Antibacterial and transfection activities of nebulized formulations incorporating long n-alkyl chain silver N-heterocyclic carbene complexes

Mottais, Angélique; Berchel, Mathieu; Gall, Tony Le; Sibiril, Y.; d’Arbonneau, Frédérique; Laurent, Véronique; Jaffrès, Paul‐Alain; Montier, Tristan

doi:10.1016/j.ijpharm.2019.118500

Cited by 19 publications

(9 citation statements)

References 48 publications

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“…The aerosol delivery was performed using an unrestrained whole-body exposure protocol with the PARI LC PLUS nebulizer (PARI, Starnberg, Germany), which has been tested and proven in many clinical trials (Figure 3; see also Figure S1 for more details about the experimental set-up) [8,9]. For each aerosol experiment, 15 to 20 mice were introduced into the exposure box.…”

Section: Aerosol Deliverymentioning

confidence: 99%

“…The main lipidic formulations considered in the present study (F1 to F20, Table 2) were noted as "F# + L" or "F# + C", the letters "L" and "C" referring to the luciferase-encoding (pGM144) and the CFTR-encoding (pCIK-CFTR) pDNA, respectively. 1 Lipid formulation (F#) mixed with the luciferase-encoding pDNA pGM144 (L); 2 in addition to DP5K; 3 cationic lipid/colipid(s) molar ratio; 4 DP5K/pDNA mass ratio; 5 cationic lipid/pDNA charge ratio; 6 in nm; 7 polydispersity index; 8 in mV; 9 relative lung reporter gene expression on day 1 (D1), 7 (D7) and 14/28 (D28) (the minimal luciferase expression being given the value of 1) and classification according to the maximal luciferase expression (MLE) reached (on average on day 1, 7, or 14/28), as follows: Gr1 (inefficient), MLE ≤ 10 3 ; Gr2 (slightly efficient), MLE ≤ 10 4 ; Gr3 (efficient), MLE ≤ 10 5 ; Gr4 (most efficient), MLE ≤ 10 6 RLU/mg protein (Figure 4). For naive mice, the luminescence signal measured was ~10 2 RLU/mg protein.…”

Section: All Lipidic Formulations Were Not Stable When Mixed With Highly Concentrated Pdnamentioning

confidence: 99%

“…Encouraging progress has been achieved supporting further developments in this approach [4,5]. However, the airway delivery route also faces specific obstacles, stimulating research focusing on both the inhalation method [6] and the therapeutic treatment to be delivered [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

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Aerosol-Mediated Non-Viral Lung Gene Therapy: The Potential of Aminoglycoside-Based Cationic Liposomes

et al. 2021

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Aerosol lung gene therapy using non-viral delivery systems represents a credible therapeutic strategy for chronic respiratory diseases, such as cystic fibrosis (CF). Progress in CF clinical setting using the lipidic formulation GL67A has demonstrated the relevance of such a strategy while emphasizing the need for more potent gene transfer agents. In recent years, many novel non-viral gene delivery vehicles were proposed as potential alternatives to GL67 cationic lipid. However, they were usually evaluated using procedures difficult or even impossible to implement in clinical practice. In this study, a clinically-relevant administration protocol via aerosol in murine lungs was used to conduct a comparative study with GL67A. Diverse lipidic compounds were used to prepare a series of formulations inspired by the composition of GL67A. While some of these formulations were ineffective at transfecting murine lungs, others demonstrated modest-to-very-efficient activities and a series of structure-activity relationships were unveiled. Lipidic aminoglycoside derivative-based formulations were found to be at least as efficient as GL67A following aerosol delivery of a luciferase-encoding plasmid DNA. A single aerosol treatment with one such formulation was found to mediate long-term lung transgene expression, exceeding half the animal’s lifetime. This study clearly supports the potential of aminoglycoside-based cationic lipids as potent GL67-alternative scaffolds for further enhanced aerosol non-viral lung gene therapy for diseases such as CF.

