The development of multi drug resistance in human and plant pathogens, reconsidering the traditional medicines as antibacterial source and presence of promising phytochemicals in leaf and fruit rind of T. peruviana (S) prompted the authors to take up the antibacterial evaluation of different extracts. Antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of various extracts of T. peruviana (S) were measured using methods of National Committee for Clinical Laboratory Standards (NCCLS).The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) spectrophotometric determination of active extracts has found that the MICs of all the active extracts lies between the range of 250 µg to 1250 µg and bactericidal concentration in a range of 500 to 1250 µg. The percentage of inhibition was analysed, where in among all the extracts tested against B. subtilis and E. coli, fruit upper liquid (FUL) exhibited highest inhibition percentage of 33.75% and 30.31 % at 500 µg/ml respectively. Similarly, fruit hexane (FH) extract has the highest inhibition of 15.60% against B. cereus at 750 µg/ml. The Xanthomonas sp. was susceptible to leaf chloroform (LC) with 32.29% of inhibition at 1000 µg/ ml. The activity index and total antimicrobial activity indicates the antibacterial action of extracts.The present investigations have revealed that among the extracts, the fruit rind extracts have most prominent inhibition abilities against tested bacteria, which are validating the use of this plant in traditional system of medicine and this is the first report of exploration of above extracts for their antibacterial activities against B. subtilis, B. cereus, E. coli and Xanthomonas sp.