Antibacterial Effect of Diode Laser in Pulpectomy of Primary Teeth

Abstract

Introduction: Laser irradiation has been suggested as an adjunct to traditional methods of canal preparation but few studies are available on the antibacterial effect of diode laser in pulpectomy of primary teeth. The purpose of the present study is to investigate the antibacterial effect of diode laser in pulpectomy of primary teeth, in addition to define the optimal and harmless diode lasing conditions in the root canal. Methods: A total of 125 single rooted primary teeth were selected. After traditional canal cleaning, they were divided in 2 groups. Sixty-five specimens after culturing of Enterococcus faecalis into the canals, were divided in 3 groups: (1) traditional canal cleaning with 0.5% NaOCl irrigation, (2) method of group 1+ 1.5 W diode laser (980 nm, pulse), (3) without treatment (5 specimens). Then the specimens were cultured and after colony counting under light microscope, were statistically analyzed by Kruskal-Wallis and Mann-Whitney tests. For 60 specimens, temperature rise of apical and cervical parts of the external root surface were measured using 2 thermocouple type K, when radiating a 1.5 W diode laser into the canal. Results: In the first experiment, the diode laser group showed tmost reduction in bacterial count. And in the second experiment, the mean temperature rise of external root surface was less than the threshold of periodontal ligament (PDL) damage. Conclusion: Diode laser with a power output of 1.5 W, is effective in reduction of E. faecalis bacterial count without damaging periodontal structures.

Aplicaciones del láser de alta po-tencia en odontología pediátrica

“…En pulpectomía, podemos certificar su habilidad para la desinfección de los conductos radiculares, habiendo presentado gran tasa de éxito en la disminución bacteriana de streptococcus fecalis al aplicarse un láser de diodo de 980nm 30 .…”

“…Diversos estudios han demostrado que el uso de láser de diodo, Er: YAG, y; Er, Cr: YSGG han presentado valores satisfactorios en la desinfección y eliminación de los patógenos endodónticos 30 .…”

Estudo clínico longitudinal do uso do laser diodo 980 nm no retratamento dos canais radiculares: efeito antimicrobiano e no reparo ósseo periapical

“…Diferentes lasers de alta potência têm sido testados com o intuito de melhorar o processo de desinfecção de canais radiculares (Bago et al, 2013;Ghorbanzadeh et al, 2016;Lu e Zhong, 2017;Bahrololoomi et al, 2017;Araujo et al, 2018;Lopes et al, 2018;Shaktawat et al, 2018), devido à alta capacidade de penetrar na estrutura dentinária e destruição bacteriana, além da sua capacidade de vaporizar tecido mole (Esteves-Oliveira et al, 2010;Ahangari et al, 2017;Alonaizan e AlFawaz, 2019).…”

“…Estudos in vitro demonstraram o efeito positivo da irradiação com o laser diodo na faixa de 810 -980 nm sobre a microbiota presente em infecções de canais radiculares (Gutknecht et al, 2004;Schoop et al, 2006;Romeo et al, 2015;Jyotsna et al, 2016;Sohrabi et al, 2016;Asnaashari et al, 2016a;Bahrololoomi et al, 2017;Shaktawat et al, 2018).…”

“…O laser diodo 980 nm transmite energia por meio de fibras flexíveis que são compatíveis com as dimensões e formas curvas dos canais radiculares (Alfredo et al, 2009;Faria et al, 2011;Faria et al, 2013). Esse comprimento de onda é capaz de penetrar nos túbulos dentinários (Romeo et al, 2015;Lopes et al, 2016;Sohrabi et al, 2016;Araujo et al, 2018;Bahrololoomi et al, 2017;Shaktawat et al, 2018), atuando com efeito bactericida até 500 μm (Gutknecht et al, 2004;Marchesan et al, 2008;Asnaashari et al, 2016a), profundidade que a solução irrigante não pode alcançar.…”