Wound healing potentials of Thevetia peruviana : Antioxidants and inflammatory markers criteria

Abstract

Thevetia peruviana is a medicinal plant used in the treatment of external wounds, infected area, ring worms, tumours etc. in traditional system of medicine. The aim of the study was to evaluate the wound healing potentials of T. peruviana leaves hexane (LH) and fruit rind (FW) water extracts and to prove the folkloric claims. The antimicrobial, antioxidant and anti-inflammatory potentials could be important strategies in defining potent wound healing drug. Based on these approaches the current study was designed using incision, excision and dead space wound models with the biochemical, antioxidant enzymes and inflammatory marker analysis. The fruit rind water extract showed highest WBS of 1133 ± 111.4 g. The extracts in excision model retrieved the excised wound i.e. complete healing of wound at day 14. The hydroxyproline content of FW and LH treated dry granuloma tissue was increased to 65.73 ± 3.2 mg/g and 53.66 ± 0.38 mg/g, accompanied by elevations of hexosamine and hexauronic acid with upregulation of GSH, catalase, SOD, peroxidase and the down regulation of the inflammatory marker (NO) and oxidative stress marker (LPO) in wet granulation tissue was documented. Conclusively, both the extracts showed enhanced WBS, rate of wound contraction, skin collagen tissue development, and early epithelisation. Therapeutic wound healing effect was further proven by reduced free radicals and inflammatory makers associated with enhanced antioxidants and connective tissue with histological evidence of more collagen formation. The present research could establish T. peruviana as potential source of effective wound healing drugs.

Ethnopharmacology, phytochemistry, and biotechnological advances of family Apocynaceae: A review

“…Of late, hexane and water extracts of Thevetia peruviana leaves and fruits respectively were evaluated for their wound healing potential (Rahman, Rahman, Haris, & Mahmood, 2017). The activity was studied on incision, excision, and using dead space wound rat models.…”

Antioxidant and anti-inflammatory potentials of Solanum pubescens Willd an ethnomedicinal plant of South Western Andhra Pradesh, India

“…Whereas, the total antioxidant assay model ( Figure 1H) revealed the potency of SPFO with an IC50 of 283.27 ± 0.24 when compared with the respective standards as indicated in Table 1. ROS and RNS factor even though these molecules involve in the accretion of macrophages and settling of inflammatory cells to their respective positions, when surpassed may also be responsible for the delayed salvation of wounds by persuading tissue damage [22]. The inhibition of respective free radicles speak to the capacity of S. pubescens fruit and stem hexane and chloroform extracts enriched with diverse phytochemicals and considerable amount of flavonoids and phenolics to apply the potential antioxidant action and this therapeutic action can be credited to exert profound anti-inflammatory activity, as it is recognized that the free radicle scavenging potentials protects the CNS and ultimately triggers the pain reduction and tissue damage derived from lipid peroxidation at the inflamed site [23,24].…”

“…Furthermore, the study demonstrated unaltered body weight among the extract treated groups compared to control animals showing the nontoxic nature of the S. pubescens extracts. It is noteworthy that the observed anti-inflammatory properties of S. pubescens fruit and stem extracts for both acute and chronic inflammatory models is credited to defensive ability of phytochemicals present essentially flavonoids and its derivatives [10,[12][13][14][15][16]22], most likely regulate through antioxidation action, and proinflammatory molecules production [8,16,[23][24][25]. In addition, the current investigations approves the S. pubescens as potential source of anti-inflammatory agents among Solanum genus as the S. pubescens hexane and chloroform extracts at 200mg/kg demonstrated remarkable activity when compared with the plants like S. aethiopimcum, S. xanthocarpum, S. scabrum and S. lycopersicum have been therapeutically explored for their anti-inflammatory potentials [26,[27][28][29].…”

Antioxidant, anti-inflammatory and wound healing properties of ethanolic extracts of Thevetia peruviana (Pers.) K. Schum

“…In Ayurveda, many prominent scholars have advocated the use of leaves of plant T. peruviana (Karvira) for the treatment of external wounds, infected area, ringworms, inflammation, fever, tumors, skin disorders and in ethnoveterinary use [6,7]. Considering the ethnomedicinal claims and the lack of systematic information on wound healing potentials of T. peruviana leaf and fruit rind, the current study has been called for a thorough exploration of wound curative capabilities, by evaluating the antioxidant, and antiinflammatory means.…”