Antibacterial Activity of Light-Activated Silicone Containing Methylene Blue and Gold Nanoparticles of Different Sizes

Perni, Stefano; Piccirillo, Clara; Kafizas, Andreas; Uppal, Madeeha A.; Pratten, Jonathan; Wilson, Martin; Parkin, Ivan P.

doi:10.1007/s10876-010-0319-5

Cited by 70 publications

(120 citation statements)

References 27 publications

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“…In our study, the presence of 16n m GNPs did not produce any enhancement; on the other hand, the antibacterial activity of PPIX in the presence of GNPs was reduced. This result is in good agreement with the result obtained by Perni et al (2010). They investigated the antimicrobial activity of light-activated silicon containing methylene blue (MB) and GNPs of different sizes.…”

Section: Discussionsupporting

confidence: 88%

Influence of hydrogen peroxide or gold nanoparticles in protoporphyrin IX mediated antimicrobial photodynamic therapy on Staphylococcus aureus

Awad¹,

rasekaran²,

Shanmugam³

et al. 2013

Afr. J. Microbiol. Res.

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Antimicrobial photodynamic therapy (APDT) has been considered to treat skin diseases infected by drug resistant microorganisms. In this work, attempts were made to study the effectiveness of APDT with protoporphyrin IX (PPIX) in the presence of gold nanoparticles (GNPs) or hydrogen peroxide (H 2 O 2 ) against the human bacterial pathogen, Staphylococcus aureus. To photoactivate the PPIX, xenon light source was used at 626 nm. The bactericidal effect was analyzed by standard plate counting method. Attempts were also made to study whether fluorescence spectroscopy can be used to characterize the damage at protein level. Results of the study revealed that PPIX with H 2 O 2 has showed higher bactericidal effect than that of PPIX alone and PPIX with GNPs. From fluorescence spectroscopic characterization it was found that protein damage is one of the reasons of bactericidal effect as there is a considerable change in the intensity of emission and fluorescence lifetime of tryptophan present in the microorganism between pre and post APDT.

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Section: Discussionsupporting

confidence: 88%

Influence of hydrogen peroxide or gold nanoparticles in protoporphyrin IX mediated antimicrobial photodynamic therapy on Staphylococcus aureus

Awad¹,

rasekaran²,

Shanmugam³

et al. 2013

Afr. J. Microbiol. Res.

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show abstract

“…Existing standards rely upon bulk measurements, such as EDS and XPS, in addition to estimating the surface concentration through functional testing, however using these methods means the precise surface composition would remain unknown. [17][18][19][20][21] The analysis technique detailed herein, provides direct imaging and facilitates quantitative analysis of these materials. Quantification of precise nanoparticle concentrations within the polymer is possible, when the fluorescence images are evaluated against known concentrations of the fluorescent material.…”

Section: Representative Resultsmentioning

confidence: 99%

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Crick

Noimark

Peveler

et al. 2016

JoVE

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The fabrication of polymer-nanoparticle composites is extremely important in the development of many functional materials. Identifying the precise composition of these materials is essential, especially in the design of surface catalysts, where the surface concentration of the active component determines the activity of the material. Antimicrobial materials which utilize nanoparticles are a particular focus of this technology. Recently swell encapsulation has emerged as a technique for inserting antimicrobial nanoparticles into a host polymer matrix. Swell encapsulation provides the advantage of localizing the incorporation to the external surfaces of materials, which act as the active sites of these materials. However, quantification of this nanoparticle uptake is challenging. Previous studies explore the link between antimicrobial activity and surface concentration of the active component, but this is not directly visualized. Here we show a reliable method to monitor the incorporation of nanoparticles into a polymer host matrix via swell encapsulation. We show that the surface concentration of CdSe/ZnS nanoparticles can be accurately visualized through cross-sectional fluorescence imaging. Using this method, we can quantify the uptake of nanoparticles via swell encapsulation and measure the surface concentration of encapsulated particles, which is key in optimizing the activity of functional materials. Video LinkThe video component of this article can be found at

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“…When the polymer is removedf rom the solution, the solvente vaporates and the polymers hrinks to its earlier shape, which leaves the antimicrobial agentint he polymer (Figure 4). This methodw as used by Perni et al [43] to incorporate methylene blue/AuNPs into silicone. Later,O zkan et al [44] developed light-activated PDMSt hat contained crystal violet (12)a nd methylene blue (13).…”

Section: Swell-encapsulation Methodsmentioning

confidence: 99%

Recent Developments in the Preparation of Silicones with Antimicrobial Properties

Kottmann

Mejía

Hémery

et al. 2017

Chemistry An Asian Journal

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This Focus Review describes state-of-the-art methods for the preparation of antimicrobials ilicones. Given the diversity of antimicrobiala ctivity and their mechanisms, the performance of these materials is highly dependento nt he characteristics of the polymeric matrix. Therefore, different synthetic routes have been developed, such as 1) physical treatments,2 )chemical transformations, and 3) copolymerization. This classification is not exclusive,s os ome products belong to more than one class. Herein, we attemptt op resent ah andy overview of the development of antimicrobial silicones, their most important application fields, the most relevant antimicrobiala ssays,a nd, as the title suggests, an overview of the most relevant preparation methods.

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Antibacterial Activity of Light-Activated Silicone Containing Methylene Blue and Gold Nanoparticles of Different Sizes

Cited by 70 publications

References 27 publications

Influence of hydrogen peroxide or gold nanoparticles in protoporphyrin IX mediated antimicrobial photodynamic therapy on Staphylococcus aureus

Influence of hydrogen peroxide or gold nanoparticles in protoporphyrin IX mediated antimicrobial photodynamic therapy on Staphylococcus aureus

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Recent Developments in the Preparation of Silicones with Antimicrobial Properties

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