Antibacterial activity of pH‐sensitive genipin cross‐linked chitosan/poly(ethylene glycol)/silver nanocomposites

Abstract

Novel nanocomposites consisting of genipin cross‐linked chitosan (GC), poly(ethylene glycol) (PEG), and silver nanoparticles were prepared for such biomedical applications as the wound‐healing materials. Various amounts of silver nanoparticles were dispersed in the GC/PEG hydrogel matrix without severe aggregation. The effects of composition and silver nanoparticles on the physico‐chemical properties of samples were evaluated by infrared analysis, contact angle measurements, and swelling tests. The GC/PEG/Ag nanocomposite showed a pH‐sensitive swelling behavior. The surface hydrophilicity of GC/PEG/Ag nanocomposites was improved with the increase of silver nanoparticle content. L929 cell attachment was improved in the presence of silver nanoparticles. The antimicrobial function was assessed for the GC/PEG/Ag nanocomposites containing the silver content over 100 ppm. The silver nanoparticles had the dual functions of reinforcing structural stability and enhancing antimicrobial activity of GC/PEG/Ag nanocomposites. Copyright © 2010 John Wiley & Sons, Ltd.

Nanomaterials for application in wound Healing: current state-of-the-art and future perspectives

“…The produced AgNPs minimised the scars, reepithelialised the epidermis, accelerated wound healing as well as the wound contraction rate, and downregulated the level of IL-6, IL-1β, and TNF-α (Table 4) [254]. Genipin (a compound prepared from geniposide, which is extracted from Penicillium nigricans, a slow-growing fungus) when crosslinked with chitosan, PEG, and AgNPs enhanced wound healing with higher antibacterial activity (Table 4) [255]. In Wistar Albino male rats, AgNPs with Coleus forskohlii root extract was effective in healing full thickness excision wounds [168].…”

Biomaterials and Nanotherapeutics for Enhancing Skin Wound Healing

“…Genipin is prepared from geniposide by using the enzyme β-glucosidase, which is extracted from Penicillium nigricans . Genipin cross-linked with chitosan along with PEG and silver NPs are blended into a nanocomposite for enhanced wound healing and high antimicrobial activity (Liu et al, 2012). The formulated silver NPs using Coleus forskohlii root extract has been shown to be effective in healing full-thickness excision wounds in albino Wistar male rats (Naraginti et al, 2016).…”

Semi-IPN Chitosan/PEG Microspheres and Films for Biomedical Applications: Characterization and Sustained Release Optimization

“…Chemical cross-linking is a highly versatile method to create and modify chitosan structures, where properties, such as mechanical, thermal, and chemical stabilities, can be improved. Tripolyphosphate, glutaraldeyhde, genipin, and tartaric acid are used as cross-linkers for chitosan-based materials. Polyethylene glycol (PEG) has been approved by the FDA for a wide range of biomedical materials.…”