Antibacterial Effectiveness of 2% Chitosan and 2% Chlorhexidine Against Enterococcus Faecalis in Biofilm (Laboratory Experiment)

Simanjuntak, Priscilla Arlyta; Djauharie, Nilakesuma; Nursasongko, Bambang

doi:10.22159/ijap.2019.v11s1.163

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…It can be used as a temporary scaffolding support for tissue growth and regeneration. 6 7 However, chitosan has two major limitations: it is not soluble in water and it has a low pH. The chitosan formulation used in this study was chitosan derivative (water-soluble chitosan) with lower molecular weight and has been developed to dissolve in water.…”

Section: Introductionmentioning

confidence: 99%

Characterization, Physical Properties, and Biocompatibility of Novel Tricalcium Silicate–Chitosan Endodontic Sealer

et al. 2022

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Objective The purpose of this study was to compare the characteristics, physical properties, and biocompatibility of the novel tricalcium silicate–chitosan (TCS-C) sealer with AH Plus and Sure-Seal Root. Materials and Methods The TCS-C powder was prepared by mixing tricalcium silicate with 2% water-soluble chitosan at a 5:1 ratio, followed by sufficient addition of 10 g/mL ratio of double-distilled water to form a homogeneous cement. Material characterizations (the Fourier Transform InfraRed [FTIR] and X-ray diffraction [XRD]), physical property investigations (flow and film thickness), and cytotoxicity tests in 3T3 mouse embryo fibroblast cell (MTT assay method) were performed on sealers, and the results were compared with those of the commercial products. Statistical Analysis Statistical analysis was performed on flow and film thickness. The normality of the data was tested using the Shapiro–Wilk test. Statistical analysis was performed with one-way analysis of variance (ANOVA). The level of significance was set at p < 0.05. Results The TCS-C showed a mean flow of 31.98 ± 0.68 mm, compared with Sure Seal Root at 26.38 ± 0.69 mm and AH Plus at 26.50 ± 0.12 mm. The TCS-C showed a mean film thickness of 60 ± 10.0 mm compared with Sure-Seal Root at 50 ± 10.0 mm and AH Plus at 40 ± 15.8 mm. The TCS-C exhibited low to no cytotoxicity in fibroblast cell at all concentrations and exposure times. Conclusion Adding water-soluble chitosan may improve the physical and biologic properties of tricalcium silicate cement. The novel TCS-C sealer did not fully meet the physical properties of an endodontic sealer, but it was not cytotoxic to fibroblast cells.

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Section: Introductionmentioning

confidence: 99%

Characterization, Physical Properties, and Biocompatibility of Novel Tricalcium Silicate–Chitosan Endodontic Sealer

et al. 2022

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“…[ 9 10 11 12 ] Its antibacterial activity against total bacteria and E. faecialis has been found to be comparable to chlorhexidine mouthwash. [ 10 13 ] Therefore, this study sought to evaluate the cytotoxicity effect of miniscrews exposed to different types of mouthwash using human gingival fibroblast (HGFs).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity effect of orthodontic miniscrew-implant in different types of mouthwash

Anggani

Utami

Purbiati

2022

Journal of Orthodontic Science

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CONTEXT: Orthodontic miniscrew implants (OMIs) are widely used as anchorage alternatives, but recent studies revealed the corrosion behavior of OMIs when they come in contact with mouthwashes. The corrosion materials that are released can cause toxicity, allergy, and mutagenicity. AIMS: This study aims to analyze the cytotoxicity effects of OMIs exposed to different types of mouthwash using human gingival fibroblast (HGFs). SETTINGS AND DESIGN: Experimental laboratory research. METHODS AND MATERIAL: Twenty-eight samples of Ti alloy OMIs immersed separately in four groups of different types of mouthwash (chlorhexidine gluconate 0.2% mouthwash (CHX), fluoridated (sodium fluoride 0.2%) mouthwash, chitosan mouthwash 1.5%, and aquadest) for 28 d. Elution of each group and the mouthwash itself were added to the cell culture and incubated for 24 h. Changes in cell viability were performed by MTT Assay. STATISTICAL ANALYSIS USED: Data were tested for normality with Shapiro–Wilk, homogeneity with Levene test, and analyzed using an independent T-test ( P < 0.05). RESULTS: The differences between the cytotoxicity of the elution of MIO and the mouthwash solution itself in the group of CHX and Fluoride were statistically significant ( P < 0.05). No significant differences were found in the group of chitosan and aquadest ( P > 0.05). CONCLUSIONS: The 1.5% chitosan mouthwash can be offered to patients with Ti alloy-based OMIs rather than the 0.2% chlorhexidine gluconate and 0.2% sodium fluoride mouthwashes.

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“…Chitosan is a non-toxic natural polysaccharide generated from the deacetylation of chitin, which is attained from the shells of crustaceans and shrimp [12]. Previous studies have elucidated that chitosan has antibacterial properties against E faecalis and C albicans [13,14].…”

Section: Introductionmentioning

confidence: 99%

Setting Time, Flowability, and Solubility of Epoxy Resin-Based Sealer Mixed With Chitosan Nanoparticles

et al. 2021

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Objective: The purpose of this study was to investigate the effect of the incorporation of chitosan nanoparticles into epoxy resin-based sealer on its setting time, flowability and solubility. Methods: This study was divided into three evaluations: setting time, flowability, and solubility of sealers. Each study used 20 samples, which were divided into two groups. Group 1, epoxy resin-based sealers, and group 2, epoxy resin-based sealers mixed with chitosan nanoparticles. The Gilmore needle was used to observe the setting time, a simple press method based on ISO 6876 was employed to evaluate the flowability of the sealer, and the solubility test, which was according to ISO 6876 standard, was used to determine the solubility of sealers. The surface structure of the sealers before and after the solubility test was observed under Scanning Electron Microscopy (SEM). The data obtained from each study were analyzed by an unpaired t-test with a degree of significance of 95%. Results: The results showed that the setting time of epoxy resin and epoxy resin incorporated with chitosan were 567±30.20 and 572.5±27.91 min, flowability was 25.06±0.89 and 23.18±1.06 mm, and solubility was 0.0051%±0.0016 and 0.0045%±0.0018, respectively. No significant difference occurred between epoxy resin-based sealer and epoxy resin-based sealer mixed with chitosan nanoparticles in setting time, flowability, and solubility of sealers (*P>0.05). Conclusion: The incorporation of chitosan nanoparticles produced a similar effect in setting time, flowability, and solubility as the epoxy resin-based sealer. Thus, epoxy resin-based sealer mixed with chitosan nanoparticles had acceptable properties in setting time, flowability, and solubility based on ISO 6876 standards.

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Antibacterial Effectiveness of 2% Chitosan and 2% Chlorhexidine Against Enterococcus Faecalis in Biofilm (Laboratory Experiment)

Cited by 6 publications

References 29 publications

Characterization, Physical Properties, and Biocompatibility of Novel Tricalcium Silicate–Chitosan Endodontic Sealer

Characterization, Physical Properties, and Biocompatibility of Novel Tricalcium Silicate–Chitosan Endodontic Sealer

Cytotoxicity effect of orthodontic miniscrew-implant in different types of mouthwash

Setting Time, Flowability, and Solubility of Epoxy Resin-Based Sealer Mixed With Chitosan Nanoparticles

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