Antibacterial and synergistic effect of biosynthesized silver nanoparticles using the fungi Tritirachium oryzae W5H with essential oil of Centaurea damascena to enhance conventional antibiotics activity

Abstract

The aims of this study are to synthesis silver nanoparticles (AgNPs) using the culture supernatant of the fungal strain Tritirachium oryzae W5H and to investigate the antibacterial activity of AgNPs individually and in combination with some antibiotics and essential oil of the plant Centaurea damascena. The colour of the filtrate with silver nitrate solution changed to intense brown after 72 h of incubation. The UV-Vis is spectrum shows a surface plasmon resonance (SPR) peak of AgNPs at 425 nm. For confirmation of the presence of AgNPs in the solution, analysis of transmission electron microscopy (TEM) image, scanning electron microscope (SEM) micrograph and SEM-EDS were recorded. The current study showed that AgNPs possess broad spectrum activity with inhibition zone ranged from 12 nm to 22 mm. Notable synergy was seen between AgNPs and either vancomycin, nitrofurantoin, chloramphenicol or tetracycline, against Pseudomonas aeruginosa, with a 2.4-fold to 9-fold, increase in fold area of inhibition (IFA). Similarly, AgNPs with gentamicin, tetracycline, ampicillin, cefotaxime and trimethoprim against E. coli, P. aeruginosa, S. aureus and S. epidermidis had a synergy of 1-fold to 10-folds. In addition, synergy was observed between AgNPs, essential oil (EO) of C. damascena and either amoxicillin, ampicillin, trimethoprim or nitrofurantoin against S. epidermidis by 0.7-fold to 3.7-fold and with trimethoprim against P. aeruginosa, increased the IFA by 1.3-fold. Gentamicin led to the greater enhancement in the IFA up to 9-folds. Moreover, effective synergy was seen between EO of C. damascena and either gentamicin or amoxicillin against E. coli by 11.3-fold and 3.7-fold, respectively, whereas amoxicillin against P. aeruginosa and S. epidermidis by 11.25-fold and 1.8-fold IFA, respectively. The results of this study may lead to the new concepts of antibacterial agents that could contain nanoparticles (NPs) or containing new substances that are likely to have extensive synergy of antibiotics with NPs and essential oils for medicinal plants.

Mycogenic Metal Nanoparticles for the Treatment of Mycobacterioses

“…Most mycogenic AgNPs studied are spherical although some variations, mostly due to the formation of clusters, can also be found [ 41 ]. In most mycogenic processes (those following the method shown in Figure 1 ), the common concentration of the precursor—silver nitrate (AgNO 3 )—is 1 mM [ 48 , 89 , 103 , 106 ]. Within the size variation described, mycosynthesized AgNPs when produced with enzymatic stimulation (from co-cultures) form smaller and more active NPs [ 106 ].…”

“…Combination therapy for mycobacterial infections can increase the potential activity of MNPs, contribute to decrease the effective dose of antibiotics potentiating them; reduce side effects, drug toxicity, and MNPs toxicity; enhance bioavailability; and enhance solubility and retention time [ 103 , 124 ].…”

3,4-Dichlorobenzoic acid biodegradation by the Edwardsiella tarda: Effect of Some Growth Conditions

“…Furthermore, it has been shown repeatedly that degradation patterns of pollutants can differ from single-compound studies and this due to inhibiting effects on the aerobic biodegradation (Tiehm and Fritzsche, 1995;Shawabkeh et al, 2007;Al-Asoufi et al, 2017;Al-Limoun et al, 2019;Qaralleh et al, 2019;Zhao et al, 2010;Zeidan et al, 2013). However, co-metabolic degradation of poorly degradable compounds in the presence of growth substrates can improve overall degradation.…”

An Updated Review on Silver Nanoparticles in Biomedicine

“…Enhanced inhibitory effects on E. coli were reported when using the treatment with AgNPs, Centaurea damascena essential oil and Gentamicin or Amoxicillin. In the case of S. aureus , the most prominent synergistic effect was reported when combining nanoparticles and essential oil with Imipenem [ 172 ]. Pronounced antibacterial effects on both strains were also reported when treated with Vancomycin-loaded AgNPs [ 173 ].…”