Antibacterial Effects of Two Different Types of Laser and Aqueous Ozone Against<i>Enterococcus faecalis</i>in Root Canals

Zan, Recai; Hubbezoğlu, İhsan; Sümer, Zeynep; Tunç, Tutku; Tanalp, Jale

doi:10.1089/pho.2012.3417

Cited by 17 publications

(18 citation statements)

References 25 publications

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“…In particular; 5.25% NaOCl solution has shown the strongest bactericidal efficacy in eliminating all microorganisms in root canals and deeper dentinal tubules. Therefore, 5.25% NaOCl is recommended as an effective solution in the treatment of infected root canals due to its well-known antimicrobial effects (18-22). In the present study, we used 5.25% NaOCl in root canals as a positive control group.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, we used 5.25% NaOCl in root canals as a positive control group. No wonder, when complete bacterial elimination was achieved, as indicated by aforementioned researches (18-22). …”

Section: Discussionmentioning

confidence: 99%

“…In general, one of the resistant microorganisms that has reduced endodontic treatment success and been isolated from root canals is E. faecalis (20-22). Therefore, we used this bacterium to obtain more realistic clinical results.…”

Section: Discussionmentioning

confidence: 99%

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Antibacterial Efficacy of Aqueous Ozone in Root Canals Infected by Enterococcus faecalis

Hubbezoğlu

Zan

Tunç

et al. 2014

Jundishapur J Microbiol

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Background:In endodontics, the elimination of resistant bacteria such as Enterococcus faecalis plays an important role for treatment success in root canals. Therefore, new alternative irrigants (instead of sodium hypochlorite) have been researched to achieve ideal endodontic treatment.Objectives:The aim of the present study was to evaluate and to compare the antibacterial effect of aqueous ozone with different concentrations and techniques of application (manual and ultrasonic) against E. faecalis in human root canals.Patients and Methods:Eighty single-root mandibular premolar teeth were selected, prepared and sterilized. E. faecalis was incubated in the root canals and kept at 37°C for 24 h. The teeth were divided into four main groups each has 20 members: NaOCl (positive control) group; 8 ppm aqueous ozone group; 12 ppm aqueous ozone group; and 16 ppm aqueous ozone group. While half of the specimens were disinfected with aqueous ozone by manual technique, the other half was disinfected with the aqueous ozone by ultrasonic technique. Conventional irrigation technique was simultaneously applied with ultrasonic vibration that was produced by VDW.ULTRA device. The disinfection procedures were performed for 180 s to ensure standardization of all the working groups. Paper points (placed in the root canals before and after the disinfection procedures) were transferred to Eppendorf tubes containing 0.5 mL of brain heart infusion broth. Then, 50 μL of the suspension was inoculated onto broth agar media. Microbial colonies were counted, and the data were evaluated statistically using 2-way analysis of variance (ANOVA) and Tukey tests.Results:Although the antibacterial effect of 16 ppm aqueous ozone using a manual technique had an insufficient effect, its ultrasonic application technique resulted in complete disinfection in the root canals.Conclusions:The bactericidal activity of high concentration of aqueous ozone combined with ultrasonic application technique showed efficacy similar to that of 5.25% NaOCl in root canals.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Antibacterial Efficacy of Aqueous Ozone in Root Canals Infected by Enterococcus faecalis

Hubbezoğlu

Zan

Tunç

et al. 2014

Jundishapur J Microbiol

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“…In the present study, 5.25% NaOCl was applied as a negative control group to compare the antibacterial efficacy of experimental groups against P. aeruginosa in root canals. Consequently, the application of 5.25% NaOCl achieved exact sterilization in root canals like in the above mentioned studies (Baumgartner and Cuenin 1992;Huth et al, 2009;Kustarcı et al, 2009, Zan et al, 2013.…”

Section: Discussionmentioning

confidence: 87%

“…The most commonly prefered irrigation solution is 5.25% sodium hypochlorite (NaOCl). Recent researches demonstrated its strong antibacterial efficacy in infected root canals (Baumgartner and Cuenin, 1992;Huth et al, 2009;Kustarcı et al, 2009;Zan et al, 2013). Furthermore, the bactericidal ability of NaOCl occurred because of the formation of hypochlorous acid (HOCl), when it comes in contact with organic debris.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial effect of gaseous and aqueous ozone in root canals contaminated with Pseudomonas aeruginosa

Hubbezoğlu¹,

Zan²,

Sümer³

et al. 2013

Afr. J. Microbiol. Res.

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This present study attempted to identify the antibacterial effects of aqueous and gaseous ozone in human root canals that are contaminated with Pseudomonas aeruginosa. The differences and antibacterial effects of gaseous and aqueous ozone (manual and ultrasonic) have not been compared to each other yet in any studies. Eighty single-root premolar teeth were prepared and then disinfected and sterilized. P. aeruginosa were incubated in root canals and kept at 37°C for 24 h. The root canals were contaminated with P. aeruginosa divided into 1 positive control, sodium hypochlorite (NaOCl) and three experimental groups: aqueous ozone with manual technique, aqueous ozone with ultrasonic technique and gaseous ozone (n=10). Disinfection procedures were performed for 5 min in order to ensure standardization among all working groups. Remaining microorganism colonies were counted on blood agar plates. Then, data were evaluated and statistically analyzed by one-way ANOVA and Tukey's test. As a result, although there were no statistically significant differences between the three groups (NaOCl, aqueous ozone with manual, and ultrasonic technique) (P>0.05), there were statistically significant differences between the gaseous ozone and all other groups (P<0.05). Both the manual and ultrasonic techniques of aqueous ozone achieved complete elimination of P. aeruginosa in root canals.

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Ozone Application in Endodontics

Mohammadi

Azarpazhooh

2015

Endodontic Irrigation

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Antibacterial Effects of Two Different Types of Laser and Aqueous Ozone AgainstEnterococcus faecalisin Root Canals

Abstract: The results showed that when aqueous ozone was applied with the aim of disinfecting the root canals, it exhibited a higher antibacterial effect than the KTP and Er:YAG lasers. However, the antibacterial effect of the aqueous ozone was insufficient when compared with NaOCl.

Cited by 17 publications

References 25 publications

Antibacterial Efficacy of Aqueous Ozone in Root Canals Infected by Enterococcus faecalis

Antibacterial Efficacy of Aqueous Ozone in Root Canals Infected by Enterococcus faecalis

Antibacterial effect of gaseous and aqueous ozone in root canals contaminated with Pseudomonas aeruginosa

Ozone Application in Endodontics

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