Antibacterial and antibiofilm activity of bacterially reduced graphene oxide against some MDR bacterial pathogens isolated from urinary tract infections

Almamad, Muktad Fadel; Aldujaili, Nawfal Hussein

doi:10.1063/5.0066994

Cited by 1 publication

(1 citation statement)

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“…Such a spread of colistin resistance represents a major challenge to public health given that colistin is usually used to treat infections caused by multidrug-resistant (MDR) bacteria, which can complicate the therapeutic options available for clinicians to manage patients. To combat this threat, it is crucial to develop better alternative agents that can reduce the resistance burden and enhance better antimicrobial activity [7].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Physicochemical, Anticancer, Antimicrobial, and Biofilm Activities of N-Doped Graphene

et al. 2022

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Nanomedicine has been used as a precise treatment for many diseases. The advantage of using nanodrugs is that they have more permeability and less toxicity to cells, which enhances the drug delivery system. Graphene is well known for its potential biological applications in drug, food, and pharma industries. This study aimed to assess the productivity and potentiality of nitrogen-doped graphene (NDG) and to evaluate their anticancer, antimicrobial, and biofilm inhibition activity. Nitrogen-doped graphene was synthesized by using a one-pot facile synthesis of NDG, wherein the NDG was prepared by the reduction of graphene oxide (GO) in the presence of hydrazine hydrate as a reducing agent, while ammonium hydroxide was used as a source of nitrogen on the surface of graphene. As-synthesized NDG was characterized by various characterization techniques such as UV-Vis, FT-IR, XRD, XPS, TEM, and N2 sorption studies analysis. Antimicrobial, anticancer, and biofilm inhibition assays were performed by standard protocols. N-doped graphene (NDG) showed better activity against Gram-positive bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), Bacillus subtillis, Streptococcus pneumoniae, and Streptococcus mutans (p ≤ 0.05), whereas there was no activity against Gram-negative strains in Klebsiella pneumoniae and Pseudomonas aeruginosa. Biofilm inhibition was also improved with NDG compared to the standard ampicillin. NDG showed better results in both MCF-7 and Hela cell lines with IC50 of 27.15 µg/mL and 30.85 µg/mL, respectively. In conclusion, NDG has the best ability for use as a biomolecule, and research studies focusing on proteomics, metabolomics, and in vivo studies are needed to increase the impact of NDG in the drug and pharma industry.

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Section: Introductionmentioning

confidence: 99%