Antibacterial Comparative Study Between Extracts of Mexican Propolis and of Three Plants Which Use Apis mellifera for its Production

Orozco, Amparo Londoño; Acevedo, José Guillermo Avila; Martínez, Ma. Margarita Canales; Hernandez-Delgado, Tzasna; Serrano, P. Rocio; Ortiz, Catalina; Díaz, Ángel Durán; Penieres‐Carrillo, Jose Guillermo; Tovar, Carlos Gerardo García; Sánchez, Tonatiuh Alejandro Crúz

doi:10.3923/javaa.2010.1250.1254

Cited by 22 publications

(7 citation statements)

References 12 publications

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“…They were then mixed, and this mixture was used as the propolis ointment for application to the dogs. By HPLC analysis, the main propolis constituents had previously been identified as flavones, cinnamic acid derivative, and caffeic acid derivative [14]. The ointment was applied once daily to the carefully washed and dried lesions, for three weeks for cases 1 and 2 and eight weeks for case 3.…”

Section: Treatmentmentioning

confidence: 99%

Use of Propolis for Topical Treatment of Dermatophytosis in Dog

Sánchez¹,

García²,

Zamora³

et al. 2014

OJVM

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Here we present three clinical cases of canine dermatophytosis resolved with topical propolis treatment that involved alopecia and well-demarcated erythematous lesions. These cases were positively identified by direct observation of samples from the affected zones with 10% KOH. Each sample was cultured, leading to the isolation of Microsporum gypseum in one case and Microsporum canis in the other two cases. The animals' subsequent treatment included bathing using a commercial soap with propolis every seven days for 3 to 8 weeks, as well as the use of a propoliscontaining ointment elaborated in our laboratory, which was applied to the lesions once a day for three weeks. From the second week of treatment, all cultures were negative. At the end of treatment, all cases displayed full recovery of the injuries and hair growth in these areas. In these clinical cases, treatment with propolis was effective, supporting the use of propolis as a promising natural alternative with no known collateral effects.

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Section: Treatmentmentioning

confidence: 99%

Use of Propolis for Topical Treatment of Dermatophytosis in Dog

Sánchez¹,

García²,

Zamora³

et al. 2014

OJVM

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“…Treatment in all cases lasted 4 weeks. Propolis' active substances were determined by HPLC analisis, the most relevant being flavones, cinnamic acid and caffeic acid [10]. Both propolis-based products were supplied by the Multidisciplinary Research Unit of the FES-Cuautitlán, UNAM.…”

Section: Treatmentmentioning

confidence: 99%

Use of Mexican Propolis for the Topical Treatment of Dermatomycosis in Horses

Rodríguez¹,

Monteagudo²,

Orozco³

et al. 2016

OJVM

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Five horses with lesions compatible with dermatomycosis were selected and skin scrapings were taken from all affected areas. Samples were then treated with 10% KOH and observed by direct microscopic examination; fungal cultures using Mycobiotic Agar and Borelli Agar were also preformed; Trichophyton mentagrophytes was identified in two cases, whereas Candida albicans was identified in the other three. Skin lesions in cases 1 and 2 were treated topically with ketoconazol ointment, while in the remaining cases a weekly bath with propolis-based shampoo and application of propolis-based ointment on the lesions 2 to 3 times a week were implemented. Treatment in all cases lasted 4 weeks. Pictures were taken before and after treatment and, making use of the Image Pro-Express ® software, therapy efficacy was determined by measurement of lesion area diameter and a significant reduction in lesion size was observed by the end of the 4 weeks of treatment with ketoconazole and propolis-based products. Thus proving the effectiveness of such propolis-based products, making them a natural therapeutic alternative for the treatment of cutaneous mycoses in horses, and carrying none of the toxic side effects of conventional pharmaceuticals.

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“…Al propóleo recolectado por las abejas domésticas (Apis mellifera) se le atribuyen efectos benéficos sobre la salud humana, por contar con propiedades biocidas contra bacterias (Londono et al, 2010;Bankova et al, 2014;Osés et al, 2016), hongos (Bankova et al, 2014;Sotero et al, 2015;Osés et al, 2016), protozoos (Nina et al, 2016a) y virus (González et al, 2015;Yildirim et al, 2016). Además, ha sido reportada su acción antiinflamatoria, antioxidante (Bilgin et al, 2016;Osés et al, 2016) y anticancerígena (Bankova et al, 2014).…”

Section: Introductionunclassified

“…Dada (Heon et al, 2008;Londono et al, 2010;Carrillo et al, 2011;Kim y Chung, 2011;Gavanji et al, 2012;Siripatrawan et al, 2013;Osés et al, 2016), donde los resultados han sido variables aun tratándose del mismo microorganismo.…”

Section: Introductionunclassified

Actividad antibacteriana de un extracto acuoso de propóleo del municipio de Irapuato, Guanajuato, México.

Bucio-Villalobos

Martínez-Jaime

2016

Agron. Mesoam.

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El objetivo del presente trabajo fue evaluar la actividad antibacteriana de un extracto acuoso de propóleo. En febrero de 2015 se colectó el propóleo en un apiario ubicado en Irapuato, Guanajuato, México. 300 g de propóleo fueron fraccionados y adicionados a 500 ml de etanol al 70%, en agitación continua por quince días. Por ltración al vacío, se separó la fase líquida, y el etanol contenido en ella fue evaporado en un rotoevaporador accionado a noventa revoluciones por minuto (rpm) y 80 oC. El residuo nal fue resuspendido en agua destilada, obteniéndose así el extracto acuoso. Un extracto de propóleo obtenido con etanol concentrado fue utilizado como control de referencia, además se incluyeron controles de etanol y agua. La actividad antibacteriana de los extractos fue evaluada mediante la técnica de difusión en agar, utilizando cepas de Escherichia coli (ATCC-10536), Salmonella typhimurium (ATCC-13311), Listeria monocytogenes (ATCC-19115) y Staphylococcus aureus (ATCC-11632), desarrolladas en tripticaseina-soya- agar, incubadas a 37 °C por veinticuatro horas (h) y se midió el halo de inhibición al término de dicho tiempo. Solo el extracto etanólico usado como control tuvo actividad antimicrobiana contra S. aureus y L. monocytogenes, más no se encontró respuesta para E. coli y S. typhimurium. El extracto acuoso no tuvo efecto sobre ninguna de las cuatro bacterias evaluadas, concluyéndose que los ingredientes activos del propóleo no son solubles en agua, por lo que se pierden en un proceso de extracción acuosa.

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Antibacterial Comparative Study Between Extracts of Mexican Propolis and of Three Plants Which Use Apis mellifera for its Production

Cited by 22 publications

References 12 publications

Use of Propolis for Topical Treatment of Dermatophytosis in Dog

Use of Propolis for Topical Treatment of Dermatophytosis in Dog

Use of Mexican Propolis for the Topical Treatment of Dermatomycosis in Horses

Actividad antibacteriana de un extracto acuoso de propóleo del municipio de Irapuato, Guanajuato, México.

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