Antibacterial effects of silver nanoparticles on gram-negative bacteria: Influence on the growth and biofilms formation, mechanisms of action

Radzig, Marina A.; Надточенко, В. А.; Кокшарова, О. А.; Kiwi, J.; Липасова, В. А.; Khmel, I. A.

doi:10.1016/j.colsurfb.2012.07.039

Cited by 425 publications

(265 citation statements)

References 34 publications

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“…Antibiofilm action of AgNPs of 8.3 nm in diameter stabilized by hydrolyzed casein peptides on Gram-negative bacteria (E. coli, P. aeruginosa and Serratia proteamaculans) was investigated by Radzig et al [86]. A strong inhibition of biofilms formation was observed.…”

Section: Agnps Antibiofilm Activitymentioning

confidence: 99%

“…Porins are involved in the transport of Ag-ions and E. coli bacteria expressing mutated porin proteins are less susceptible to silver ions action [87]. Radzig et al [86] confirmed that E. coli mutant strains deficient in OmpF or OmpC porins were 4-8 times more resistant to AgNP when compared to the wild type strain, suggesting that porins have a key role in allowing AgNPs to exert their antibacterial effect. The anti-biofilm activity of silver nanoparticles was also demonstrated in other studies mainly focused on bacteria showing resistance to conventional antibiotics [88,89].…”

Section: Agnps Antibiofilm Activitymentioning

confidence: 99%

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Silver Nanoparticles as Potential Antibacterial Agents

et al. 2015

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Multi-drug resistance is a growing problem in the treatment of infectious diseases and the widespread use of broad-spectrum antibiotics has produced antibiotic resistance for many human bacterial pathogens. Advances in nanotechnology have opened new horizons in nanomedicine, allowing the synthesis of nanoparticles that can be assembled into complex architectures. Novel studies and technologies are devoted to understanding the mechanisms of disease for the design of new drugs, but unfortunately infectious diseases continue to be a major health burden worldwide. Since ancient times, silver was known for its anti-bacterial effects and for centuries it has been used for prevention and control of disparate infections. Currently nanotechnology and nanomaterials are fully integrated in common applications and objects that we use every day. In addition, the silver nanoparticles are attracting much interest because of their potent antibacterial activity. Many studies have also shown an important activity of silver nanoparticles against bacterial biofilms. This review aims to OPEN ACCESSMolecules 2015, 20 8857 summarize the emerging efforts to address current challenges and solutions in the treatment of infectious diseases, particularly the use of nanosilver antimicrobials.

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Section: Agnps Antibiofilm Activitymentioning

confidence: 99%

Section: Agnps Antibiofilm Activitymentioning

confidence: 99%

Silver Nanoparticles as Potential Antibacterial Agents

et al. 2015

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“…The samples were analyzed on a flow cytometer (BD FACS Verse™ BD Biosciences, San Jose, CA, USA) to discriminate between live and dead cells. 29,30 The above stained samples were also visualized using fluorescent microscopy at 1,000× magnification.…”

Section: Viability Analysis Of Pbsnps-treated P Aeruginosa Pao1mentioning

confidence: 99%

Facile biofunctionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal, and biofilm control

Lambadi¹,

Sharma²,

Kumar³

et al. 2015

IJN

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Infectious diseases cause a huge burden on healthcare systems worldwide. Pathogenic bacteria establish infection by developing antibiotic resistance and modulating the host’s immune system, whereas opportunistic pathogens like Pseudomonas aeruginosa adapt to adverse conditions owing to their ability to form biofilms. In the present study, silver nanoparticles were biofunctionalized with polymyxin B, an antibacterial peptide using a facile method. The biofunctionalized nanoparticles (polymyxin B-capped silver nanoparticles, PBSNPs) were assessed for antibacterial activity against multiple drug-resistant clinical strain Vibrio fluvialis and nosocomial pathogen P. aeruginosa . The results of antibacterial assay revealed that PBSNPs had an approximately 3-fold higher effect than the citrate-capped nanoparticles (CSNPs). Morphological damage to the cell membrane was followed by scanning electron microscopy, testifying PBSNPs to be more potent in controlling the bacterial growth as compared with CSNPs. The bactericidal effect of PBSNPs was further confirmed by Live/Dead staining assays. Apart from the antibacterial activity, the biofunctionalized nanoparticles were found to resist biofilm formation. Electroplating of PBSNPs onto stainless steel surgical blades retained the antibacterial activity against P. aeruginosa . Further, the affinity of polymyxin for endotoxin was exploited for its removal using PBSNPs. It was found that the prepared nanoparticles removed 97% of the endotoxin from the solution. Such multifarious uses of metal nanoparticles are an attractive means of enhancing the potency of antimicrobial agents to control infections.

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“…The disrupted cellular membranes could facilitate the entry of nanoparticles into the host cell and several reports have demonstrated that Gram-negative bacteria show an increased susceptibility to AgNPs (Sondi & Salopek-Sondi, 2004;Radzig et al, 2013), which makes these vehicles suitable candidates as an antimicrobial agent against NHP-B. The application of nanoparticles to prevent bacterial colonization in human cells has been explored, but no progress has been reported against NHP-B in aquaculture species.…”

Section: _____________________mentioning

confidence: 99%

Antibacterial effect of biosynthesized silver nanoparticles in Pacific white shrimp Litopenaeus vannamei (Boone) infected with necrotizing hepatopancreatitis bacterium (NHP B)

Acedo-Valdez

Chon

Larios-Rodríguez

et al. 2017

lajar

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ABSTRACT. The shrimp necrotizing hepatopancreatitis is caused by a pleomorphic Gram-negative Rickettsialike bacteria known as NHP-B. It is well known that silver is an effective bactericidal, but experiments with aquatic organisms are scarce, even more at nanoparticle level. The objective of this study was to determine the antibacterial effect of silver nanoparticles (AgNP) against NHP-B in the Pacific white shrimp Litopenaeus vannamei. For this, AgNO3 was used as source of silver, and extracts of dried leaves of green tea Camellia sinensis and neem tree Azadirachta indica as reducing agents. Different lots of infected shrimp received 0,5 and 35 μg of AgNP by forced feeding. The differences between the number of bacterial nodules in shrimp hepatopancreas and shrimp mortality relative to the amount of AgNP demonstrated to be effective against this pathogen.

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Antibacterial effects of silver nanoparticles on gram-negative bacteria: Influence on the growth and biofilms formation, mechanisms of action

Cited by 425 publications

References 34 publications

Silver Nanoparticles as Potential Antibacterial Agents

Silver Nanoparticles as Potential Antibacterial Agents

Facile biofunctionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal, and biofilm control

Antibacterial effect of biosynthesized silver nanoparticles in Pacific white shrimp Litopenaeus vannamei (Boone) infected with necrotizing hepatopancreatitis bacterium (NHP B)

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