Thevetia peruviana is a medicinal plant used in the treatment of external wounds, infected area, ring worms, tumours etc. in traditional system of medicine. The aim of the study was to evaluate the wound healing potentials of T. peruviana leaves hexane (LH) and fruit rind (FW) water extracts and to prove the folkloric claims. The antimicrobial, antioxidant and anti-inflammatory potentials could be important strategies in defining potent wound healing drug. Based on these approaches the current study was designed using incision, excision and dead space wound models with the biochemical, antioxidant enzymes and inflammatory marker analysis. The fruit rind water extract showed highest WBS of 1133 ± 111.4 g. The extracts in excision model retrieved the excised wound i.e. complete healing of wound at day 14. The hydroxyproline content of FW and LH treated dry granuloma tissue was increased to 65.73 ± 3.2 mg/g and 53.66 ± 0.38 mg/g, accompanied by elevations of hexosamine and hexauronic acid with upregulation of GSH, catalase, SOD, peroxidase and the down regulation of the inflammatory marker (NO) and oxidative stress marker (LPO) in wet granulation tissue was documented. Conclusively, both the extracts showed enhanced WBS, rate of wound contraction, skin collagen tissue development, and early epithelisation. Therapeutic wound healing effect was further proven by reduced free radicals and inflammatory makers associated with enhanced antioxidants and connective tissue with histological evidence of more collagen formation. The present research could establish T. peruviana as potential source of effective wound healing drugs.

The development of multi drug resistance in human and plant pathogens, reconsidering the traditional medicines as antibacterial source and presence of promising phytochemicals in leaf and fruit rind of T. peruviana (S) prompted the authors to take up the antibacterial evaluation of different extracts. Antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of various extracts of T. peruviana (S) were measured using methods of National Committee for Clinical Laboratory Standards (NCCLS).The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) spectrophotometric determination of active extracts has found that the MICs of all the active extracts lies between the range of 250 µg to 1250 µg and bactericidal concentration in a range of 500 to 1250 µg. The percentage of inhibition was analysed, where in among all the extracts tested against B. subtilis and E. coli, fruit upper liquid (FUL) exhibited highest inhibition percentage of 33.75% and 30.31 % at 500 µg/ml respectively. Similarly, fruit hexane (FH) extract has the highest inhibition of 15.60% against B. cereus at 750 µg/ml. The Xanthomonas sp. was susceptible to leaf chloroform (LC) with 32.29% of inhibition at 1000 µg/ ml. The activity index and total antimicrobial activity indicates the antibacterial action of extracts.The present investigations have revealed that among the extracts, the fruit rind extracts have most prominent inhibition abilities against tested bacteria, which are validating the use of this plant in traditional system of medicine and this is the first report of exploration of above extracts for their antibacterial activities against B. subtilis, B. cereus, E. coli and Xanthomonas sp.

A research was conducted to evaluate the suitability of soils of different land unit for cereal crops productions at Parbatipur VDC, Chitwan, Nepal with the integrated use of Geographical Information system (GIS) and Multi-criteria evaluation (MCE).Existing geodatabase information from National Land Use Project (NLUP) was used as data for this research. The database was used to derive crop suitability using vector based index model in GIS along with multi-criteria analysis. The research revealed that for rice cultivation, 1.06 %( 10.70ha) was highly suitable, 51.54 %( 515.89ha) was moderately suitable, 3.39% (33.987 ha) was marginally suitable but 28.28 %( 283.11 ha) was not suitable whereas for wheat cultivation, 7.6 %( 76.73ha) was highly suitable, 44.91% (449.53 ha) was moderately suitable and 4.6% (46.26ha) was marginally suitable, but 27.15% (27.14ha) was not suitable. Similarly, for maize cultivation, 12.07% (120.83ha) was highly suitable, 40.19% (402.25ha) was moderately suitable and 0.53% (5.37ha) was marginally suitable, but 31.54% (315.72ha) was not suitable for maize production. Suitability data of this VDC shows that maize and wheat was highly suitable than rice cultivation. But, the area not suitable for rice, wheat and maize must be allocated for alternative uses to achieve optimum productions and further there must be new research for alternative crops in VDC. Hence, this research is useful to farmers and VDC level planners for optimum crops production.

