Antibacterial interactions between two monofloral honeys and several topical antiseptics, including essential oils

Gray, Brayden H; Green, Kathryn J.; Haines, Robbie R.; Hammer, Katherine A.

doi:10.1186/s12906-022-03695-x

Cited by 1 publication

(1 citation statement)

References 39 publications

(52 reference statements)

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“…Following a previous study, the in vitro synergistic activities of antibiotic COL in conjunction with BT against COL-R GNB were evaluated through checkerboard assays. 51 Briefly, COL and BT were 2-fold serially diluted with CAMHB (Thermo Fisher Scientific) along the x-axis and y-axis, respectively. A drug mixture (100 μL) was formed in the 96-well plate.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Bithionol Restores Sensitivity of Multidrug-Resistant Gram-Negative Bacteria to Colistin with Antimicrobial and Anti-biofilm Effects

et al. 2023

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Being among the few last-resort antibiotics, colistin (COL) has been used to treat severe infectious diseases, such as those caused by multidrugresistant Gram-negative bacteria (MDR GNB). However, the appearance of colistin-resistant (COL-R) GNB has been frequently reported. Therefore, novel antimicrobial strategies need to be urgently sought to address this resistance challenge. In the present study, antimicrobial drug screening conducted revealed that bithionol (BT), approved by the Food and Drug Administration and used as an anthelminthic drug for paragonimiasis, exhibited a synergistic antibacterial effect with COL. Clinically isolated COL-R GNB were used as candidates to evaluate the synergistic antibacterial activity. The results revealed that BT could significantly reverse the sensitivity of COL-R GNB to COL. Furthermore, the combined application of BT and COL can reduce bacterial biofilm formation and have a scavenging effect on the mature biofilm in vitro. The damage caused to the bacterial cell membrane integrity by the BT/COL combination was observed under a fluorescence microscope. The fluorescence intensity of reactive oxygen species also increased in the experimental group. The BT/COL combination also exhibited a synergistic antibacterial effect in vivo. Importantly, BT was confirmed to be safe at the highest concentrations that exerted synergistic effects on all tested strains. In conclusion, our findings demonstrated that BT exerted synergistic antimicrobial and anti-biofilm effects when combined with COL against MDR organisms, especially COL-R GNB, in vitro and in vivo. The findings thus provide a reference for the clinical response to the serious challenge of MDR GNB and the exploitation of the potential antibacterial activities of existing clinical non-antibacterial drugs.

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Section: ■ Materials and Methodsmentioning

confidence: 99%