diminuir a viabilidade dessas células. Destaca-se que o extrato metanólico das folhas da espécie exibiu atividade antibacteriana contra Escherichia coli e Staphylococcus aureus (Febriani et al, 2019), o extrato etanólico mostrou eficácia contra Pseudomonas aeruginosa (Dewatisari et al, 2021). Anbu et al (2009), avaliando extratos etanólico e aquoso das folhas, comprovaram uma leve atividade analgésica dose-dependente, sendo o extrato aquoso mais eficaz.…”

Antibacterial Properties of Ethanolic Extracts Obtained From Leaves of Some Plants Belonging to the Sansevieria Thunb. Genus Against Acinetobacter Baumannii Strain

“…Minimum inhibitory concentration (MIC) values showed that ethyl acetate extract of S. trifasciata leaves inhibited the growth of E. coli and S. aureus at concentrations 50 mg/mL and 25 mg/mL with diameters of inhibition zone was 8.50 mm and 8.20 mm and methanol extract of S. trifasciata leaves inhibited the growth of E. coli and S. aureus at concentration 12.5 mg/mL and 25 mg/mL with diameters of inhibition zone is 8.46 mm and 8.32 mm. The antibacterial activity revealed that n-hexane extract did not provide inhibitory activity, but ethyl acetate extract of S. trifasciata leaves inhibited the growth of E. coli and S. aureus [15].…”

“…For example, Febriani and co-workers (2019) found the profile of chemical compounds by thin layer chromatography method and determine the antibacterial activity of Lidah Mertua (Sansevieria trifasciata Prain.) leaves in vitro [15]. The result of the research on thin-layer chromatography showed that the compounds contained in the S. trifasciata leaves were polyphenols, steroids, and alkaloids.…”