Antibacterial Activity of Romanian Propolis against Staphylococcus aureus Isolated from Dogs with Superficial Pyoderma: In Vitro Test

Abstract

Staphylococcal infection treatment in dogs is frequently associated with adverse side effects, high costs, prolonged treatment, and resistant strain selection. Staphylococcus aureus is the most frequently isolated staphylococci in cases of canine superficial pyoderma. The number of Staphylococcus strains to exhibit primary resistance to various drugs in vitro is increasing. Propolis has a diverse chemical composition and well-known therapeutic properties against bacterial infections. The current investigation evaluated in vitro the antimicrobial activity of the commercial allopathic antimicrobials, Romanian propolis ethanolic extracts, against clinical Staphylococcus aureus strains isolated from superficial dermatitis clinical samples in dogs and two reference strains: Staphylococcus aureus ATCC 25923 and Staphylococcus aureus ATCC 43300, as the MSSA and MRSA positive controls, respectively, in western Romania. We used the microdilution broth technique to evaluate the susceptibility profile of the bacteria. The minimum inhibitory concentration (MIC) of the Romanian propolis ethanolic extract ranged from 6 to 10 μg/mL for all isolates, determined by the broth microdilution method. The MICs of ethanolic Romanian propolis extracts had a pronounced antibacterial activity. These results indicate that propolis can potentially be used and recommended for in vivo experiments as a promising therapeutic agent against Staphylococcus aureus infections in superficial dermatitis of dogs.

Romanian Bee Product Analysis: Chemical Composition, Antimicrobial Activity, and Molecular Docking Insights

“…Similarly, Degy et al (2022) demonstrated that Romanian ethanolic propolis had antimicrobial activity against S. aureus with MIC values between 6 and 10 µg/mL [44]. According to the literature, the antimicrobial activity of propolis collected from three European countries against Gram-negative strains was moderate, with MIC values between 0.6 and 5 mg/mL for E. coli and P. aeruginosa; the Irish propolis was the most efficient [11].…”

“…Our previous study describes the methods used by Dégi et al [44] and Obistioiu et al [45]. The concentrations tested were selected based on prior research and a literature search to cover a broad range of concentrations and find possible MIC values [4,8,19,45].…”

Comparación del tratamiento de fitoterapia vs tratamiento convencional de otitis bacteriana “cocos” en perros

“…Tradicionalmente, el tratamiento convencional de la otitis bacteriana en perros ha implicado el uso de antibióticos y medicamentos tópicos recetados por un médico veterinario. Sin embargo, en los últimos años, la fitoterapia ha ganado interés como una posible alternativa o complemento al tratamiento convencional (3). La fitoterapia, según Nabi et al (4) utiliza compuestos fitoquímicos naturales presentes en plantas con propiedades medicinales que se han empleado durante siglos en la prevención y tratamiento de diversas enfermedades.…”

The Influence of Body Weight on Semen Parameters in Apis mellifera Drones

“…Honey bees are among the most important pollinators for natural and agricultural ecosystems, with an estimated 70% of agricultural crops dependent on their pollination [1][2][3][4]. In addition to their major role as pollinators, honey bees provide bee products of particular importance in human and animal therapy [5][6][7][8].…”