Antibacterial and immunomodulatory effects of Pheromonicin-NM on Escherichia coli-challenged bovine mammary epithelial cells

Yang, Guo Xiang; Yuan, Yue; Li, Dan; Duan, Cong; Qiu, Xiao‐Qing; Zou, Yunjing; Zhu, Yaohong; Lauridsen, Charlotte; Wang, Jiufeng

doi:10.1016/j.intimp.2020.106569

Cited by 10 publications

(7 citation statements)

References 26 publications

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“…The cell membrane receptor TLR4 recognizes exogenous stimuli such as lipopolysaccharide from E. coli , and the signal is transmitted through MyD88, activates NF-κB, and then modulates many of the pro-inflammatory cytokines and chemokine transcripts that contribute to the development of mastitis. Previous studies have demonstrated that mRNA expression of TLR4 was upregulated and levels of inflammatory factors such as IL-8 and IL-6 were increased in MAC-T cells after E. coli infestation [ 43 , 44 ]. LPS stimulation of TLR4 induces activated NF-κB and MAPK inflammatory pathways [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Artemisinin on Escherichia coli–Induced Mastitis in Bovine Mammary Epithelial Cells and Mice

Wang

et al. 2022

Veterinary Sciences

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Bovine mastitis is an important disease affecting dairy farming, and it causes large economic losses to the dairy industry. Escherichia coli (E. coli) is considered to be a causative environmental pathogen and frequently enters into mammary glands, causing inflammation. Artemisinin is a highly effective malaria remedy and is not easy to develop drug resistance to. In recent years, other effects of artemisinin (including antitumor, anti-inflammatory, antifungal, etc.) have been increasingly discovered and applied. The current study aimed to investigate whether artemisinin could attenuate E. coli–induced inflammation. Through the E. coli mastitis model in MAC-T cells and mice, the protective effects of artemisinin were analyzed by CCK-8 (Cell Counting Kit-8), Western blot, and RT-qPCR. The results showed that artemisinin reversed the decrease of cell viability and upregulated TLR4 (toll-like receptor 4)/NF-κB (nuclear factor κB) and MAPK (mitogen activated protein kinase)/p38 signaling pathways, as well as restrained the expression of TNF-α, IL-6, and IL-1β mRNA caused by E. coli. Meanwhile, artemisinin also alleviated mammary tissue damage, reduced inflammatory cells’ infiltration, and decreased the levels of inflammatory factors in a mice mastitis model. This study demonstrated that artemisinin alleviated the inflammatory response of mouse mastitis and MAC-T cells induced by E. coli, thus providing a practical approach for the clinical control of mastitis.

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Section: Discussionmentioning

confidence: 99%

Effects of Artemisinin on Escherichia coli–Induced Mastitis in Bovine Mammary Epithelial Cells and Mice

Wang

et al. 2022

Veterinary Sciences

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“…Often, it is caused by the invasion and proliferation of pathogenic microorganisms, physical damage, chemical stimulation and the cow's immune response (1,2,37). However, among these causes, E. coli is the most common culprit (38,39). When E. coli successfully penetrate the mammary gland through the physical barrier of the teat end, it causes an inflammatory response in the mammary gland (38,40).…”

Section: Discussionmentioning

confidence: 99%

“…However, among these causes, E. coli is the most common culprit (38,39). When E. coli successfully penetrate the mammary gland through the physical barrier of the teat end, it causes an inflammatory response in the mammary gland (38,40). This colonization leads to rapid changes in the expression levels of immune-related genes, resulting in the inflammatory process (41,42).…”

Section: Discussionmentioning

confidence: 99%

Differential Expression Profiles of lncRNA Following LPS-Induced Inflammation in Bovine Mammary Epithelial Cells

Wang

Yang

et al. 2021

Front. Vet. Sci.

