Antibacterial ent-kaurene from Brazilian propolis of native stingless bees

Velikova, Milena; Bankova, Vassya; Tsvetkova, Iva; Kujumgiev, A; Marcucci, María Cristina

doi:10.1016/s0367-326x(00)00213-6

Cited by 108 publications

(69 citation statements)

References 12 publications

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“…Initial GC-MS studies of propolis focused on the more volatile constituents of propolis, or necessarily undertook derivatization of the extracts [13][14][15] as a sample preparation procedure. Furthermore, a single sample could take an hour to be analyzed.…”

Section: Resultsmentioning

confidence: 99%

“…GC/MS was used to analyze content of propolis of stingless bees from Brazil [13][14][15] and Mexico [16] showing the composition of native bee propolis to be different from that of A. mellifera bees from the same regions. In more recent studies [17,18], negative ion mode eletrospray ionization-mass spectrometry [ESI(−)-MS] fingerprinting was used to compare the composition of ethanolic extracts of samples of native Brazilian stingless bee propolis with extracts of possible plant sources.…”

Section: Introductionmentioning

confidence: 99%

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Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

Sawaya

Abdelnur²,

Eberlin³

et al. 2010

Talanta

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a b s t r a c tChemical profiles of a representative set of 49 propolis ethanolic extracts collected worldwide (North and South America, Europe, Asia and Oceania) were obtained via easy ambient sonic-spray ionization mass spectrometry (EASI-MS). This simple and easily implemented fingerprinting technique analyses directly (without any pre-separation or sample manipulation) a tiny droplet of the ethanolic extract placed on a inert surface under ambient conditions. Data acquisition took about a minute per sample with no substantial sample carry-over. Extraction of propolis with ethanol by using an ultrasonic bath method gave EASI-MS data similar to the traditional maceration method, reducing total analysis time for the crude propolis resin from a week to just ca 1 h. Principal component analysis of the EASI-MS data is shown to group samples according to the plant sources of their resins, which characterizes their geographical origin.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fingerprinting of propolis by easy ambient sonic-spray ionization mass spectrometry

Sawaya

Abdelnur²,

Eberlin³

et al. 2010

Talanta

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“…11,12 Some works, among many found in the literature on the studies with propolis, mention the importance in the discovery of new molecules (new metabolites) and in the identification of the biological properties of substances obtained from propolis extracts. Velikova, 13 analyzing the samples of Brazilian propolis, collected in the municipal district of Prudentópolis in the State of Paraná, described the isolation of new diterpenes of the class of kaurenes. Kusumoto,14 described the isolation of new chemical constituents, terpenes and phenolic derived, from the essential oil obtained of propolis samples collected in the south of Minas Gerais, Brazil.…”

Section: Introductionmentioning

confidence: 99%

Chiral Analysis of Monoterpenes in Volatile Oils From Propolis

Simionatto

Facco

Morel

et al. 2012

J. Chil. Chem. Soc.

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The essential oils obtained by hydrodistillation from samples of propolis manufactured in three regions of Rio Grande do Sul State at Brazil, were analyzed through CG, CG-MS and chiral phase gas chromatography (CPGC). These analyses display the presence of samples with elevated essential oil purport, when compared with plants. The yields obtained were until 3.8%. The samples exhibited similar composition, with predominance of the monoterpenes a-pinene (57-63%), b-pinene (12.5-30.8%) and limonene (1.5-11.2%). In chiral analysis of these constituents were observed modifications in the enantiomeric excess of isomers of a-pinene and limonene in relation with source location of the sample, already in the chiral analysis of b-pinene were detected only elevated excess of the enantiomer with the (-) configuration. The antimicrobial activity of the crude essential oil was assayed against five bacteria. The best result was obtained against Staphylococcus aureus.

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“…Propolis in general contains a variety of chemical compounds such as polyphenols (flavonoid aglycones, phenolic acids, and their esters, phenolic aldehydes, alcohols, and ketones), terpenoids, steroids, amino acids, and inorganic compounds (Dimov et al, 1991;Volpert and Elstner, 1993;Moreno et al, 2000). Many biological properties, including antibacterial, (Mochida et al, 1985;Velikova et al, 2000), antifungal (Dimov et al, 1991;Murad et al, 2002), antiviral (Amoros et al, 1992;Amoros et al, 1994), anti-inflammatory (Strehl et al, 1994;Miyataka et al, 1997), antioxidant (Sun et al, 2000;Isla et al, 2001), hepatoprotective (Gonzales et al, 1995) and immunostimulating (Dimov et al, 1991) activities have been ascribed to propolis. Antibacterial activity of propolis is reported to be due to flavonoids, aromatic acids, and its esters.…”

Section: Introductionmentioning

confidence: 99%