Antibacterial mechanism of oregano essential oil

Cui, Haiying; Zhang, Chenghui; Li, Changzhu; Lin, Lin

doi:10.1016/j.indcrop.2019.111498

Cited by 247 publications

(126 citation statements)

References 27 publications

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“…It was confirmed that GEO treatment caused the leakage of proteins in the bacterial cells by destroying the bacterial cell membrane, leading to a decrease in the protein content of the bacterial cells. In this way, it interferes with the synthesis of certain proteins and enzymes, resulting in the reduction of protein expression in bacterial cells, thus exerting its antibacterial activity [ 4 ]. The SDS-PAGE was used only to analyze the intracellular proteins (molecular weight) of two bacteria ( E. coli and S. aureus ) after treatment with different concentrations of GEO.…”

Section: Discussionmentioning

confidence: 99%

“…Another study showed that the treatment of EO can inhibit the activity of β-galactosidase, ATPase and ALP, which can be an important factor for resulting bacterial death [ 27 ]. It was reported that oregano EO can inhibit S. aureus respiratory metabolism by affecting the metabolites and key enzymes of TCA cycle [ 4 ]. Based on the results of transcriptional profiling experiments, we can preliminarily conclude that GEO could inhibit the microbial growth in several ways.…”

Section: Discussionmentioning

confidence: 99%

“…Among the various intrinsic determinant factors for food deterioration, the presence of certain pathogenic microorganisms (bacteria, fungi, and virus) can cause spoilage and decay which impede to socio-economic development. Under certain conditions, E. coli can cause gastrointestinal tract infections, urinary tract infections and other local tissue and organ infections [ 3 ]; Meanwhile, S. aureus as an opportunistic pathogen that can produce enterotoxins that lead to food poisoning [ 4 ]. Therefore, the quest for potential antibacterial agents with precise mechanisms of action has become an essential drive among food scientists.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus

et al. 2020

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Though essential oils exhibit antibacterial activity against food pathogens, their underlying mechanism is understudied. We extracted ginger essential oil (GEO) using supercritical CO2 and steam distillation. A chemical composition comparison by GC-MS showed that the main components of the extracted GEOs were zingiberene and α-curcumene. Their antibacterial activity and associated mechanism against Staphylococcus aureus and Escherichia coli were investigated. The diameter of inhibition zone (DIZ) of GEO against S. aureus was 17.1 mm, with a minimum inhibition concentration (MIC) of 1.0 mg/mL, and minimum bactericide concentration (MBC) of 2.0 mg/mL. For E. coli, the DIZ was 12.3 mm with MIC and MBC values of 2.0 mg/mL and 4.0 mg/mL, respectively. The SDS-PAGE analysis revealed that some of the electrophoretic bacterial cell proteins bands disappeared with the increase in GEO concentration. Consequently, the nucleic acids content of bacterial suspension was raised significantly and the metabolic activity of bacteria was markedly decreased. GEO could thus inhibit the expression of some genes linked to bacterial energy metabolism, tricarboxylic acid cycle, cell membrane-related proteins, and DNA metabolism. Our findings speculate the bactericidal effects of GEO primarily through disruption of the bacterial cell membrane indicating its suitability in food perseveration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus

et al. 2020

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“…Recent studies have reported on electrospun fibres containing oregano essential oils, extracted from Origanum vulgare [45][46][47] and Origanum minutiflorum [48], which are plants belonging to the Lamiaceae family and predominantly distributed in the Mediterranean, Euro-Siberian and Iran-Siberian areas. The major constituents of oregano EO are carvacrol and thymol, which have inhibitory effect on diverse microorganisms, including Methicillin-resistant S. aureus (MRSA), E. coli, Bacillus subtilis (B. subtilis), and Saccharomyces cerevisiae [79][80][81]. As other EOs, oregano EO acts on the bacteria cell membrane by disrupting its functions, inducing loss of cytosolic material and leakage of potassium ions, with eventual cell necrosis.…”

Section: Oregano Essential Oilmentioning

confidence: 99%

Electrospinning of Essential Oils

Mele

2020

Polymers

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The extensive and sometimes unregulated use of synthetic chemicals, such as drugs, preservatives, and pesticides, is posing big threats to global health, the environment, and food security. This has stimulated the research of new strategies to deal with bacterial infections in animals and humans and to eradicate pests. Plant extracts, particularly essential oils, have recently emerged as valid alternatives to synthetic drugs, due to their properties which include antibacterial, antifungal, anti-inflammatory, antioxidant, and insecticidal activity. This review discusses the current research on the use of electrospinning to encapsulate essential oils into polymeric nanofibres and achieve controlled release of these bioactive compounds, while protecting them from degradation. The works here analysed demonstrate that the electrospinning process is an effective strategy to preserve the properties of essential oils and create bioactive membranes for biomedical, pharmaceutical, and food packaging applications.

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“…Plant essential oils are a class of natural antimicrobial compounds with good antimicrobial activities against bacteria, viruses, and fungi ( Wattanasatcha et al., 2012 ; Marchese et al., 2016 ; Zhang et al., 2016 ; Cui et al., 2019 ). Secondary metabolites with a phenolic structure are the main antimicrobial compounds in these plant essential oils ( Wattanasatcha et al., 2012 ; Marchese et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis

Liu¹,

Panpan²,

Gong³

et al. 2020

Poultry Science

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The inhibitory and bactericidal activities of thyme oil against the foodborne multiple antibiotics-resistant Enterococcus faecalis biofilm were evaluated in this study. Gas chromatography-mass spectrometry revealed that more than 70% of the composition of thyme oil is thymol. Crystal violet staining assay showed that 128 and 256 μg/mL thyme oil significantly inhibited the biofilm formation of E. faecalis . The cell adherence of E. faecalis , as shown by its swimming and swarming motilities, was reduced by thyme oil. The exopolysaccharide ( EPS ) quantification assay showed that thyme oil inhibited the EPS synthesis in E. faecalis biofilms. The 3D-view observations through confocal laser scanning and scanning electron microscopy suggested that cell adherence and biofilm thickness were decreased in thyme oil–treated biofilms. Quantitative real-time analyses showed that the transcription of ebp and epa gene clusters, which were related to cell mobility and EPS production, was inhibited by thyme oil. Thus, thyme oil effectively inhibited the biofilm formation of E. faecalis by affecting cell adherence and EPS synthesis. Furthermore, 2,048 and 4,096 μg/mL thyme oil can effectively inactivate E. faecalis population in the mature E. faecalis biofilms by 5.75 and 7.20 log CFU/mL, respectively, after 30 min of treatment. Thus, thyme oil at different concentrations can be used as an effective antibiofilm or germicidal agent to control E. faecalis biofilms.

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Antibacterial mechanism of oregano essential oil

Cited by 247 publications

References 27 publications

Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus

Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus

Electrospinning of Essential Oils

Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis

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