In the present study, the antimicrobial activities of methanol extracts obtained from the flowers of Muscari neglectum, Punica granatum, Tussilago farfara, Taraxacum officinale, Wisteria sinensis, Syringa vulgaris, Cyclamen coum, Chaenomeles speciosa, Ornithogalum umbellatum, Allium siculum, Viburnum tinus and Eryngium bithynicum were evaluated. The flower extracts with methanol were prepared in soxhlet device. The evaluation process of the antimicrobial activities was performed using the disc diffusion method. The highest antimicrobial activity has been detected at the strain of S. aureus. The detected inhibition zone diameters of the flower extracts were evaluated to be 15.5 mm for V. tinus, 15 mm for E. bithynicum and 14.5 mm for P. granatum. It has also been found that the flower extracts of C. coum and M. neglectum only effect the C. albicans strain. The flowers of P. granatum has been found to be effective on all the test microorganisms. The extracts obtained from W. sinensis, T. farfara, T. officinale and O. umbellatum have no antimicrobial effect on any test microorganisms. It was found that 7 of the 12 plant species used in the study showed antimicrobial activity. In our study, it was observed that the extracts obtained from flowers were also effective in inhibiting microorganisms. To our knowledge, this is the first study to elucidate the antimicrobial activity of E. bithynicum, V. tinus and A. siculum's flowers and our results how that the flowers of these species have the potential that can be used as antimicrobial natural sources.