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Bovine mastitis is an inflammatory response of mammary glands caused by pathogenic microorganisms such as Escherichia coli (E. coli). As a key virulence factor of E. coli, lipopolysaccharide (LPS) triggers innate immune responses via activation of the toll-like-receptor 4 (TLR4) signaling pathway. However, the molecular regulatory network of LPS-induced bovine mastitis has yet to be fully mapped. In this study, bovine mammary epithelial cell lines MAC-T were exposed to LPS for 0, 6 and 12 h to assess the expression profiles of long non-coding RNAs (lncRNAs) using RNA-seq. Differentially expressed lncRNAs (DElncRNAs) were filtered out of the raw data for subsequent analyses. A total of 2,257 lncRNAs, including 210 annotated and 2047 novel lncRNAs were detected in all samples. A large proportion of lncRNAs were present in a high abundance, and 112 DElncRNAs were screened out at different time points. Compared with 0 h, there were 22 up- and 25 down-regulated lncRNAs in the 6 h of post-infection (hpi) group, and 27 up- and 22 down-regulated lncRNAs in the 12 hpi group. Compared with the 6 hpi group, 32 lncRNAs were up-regulated and 25 lncRNAs were down-regulated in the 12 hpi group. These DElncRNAs are involved in the regulation of a variety of immune-related processes including inflammatory responses bMECs exposed to LPS. Furthermore, lncRNA TCONS_00039271 and TCONS_00139850 were respectively significance down- and up-regulated, and their target genes involve in regulating inflammation-related signaling pathways (i.e.,Notch, NF-κB, MAPK, PI3K-Akt and mTOR signaling pathway), thereby regulating the occurrence and development of E. coli mastitis. This study provides a resource for lncRNA research on the molecular regulation of bovine mastitis

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“…Thus, the authors reported the role of colicins as important bacterial factors in inflammation-induced blooms [ 97 ]. The immunomodulatory effect of enterobacteria and their bacteriocins has been investigated [ 98 , 99 ]. According to Pang et al [ 98 ], gut microbiota, including E. coli , suppresses allergic responses in mice and allergic airway disease.…”

Section: Interaction Of Colicins and Microcins With Prokaryotic And E...mentioning

confidence: 99%

Colicins and Microcins Produced by Enterobacteriaceae: Characterization, Mode of Action, and Putative Applications

Marković

Grujović

Koraćević

et al. 2022

IJERPH

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Enterobacteriaceae are widely present in many environments related to humans, including the human body and the food that they consume, from both plant or animal origin. Hence, they are considered relevant members of the gastrointestinal tract microbiota. On the other hand, these bacteria are also recognized as putative pathogens, able to impair human health and, in food, they are considered indicators for the microbiological quality and hygiene status of a production process. Nevertheless, beneficial properties have also been associated with Enterobacteriaceae, such as the ability to synthesize peptides and proteins, which can have a role in the structure of microbial communities. Among these antimicrobial molecules, those with higher molecular mass are called colicins, while those with lower molecular mass are named microcins. In recent years, some studies show an emphasis on molecules that can help control the development of pathogens. However, not enough data are available on this subject, especially related to microcins. Hence, this review gathers and summarizes current knowledge on colicins and microcins, potential usage in the treatment of pathogen-associated diseases and cancer, as well as putative applications in food biotechnology.

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Antibacterial and immunomodulatory effects of Pheromonicin-NM on Escherichia coli-challenged bovine mammary epithelial cells

Cited by 10 publications

References 26 publications

Effects of Artemisinin on Escherichia coli–Induced Mastitis in Bovine Mammary Epithelial Cells and Mice

Effects of Artemisinin on Escherichia coli–Induced Mastitis in Bovine Mammary Epithelial Cells and Mice

Differential Expression Profiles of lncRNA Following LPS-Induced Inflammation in Bovine Mammary Epithelial Cells

Colicins and Microcins Produced by Enterobacteriaceae: Characterization, Mode of Action, and Putative Applications

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