Antibacterial and antitumor activity of Bogorol B-JX isolated from Brevibacillus laterosporus JX-5

Abstract

Antimicrobial peptides are promising anti-infective agent candidates because they have a broad antimicrobial spectrum and bioactivity and are unlikely to elicit antibiotic resistance. The bogorols represent a new cationic antibiotic peptide and possess great therapeutic potential because of their bioactivity and precise mode of action. Here, we report that Bogorol B-JX (BBJX), a peptide previously isolated from Brevibacillus laterosporus JX-5 by us, has significant antibacterial and antitumor activities in vitro. BBJX was found to inhibit methicillin-resistant Staphylococcus aureus (MRSA) at 2.5 µg/mL with distinct mechanisms of action from those against Bacillus bombyseptieus and Escherichia coli. It penetrates MRSA membrane with little visible destruction and binds to genomic DNA. BBJX could inhibit the proliferation of human histiocytic lymphoma cell line U-937 and ConA-activated spleen cells at 5 µg/mL, but was not cytotoxic to the Jurkat cells, resting spleen cells or differentiated macrophage-like U-937 immunocytes. Moreover, BBJX caused apoptosis of U-937 cells by opening the mitochondrial permeability transition pore and stimulating the production of reactive oxygen species. Taken together, these studies provided basis for future medical application of the bogorols.

Broad‐spectrum cytotoxicity to cancer cells of Brevilaterin C from Brevibacillus laterosporus and its specific mechanism on human epidermal cancer cells

“…And our finding indicated that Brevilaterin C could obviously induce apoptosis and increase ROS levels of A431 cells, in a similar manner to that reported for other pentapeptides against the HL‐60 cell line 35 . Compared with 3 µg/ml of Brevilaterin C, another bacterial AMPs Bogorol B‐JX at 10 µg/ml isolated from the same bacterium B. laterosporus JX‐5 could display a similar effect on ROS generation in U‐937 cells 21 . The mitochondrial electron transport chain is the major source of ROS; moreover, when the function or integrity of the chain is compromised, (e.g., by treatment with uncoupling agents), excessive amounts of ROS are generated 21 .…”

“…Indeed, only one report published by Jiang described the bioactivity of AMPs from B. laterosporus toward human cancer cells. They indicated that Bogorol B‐JX efficiently inhibition proliferation of the human histiocytic lymphoma cell line U‐937 21 . However, there had never addressed a systematic exploration of the anticancer properties of AMPs from B. laterosporus toward different human tumors.…”

“…Compared with 3 µg/ml of Brevilaterin C, another bacterial AMPs Bogorol B‐JX at 10 µg/ml isolated from the same bacterium B. laterosporus JX‐5 could display a similar effect on ROS generation in U‐937 cells 21 . The mitochondrial electron transport chain is the major source of ROS; moreover, when the function or integrity of the chain is compromised, (e.g., by treatment with uncoupling agents), excessive amounts of ROS are generated 21 . Decreased mitochondrial membrane potential is a typical event in the early stage of apoptosis, which occurs before the appearance of nuclear apoptotic characteristics (such as chromatin concentration and DNA breakage).…”

BLB8, an antiviral protein from Brevibacillus laterosporus strain B8, inhibits Tobacco mosaic virus infection by triggering immune response in tobacco

“…Brevibacillus laterosporus , one of the most valuable biocontrol bacterias for its insecticidal activity, 23 has attracted more and more attention recently because of its antibacterial activity 24–27 . Wang et al characterized a novel protein elicitor PeBL1 from B. laterosporus strain A60.…”

Rapid polymerase chain reaction assays for Brevibacillus laterosporus detection

“…The medical interest is related to the synthesis of antibiotics acting against diverse human pathogenic fungi and bacteria, including Candida albicans [2], Escherichia coli, Klebsiella, Pseudomonas, Salmonella, Staphylococcus, and Streptococcus species [3,4]. Antitumor compounds from B. laterosporus have also found specific medical applications [5,6]. In the field of agriculture, the high biocontrol potential of this bacterial species relies on the production of a variety of toxins, enzymes, and other virulence factors acting against phytopathogens and invertebrate pests, including insects, nematodes, and molluscs [1].…”