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Section: Aerosol Deliverymentioning

confidence: 99%

Section: All Lipidic Formulations Were Not Stable When Mixed With Highly Concentrated Pdnamentioning

confidence: 99%

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Aerosol-Mediated Non-Viral Lung Gene Therapy: The Potential of Aminoglycoside-Based Cationic Liposomes

et al. 2021

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“…103 A study presented that nano-articulated silver carbene complexes enveloped have harmful affect for MDR bacteria involving P. aeruginosa, K. pneumoniae, MRSA, Acinetobacter baumannii (A. baumannii) and Burkholderia cepacia. 104 Ag-NM has been revealed to ensure antiviral action against HBV and HIV-1. 105 Zinc Oxide Nanomaterials (ZnO-NMs) Different tools are being utilized by ZnO-NMs to tackle microbes and making the resistant unlikely.…”

Section: Metallic Nanomaterials (Metallic-nms)mentioning

confidence: 99%

“…One of the examples is SCC's comprising L-Tyrosine polyphosphate NMs (LTP-NMs). 104 In vitro, antimicrobial action against C. albicans, B. cepacia, P. aeruginosa, MRSA, K. pneumoniae, and multidrug-resistant A. baumannii (MRAB) is shown by SCCs loaded into LTP-NMs and free SCCs. 18 Hindi et al studied that two inhaled doses administered about 72 hours of SCCs comprising LTP-NMs lower bacterial load in the lung, minimize bacteremia, and high survival by 25% in mouse models with P. aeruginosa pneumoniae.…”

Section: Nms Combating Intracellular Bacteriamentioning

confidence: 99%

<p>Recent Advances in Nanotechnology-Aided Materials in Combating Microbial Resistance and Functioning as Antibiotics Substitutes</p>

Munir

Ahmed

Usman

et al. 2020

IJN

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The ongoing escalation of drug-resistant bacteria creates the leading challenges for human health. Current predictions show that deaths due to bacterial illness will be more in comparison to cancer in 2050. Irrational use of antibiotics, prolonged regimen and using as a prophylactic treatment for various infections are leading cause of microbial resistance. It is an emerging approach to introduce evolving nanomaterials (NMs) as a base of antibacterial therapy to overcome the bacterial resistance pattern. NMs can implement several bactericidal ways and turn into a challenge for bacteria to survive and develop resistance against NMs. All the pathways depend on the surface chemistry, shape, core material and size of NMs. Because of these reasons, NMs based stuff shows a critical role in advancing the treatment efficiency by interacting with the cellular system of bacteria and functioned as an antibiotic substitute. We divided this review into two sections. The first part highlights the development of microbial resistance to antibiotics and their mechanisms. The second section details the NMs mechanisms to combat antibiotic resistance. In short, we try to summarize the advances in NMs role to deal with microbial resistance and giving solution as antibiotics substitute.

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Solvato‐Controlled Assembly and Structural Transformation of Emissive Poly‐NHC‐Based Organometallic Cages and Their Applications in Amino Acid Sensing and Fluorescence Imaging

Zhang

et al. 2023

Chemistry A European J

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Stimuli-induced structural transformation of supramolecular cages has drawn increasing attention because of their sensitive feature to external variations as model systems to simulate biological processes. However, combining structural transformation and useful functions has remained a difficult task. This study reports the solvato-controlled selfassembly of two unique topologies with different emission characteristics, a water-soluble Ag 8 L 4 cage (A) and an Ag 4 L 2 cage (B), produced from the same sulfonate-pendant tetraphenylethene (TPE) bridged tetrakis-(1,2,4-triazolium) ligand. Both cages show interesting solvent-responsive reversible structural transformation, and the change of fluorescence signals can efficiently track the process. Additionally, watersoluble cage A exhibits unique properties in thermochromism, thiol amino acid sensing, and subcellular imaging in aqueous media.

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Antibacterial and transfection activities of nebulized formulations incorporating long n-alkyl chain silver N-heterocyclic carbene complexes

Cited by 19 publications

References 48 publications

Aerosol-Mediated Non-Viral Lung Gene Therapy: The Potential of Aminoglycoside-Based Cationic Liposomes

Aerosol-Mediated Non-Viral Lung Gene Therapy: The Potential of Aminoglycoside-Based Cationic Liposomes

<p>Recent Advances in Nanotechnology-Aided Materials in Combating Microbial Resistance and Functioning as Antibiotics Substitutes</p>

Solvato‐Controlled Assembly and Structural Transformation of Emissive Poly‐NHC‐Based Organometallic Cages and Their Applications in Amino Acid Sensing and Fluorescence Imaging

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