Differential Expression Profiles of lncRNA Following LPS-Induced Inflammation in Bovine Mammary Epithelial Cells

Abstract

Bovine mastitis is an inflammatory response of mammary glands caused by pathogenic microorganisms such as Escherichia coli (E. coli). As a key virulence factor of E. coli, lipopolysaccharide (LPS) triggers innate immune responses via activation of the toll-like-receptor 4 (TLR4) signaling pathway. However, the molecular regulatory network of LPS-induced bovine mastitis has yet to be fully mapped. In this study, bovine mammary epithelial cell lines MAC-T were exposed to LPS for 0, 6 and 12 h to assess the expression profiles of long non-coding RNAs (lncRNAs) using RNA-seq. Differentially expressed lncRNAs (DElncRNAs) were filtered out of the raw data for subsequent analyses. A total of 2,257 lncRNAs, including 210 annotated and 2047 novel lncRNAs were detected in all samples. A large proportion of lncRNAs were present in a high abundance, and 112 DElncRNAs were screened out at different time points. Compared with 0 h, there were 22 up- and 25 down-regulated lncRNAs in the 6 h of post-infection (hpi) group, and 27 up- and 22 down-regulated lncRNAs in the 12 hpi group. Compared with the 6 hpi group, 32 lncRNAs were up-regulated and 25 lncRNAs were down-regulated in the 12 hpi group. These DElncRNAs are involved in the regulation of a variety of immune-related processes including inflammatory responses bMECs exposed to LPS. Furthermore, lncRNA TCONS_00039271 and TCONS_00139850 were respectively significance down- and up-regulated, and their target genes involve in regulating inflammation-related signaling pathways (i.e.,Notch, NF-κB, MAPK, PI3K-Akt and mTOR signaling pathway), thereby regulating the occurrence and development of E. coli mastitis. This study provides a resource for lncRNA research on the molecular regulation of bovine mastitis

Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese

“…LPS is a key virulence factor of Escherichia coli (E. coli) that triggers innate immune responses via activation of the toll-like receptor 4 signaling pathway ( 39 ). To identify, whether E. coli contributes to activating the LPS, the batch cultivation for E. coli was carried out in Luria broth (LB) medium at 37°C with a 2-L working volume.…”

Role of Long Noncoding RNAs in the Regulation of Cellular Immune Response and Inflammatory Diseases

“…Mumtaz et al [ 134 ] identified 112 differentially expressed lncRNAs in goat mammary epithelial cells induced by E. coli and S. aureus . In addition, Wang et al [ 135 ] also found 112 differentially expressed lncRNAs in LPS-treated bovine mammary epithelial cells, and these lncRNAs participated in inflammation-related signal pathways (i.e., the Notch, NF-κB, MAPK, and PI3K-AKT signal pathways). A large amount of the lncRNA information in the present study may provide clues for functional and molecular studies of mammary epithelial cells–bacteria interaction.…”

Genetic Bases and Molecular Breeding of Key Economic Traits in China Dairy Cattle: A Progress Report

“…With the development of molecular biology, biostatistics, molecular genetics and marker-assisted breeding technology in recent years, assisted breeding using experimental methods of molecular biology is becoming a new and feasible breeding method [10,11]. The Molecular breeding of dairy cattle has centered around the balanced breeding concept in which functional traits such as reproduction, body shape, health and longevity [12,13] are incorporated into the breeding program. Identifications of genes and genetic markers relevant to milk production are expected to accelerate genetic analysis and breeding programs [14].…”

“…At present, the research on the factors and pathways related to the regulation of inflammation at the molecular level is increasing. A combination of bioinformatics analysis and mechanistic studies has found by Wang et al that target genes of long non-coding RNAs (lncRNA), tcon_00039271 and tcon_00139850, regulated inflammation-related signaling pathways (Notch, NF-κb, MAPK, PI3K-Akt and mTOR) and affected susceptibility to Escherichia coli-induced mastitis [13]. Furthermore, micro-RNAs, miR-320a, miR-19a, miR-19b, miR-143, miR-205 and miR-24, may be blood biomarkers for Staphylococcus aureus-induced mastitis [22].…”