Solanum pubescens Willd is an essential ethnomedicinal plant in treatment of hemorrhoids, inflammation, cancer, whooping cough, rheumatoid arthritis, liver disorders, diarrhea, headache, menstrual pain, tuberculosis, ulcers, and belongs to Southern states of India. Henceforth, to validate its medicinal uses concerning the inflammation management the current study aims to evaluate the acute toxicity, in vitro antioxidant efficacy and in vivo anti-inflammatory action of hexane and chloroform extracts of S. pubescens fruits and stem in carrageenan and cotton pellet-induced inflammation in rats. The investigations revealed that the S. pubescens fruit essential oil (SPFO), S. pubescens fruit chloroform extract (SPFC), S. pubescens stem hexane extract (SPSH) and S. pubescens stem chloroform extract (SPSC) demonstrated substantial 2,2diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radicles (OH •), superoxide anion radicles (SOR), nitric oxide radicles (NOR), 2,4,6-tripyridyl-s-triazine (TPTZ) radicle scavenging effect, lipid peroxidation inhibition, total reducing power and total antioxidant activity. The extracts treatment (200 mg/ kg) exhibited significant in vivo anti-inflammatory impact by inhibiting carrageenan-induced paw edema and the weight of cotton pellet-induced granuloma in rats. The current observations might be valuable in strengthening the ethnomedicinal use and considering S. pubescens as a pool of novel anti-inflammatory agents.

Background: Solanum pubescens Willd, growing wild in the hills of Rayadurg jurisdiction of Southwestern Andhra Pradesh, has gained significant attention among researchers for its diverse folkloric applications, existence of novel phytochemicals and leaf extracts which hold great medicinal promises. To date, the S. pubescens fruit’s essential oil (SPO) has never been investigated. Methods: The current research has been focused to evaluate the chemical composition of S. pubescens fruit essential oil through Gas Chromatography–Mass Spectrometry (GC-MS), followed by the investigation of antibacterial, antifungal, anti-inflammatory, analgesic and wound healing activities in appropriate models to uncover its biological potentials. Extraction of (Solanopuboil/SPO) from the fresh unripe fruits of Solanum pubescens was carried out in Buchner funnel and Whatman no.10 filter paper and concentrated at 40oC using a rotary evaporator. The metabolic profiling of SPO was analysed by GC–MS technique. The MIC, MBC, activity index, and total antimicrobial activity of SPO were evaluated using standard procedures. Anti-inflammatory activity of SPO was screened using Carrageenan induced paw oedema and Cotton pellet-induced granuloma. Tail immersion test, Acetic acid writhing response and Formalin paw lick test was performed in rats in order to examine the analgesic activity of SPO. Wound healing activity of SPO was investigated by performing the incision wound model, Excision wound model and Dead space wound model in rats. Results: The SPO displayed a constant degree of antimicrobial activity against B. cereus, B. subtilis, E. coli, A. niger, A. fumigatus and C. albicans with significant anti-inflammatory and analgesic activities. Also, a prominent wound healing potential of it was observed in excision, incision and dead space wound models with considerable elevation in granulation tissue hydroxyproline, hexuronic acid and hexosamine content in association with remarkable regulation of anti-inflammatory and antioxidant markers i.e., Lipid peroxidase (LPO), Nitric Oxide (NO), Superoxide dismutase (SOD), Glutathione (GSH), Catalase (CAT), Glutathione Peroxidase (GPx). Conclusion: These findings strongly validate the therapeutic potential of S. pubescens fruit essential oil in antimicrobial and anti-inflammatory mediated wound healing and suggests its promising application as valuable and novel indigenous leads in the food and pharmaceutical industries. To the best of our knowledge, this is the first-ever investigatory report on the systematic phytochemical and therapeutic examination of S. pubescens fruit essential oil.