Antibacterial and cytotoxic efficacy of Nano- Hydroxyapatite Synthesized from Eggshell and Sheep bones bio Waste

Mousavi, Seyyed Mojtaba; Hashemi, Seyyed Alireza; Yousefi, khadijeh; Rao, N. Vijayakameswara; Omidifar, Navid; Gholami, Ahmad; Ghahramani, Yasamin; Chiang, Wei‐Hung; Nezhad, Fatemeh Fallahi; Kalashgrani, Masoomeh Yari

doi:10.21203/rs.3.rs-2195140/v1

Cited by 3 publications

(1 citation statement)

References 40 publications

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“…In Nambiar's research, S.mutans experienced total death after application for 48 hours at a concentration of 20%. 18 Mousavi et al, 21 conducted research on the antibacterial ability of nanohydroxyapatite synthesized from eggshell and sheep bones waste against other gram-positive bacteria, namely S. aureus, and the results showed that nanohydroxyapatite has the ability to kill all bacteria at concentrations 500μg/mL. This difference in concentration is likely due to the antibacterial ability of hydroxyapatite being influenced by size, external surface and type of bacteria.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of nano-hydroxyapatite paste of snakehead fish bone against S. mutans: an in vitro study

Dewi,

Rahmadella,

Hatta

et al. 2024

Padjadjaran J Dent

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Introduction: Caries is the most common oral disease found in society. The prevalence of caries in South Kalimantan is as high as 46.9% in 2018. S.mutans is the causative microorganism in the initial occurrence of caries. Strategy that can be used to prevent caries is by adding nano-hydroxyapatite to the tooth paste. Nano-hydroxyapatite can be obtained from Snakehead (Channa striata) fish bone. Snakehead is a kind of fish that is abundant in Banjarmasin. This study aimed to analyze antibacterial activity of the nano-hydroxyapatite paste from snakehead (Channa striata) fish bone against S.mutans bacteria. Methods: The study consisted of 5 treatment groups: negative control (basic formula), positive control (casein phosphopeptide-amorphous calcium phosphate or CPP-ACP) and three treatment groups (nano-hydroxyapatite paste concentration of 10, 20 and 30%. The paste was made in the formulation of F1, F2, and F3. The antibacterial activity test by measuring MIC and MBC were performed using dilution method. Results: MIC of nano-hydroxyapatite paste was at a concentration of 10% with an average value of the difference absorbance of -0.468. MIC values in the concentration of 10%, 15%, 20% positive and negative control groups had a significant difference. MBC of nano-hydroxyapatite paste was at concentration of 15%. Concentration of 10% and the negative control group showed a significant difference, while concentration of 15% and 20% groups did not show a significant difference. Conclusion: Nano-hydroxyapatite paste from Snakehead fish bone has antibacterial activity in inhibiting and eliminating mutated S.mutans bacteria. The most effective concentration of antibacterial nano-hydroxyapatite paste to prevent caries was 10%. At this concentration, nano-hydroxyapatite can inhibit the growth of S.mutans without killing the bacteria.KEYWORDS fish bone, nano-hydroxyapatite, snakehead, S.mutans

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Section: Discussionmentioning

confidence: 99%

Antibacterial activity of nano-hydroxyapatite paste of snakehead fish bone against S. mutans: an in vitro study

Dewi,

Rahmadella,

Hatta

et al. 2024

Padjadjaran J Dent

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Remineralizing Potential of Natural Hydroxyapatite from Snakehead (Channa striata) Fish Bone on Remineralization of Primary Teeth Enamel: An In Vitro Study

Dewi,

Rahmah,

Wardhana

et al. 2024

European Journal of General Dentistry

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Objective This study aimed to investigate the effect of hydroxyapatite from snakehead (Channa striata) fish bone on the surface hardness of the enamel of the primary teeth. Materials and Methods Twenty-six primary maxillary incisors were mounted on self-cured acrylic resin, divided into two groups, and demineralized. Remineralization was performed using hydroxyapatite paste synthesized from C. striata fish bone by the precipitation method. The case group was subjected to 15% hydroxyapatite paste and subsequently submerged in artificial saliva, while the control group was only exposed to artificial saliva. Enamel hardness was measured by the Vickers hardness tester after 7 days of treatment. The statistical analysis used in this research was an independent t-test. Results The case group had a surface hardness of 356.192 ± 25.218, and the control group had a surface hardness of 269.686 ± 22.931. Statistical tests showed a significant difference between the case and control groups. Conclusion Hydroxyapatite paste from snakehead (C. striata) fishbone stimulates remineralization of primary teeth, as evidenced by an increase in the enamel surface hardness of the teeth.

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Biomass-Based Silica/Calcium Carbonate Nanocomposites for the Adsorptive Removal of Escherichia coli from Aqueous Suspensions

et al. 2023

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The present study deals with the adsorptive removal of Escherichia coli (E. coli) by making use of chitosan-silica/calcium carbonate (CS-SiO2/CaCO3) nanocomposites (NCs) where it was synthesized using the waste eggshells and rice husks occurred by natural sources. The bioadsorbent CS-SiO2/CaCO3 NCs were synthesized by incorporating eggshell-CaCO3 nanoparticles (NPs) and rice husk-SiO2 NPs in chitosan NPs solution. The adsorbents were characterized using HRTEM, BET, DLS, and TGA. The characterization of NCs revealed the formation of adsorbents in the range of 10–50 nm and some structural changes to the spectra of adsorbents before and after the adsorption of E. coli was revealed by the FTIR analysis. Moreover, the adsorption efficiency of E. coli over the adsorbents after 35 min of incubation was about 80% for CS-SiO2/CaCO3 NCs. Further, the kinetics of adsorption studies were observed to be well-fitted to the Langmuir isotherm model with an adsorption capacity of 3.18 × 101 (CFU E. coil per gram of CS-SiO2/CaCO3). From the analysis, the synthesized bioadsorbent demonstrated the potential for ameliorating the inherent risk of pathogens in water.

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Antibacterial and cytotoxic efficacy of Nano- Hydroxyapatite Synthesized from Eggshell and Sheep bones bio Waste

Cited by 3 publications

References 40 publications

Antibacterial activity of nano-hydroxyapatite paste of snakehead fish bone against S. mutans: an in vitro study

Antibacterial activity of nano-hydroxyapatite paste of snakehead fish bone against S. mutans: an in vitro study

Remineralizing Potential of Natural Hydroxyapatite from Snakehead (Channa striata) Fish Bone on Remineralization of Primary Teeth Enamel: An In Vitro Study

Biomass-Based Silica/Calcium Carbonate Nanocomposites for the Adsorptive Removal of Escherichia coli from Aqueous Suspensions

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